Basic ResearchIn Vitro Assessment of Mineral Trioxide Aggregate Setting in the Presence of Interstitial Fluid Alone
Section snippets
Materials and Methods
Approval for this project was granted by the Human Research Ethics Committee of the University of Melbourne, Melbourne, Australia. Fifty-four single-rooted teeth were collected from the Royal Dental Hospital of Melbourne tooth bank and were radiographed in the buccolingual and mesiodistal planes to confirm the absence of more than 1 canal, resorption, caries, root fractures, calcifications, or anomalies of the pulp 11, 12. The teeth were stored in formaldehyde (11) before commencement of the
Results
The results of hardness for the different experimental groups as measured by the Vickers hardness test are presented in Table 1 and Figure 2. The greatest mean surface hardness value (VHN = 80.7 ± 35.6) was observed in the group with conditions reflecting the current recommended protocol for the use of MTA (ie, the placement of the wet cotton pellet but no varnish). This was only marginally greater than the main test group with no varnish and no moist cotton pellet (VHN = 78.3 ± 53.7). A 1-way
Discussion
This study indicates that applying a moist cotton pellet to the top of the setting MTA may be an unnecessary extra step and supports recent findings that the restoration of the tooth may proceed without needing the MTA to set (10). This result can be extrapolated to other clinical situations in which the apical surface of the MTA is exposed to tissue fluids and is at least 1 mm in diameter, such as root or pulp chamber perforations. The actual relationship between the diameter and setting was
Conclusions
There was no significant difference between the current protocol group with moist cotton pellet placement and the test group. From this and previously published data 6, 11, it can be concluded that moist cotton pellet placement over MTA in apexification procedures or perforations greater than 1 mm may not be required to aid in the setting of MTA.
Acknowledgments
The authors would like to thank The Melbourne Dental School; Mr Ilya Zalizniak, Biomaterials/Biomechanics Technical Assistant, The University of Melbourne; Mr Mario Smith, Preclinical Laboratory, The University of Melbourne; and Associate Professor Ian Gordon of the Statistical Consulting Centre, University of Melbourne, for their generous support.
The authors deny any conflicts of interest related to this study.
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The flow behavior and sealing ability of calcium silicate root canal cement containing dimethyl sulfoxide: An in vitro study
2024, Journal of the Mechanical Behavior of Biomedical MaterialsBleaching of a Discolored Tooth with Retrieval of Remnants after Successful Regenerative Endodontics
2018, Journal of EndodonticsCitation Excerpt :Similar to another report (38), the MTA barrier was removed before the placement of the bleaching agent. The set MTA adds support to the existing evidence and advice that a moist cotton pellet is not necessary for the setting of MTA (7, 8, 39). A recent in vitro study (40) showed continued release of hydrogen peroxide from a mixture of sodium perborate and water for up to 28 days.
Assessment of mineral trioxide aggregate setting in simulated root canal with different root canal wall thickness: In vitro study
2021, Applied Sciences (Switzerland)Radicular perforation repair with mineral trioxide aggregate: A case report with 10-year follow-up
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Supported by Gunz Dental (Australia), Dentsply (Australia) Research Fund, Dental Health Services Victoria Research and Innovation Grant, and The Melbourne Dental School.