iScience
Volume 7, 28 September 2018, Pages 30-39
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Article
Single-Cell Profiling Identifies Key Pathways Expressed by iPSCs Cultured in Different Commercial Media

https://doi.org/10.1016/j.isci.2018.08.016Get rights and content
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Highlights

  • HiPSCs maintained in StemFlex and E8 were compared by single-cell transcriptomics

  • Data revealed similar expression of core pluripotency genes

  • Data revealed a shared three subpopulation structure different in pluripotency states

Summary

We assessed the pluripotency of human induced pluripotent stem cells (iPSCs) maintained on an automated platform using StemFlex and TeSR-E8 media. Analysis of transcriptome of single cells revealed similar expression of core pluripotency genes, as well as genes associated with naive and primed states of pluripotency. Analysis of individual cells from four samples consisting of two different iPSC lines each grown in the two culture media revealed a shared subpopulation structure with three main subpopulations different in pluripotency states. By implementing a machine learning approach, we estimated that most cells within each subpopulation are very similar between all four samples. The single-cell RNA sequencing analysis of iPSC lines grown in both media reports the molecular signature in StemFlex medium and how it compares to that observed in the TeSR-E8 medium.

Subject Areas

Stem Cells Research
Transcriptomics
Automation

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11

These authors contributed equally

12

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