Basic ResearchAntibiofilm Activity of Diclofenac and Antibiotic Solutions in Endodontic Therapy
Section snippets
Materials and Methods
This protocol was approved by the Ethics Committee of the University of Granada, Granada, Spain (no. 1076 CEIH/2020).
Results
The results in terms of CFU reduction percentage ranged between 62.98 and 98.62 for TAS and 5% DCS, respectively. There were no significant differences between the 2 antibiotic solutions (TAS and DAS), whereas the DCS showed a concentration-dependent effect.
For the antimicrobial test with confocal laser scanning microscopy, a total of 120 operative fields (3-dimensional stacks) were evaluated. The log10 total biovolume in all groups showed a scarce (1.39–1.02) but significant reduction with
Discussion
The persistence of microorganisms that resist disinfection procedures and/or the recontamination of the root canal system can prove determinantal for the healing of teeth with periapical periodontitis24,25.
The emergence of antibiotic resistance is a major concern, which has led to the search for new alternative approaches to disinfection of the root canal, including the use of NSAIDs. In this context, we tested the antimicrobial activity of DCSs and compared them with DASs and TASs to evaluate
CRediT authorship contribution statement
Carmen María Ferrer-Luque: Conceptualization, Methodology, Writing - review & editing. Pilar Baca: Formal analysis, Supervision. Carmen Solana: Investigation, Writing - original draft. Alberto Rodríguez-Archilla: Software, Validation. María Teresa Arias-Moliz: Software, Validation. Matilde Ruiz-Linares: Conceptualization, Methodology, Writing- review & editing.
Acknowledgments
The authors thank Francisca Castillo Pérez, Gertrudis Gomez Villaescusa, and Ana Santos Carro for their technical assistance.
Supported by Research Group CTS-167 of the Junta de Andalucía, Seville, Spain.
The authors deny any conflicts of interest related to this study.
References (37)
- et al.
Chemomechanical reduction of the bacterial population in the root canal after instrumentation and irrigation with 1%, 2.5%, and 5.25% sodium hypochlorite
J Endod
(2000) - et al.
Paradigm lost: a perspective on the design and interpretation of regenerative endodontic research
J Endod
(2014) - et al.
Regenerative endodontic therapy: a data analysis of clinical protocols
J Endod
(2015) - et al.
Translational science in disinfection for regenerative endodontics
J Endod
(2014) - et al.
Disinfection of immature teeth with a triple antibiotic paste
J Endod
(2005) - et al.
Regenerative endodontic therapy in the management of nonvital immature permanent teeth: a systematic review-outcome evaluation and meta-analysis
J Endod
(2017) - et al.
Regenerative endodontic procedures in necrotic mature teeth with periapical radiolucencies: a preliminary randomized clinical study
J Endod
(2019) - et al.
A review of tooth discoloration after regenerative endodontic therapy
J Endod
(2016) - et al.
Treatment options: biological basis of regenerative endodontic procedures
J Endod
(2013) - et al.
The antibacterial action of diclofenac shown by inhibition of DNA syn-thesis
Int J Antimicrob Agents
(2000)
Potential management of resistant microbial infections with a novel non-antibiotic: the anti-inflammatory drug diclofenac sodium
Int J Antimicrob Agents
Activity of diclofenac used alone and in combination with streptomycin against Mycobacterium tuberculosis in mice
Int J Antimicrob Agents
Effect of the association of nonsteroidal anti-inflammatory and antibiotic drugs on antibiofilm activity and pH of calcium hydroxide pastes
J Endod
Infection control in teeth with apical periodontitis using a triple antibiotic solution or calcium hydroxide with chlorhexidine: a randomized clinical trial
J Endod
Antibiofilm activity of sodium hypochlorite and alkaline tetrasodium EDTA solutions
J Endod
The effects of antimicrobials on endodontic biofilm bacteria
J Endod
Effect of antimicrobials used in regenerative endodontic procedures on 3-week-old Enterococcus faecalis Biofilm
J Endod
Bacterial quantification in teeth with apical periodontitis related to instrumentation and different intracanal medications: a randomized clinical trial
J Endod
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