Basic ResearchAntibiofilm Activity of Sodium Hypochlorite and Alkaline Tetrasodium EDTA Solutions
Section snippets
Bacteria Strain and Irrigating Solutions
The bacteria used in this study were E. faecalis American Type Culture Collection 29212, taken from a 4°C stock culture and streaked out twice on brain-heart infusion (BHI) (Scharlau Chemie SA, Barcelona, Spain) agar plates for 24 hours at 37°C. From the subculture of E. faecalis, a 1 McFarland standard suspension was prepared in BHI broth and then diluted 30-fold to obtain an initial bacterial suspension of 1 × 107 colony-forming units (CFUs) per milliliter.
The solutions tested were 2.5% NaOCl
Results
All negative controls showed no bacterial growth. The linear determination coefficient between RLUs and log10 (CFUs + 1) in the groups with positive growth was r2 = 72.8% (r = 0.853) with a P value < .001. Table 1 gives results for luminescence and CFUs. In the NaOCl groups there was no bacterial growth. The ATP readings are consistent with these findings. A similar reduction of RLUs was seen in all NaOCl groups, giving statistically significant differences with respect to the control.
The
Discussion
Culturing techniques are commonly applied to evaluate the antibiofilm efficacy of irrigating solutions in endodontics. The ATP determination may be considered an adequate alternative because it is a rapid means of quantifying bacteria that are viable, even though they do not have the capacity to grow in traditional culture media, hence viable but non-culturable state (17). The bioluminescence ATP assay has sufficient sensitivity in bacteria detection (between 10 and 100 cells) in root canal
Acknowledgments
The authors thank Francisca Castillo Pérez and Gertrudis Gomez Villaescusa for their technical assistance.
This study was supported by the Research Group CTS-167 of the Junta de Andalucía, Spain. This study has been possible by a research initiation fellowship of Plan Propio de investigación de la Universidad de Granada-2016, granted to Carmen Solana, the first author of this paper.
The authors deny any conflicts of interest related to this study.
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