Assessment of Laminin-5 in Oral Dysplasia and Squamous Cell Carcinoma

doi:10.1016/j.joms.2013.04.032

Journal of Oral and Maxillofacial Surgery

Volume 71, Issue 11, November 2013, Pages 1873-1879

https://doi.org/10.1016/j.joms.2013.04.032 Get rights and content

Purpose

Laminin-5 is an important protein in the establishment of an intact basement membrane. The aims of this study were to assess the expression of laminin-5 (γ2 chain) using cyclin D1 and Ki-67 in hyperplastic oral mucosal lesions, oral dysplasia, and squamous cell carcinoma (SCC).

Materials and Methods

Paraffin-embedded tissue blocks of 134 patients were stained for laminin-5, cyclin D1, and Ki-67 using immunohistochemistry and assessed by virtual microscopy. Statistical analysis was performed using Kruskal-Wallis tests and Mann-Whitney U tests for post hoc assessment.

Results

Laminin-5, cyclin D1, and Ki-67 were found to have significant differences in expression for the different categories of dysplasia, SCC, and hyperplasia (P < .001). Cyclin D1 and Ki-67 expression levels were significantly increased in moderate and severe dysplasia and SCC, with no significant difference in expression between hyperplasia and mild dysplasia or between biopsies of severe dysplasia and SCC. Laminin-5 expression was only significantly increased in SCC, confirming it as a marker of malignant transformation and invasion.

Conclusions

The results of this study indicate that overexpression of laminin-5 is found only in SCC and not dysplastic lesions. Therefore, laminin-5 has potential as a marker for the intraoperative assessment of cancer excision margins and could be used as a target for chemotherapeutic agents.

Section snippets

Materials and Methods

Tissue blocks of patients with a confirmed diagnosis of mild, moderate, or severe dysplasia, SCC, and tissue hyperplasia of the oral mucosa were obtained from the Oral Pathology Diagnostic Service, Melbourne Dental School, University of Melbourne (Melbourne, Australia) from 1992 through 2009. Data collected included gender and date of birth, with the histologic diagnosis being confirmed by a review of the original slides stained with hematoxylin and eosin by an oral and maxillofacial

Results

One hundred thirty-six patients were included in the study (70 women, 51.5%; 66 men, 48.5%). The mean age of the patient group was 63.1 years (standard deviation, 13.6 yr; range, 30 to 87 yr). The pathologic diagnoses were epithelial hyperplasia in 24 patients (17.6%), mild dysplasia in 27 (19.9%), moderate dysplasia in 8 (5.9%), severe dysplasia in 29 (21.1%), and SCC in 48 (35.3%).

The immunohistochemical results showed significant variation in the mean and median pixel levels for hyperplasia,

Discussion

The present study found a significant increase in cytoplasmic laminin-5 expression in SCC with no significant increase in expression in dysplastic lesions. The expression of cycle markers cyclin D1 and Ki-67 significantly increased with increasing grade of dysplasia and SCC. This is in keeping with the idea that one of the main features of malignant transformation is increased and dysregulated cellular proliferation.

With regard to laminin-5, the findings of the present study add to previous

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PathologyAssessment of Laminin-5 in Oral Dysplasia and Squamous Cell Carcinoma

Purpose

Materials and Methods

Results

Conclusions

Section snippets

Materials and Methods

Results

Discussion

J Oral Maxillofac Surg

Oral Oncol

Oral Oncol

Br J Oral Maxillofac Surg

Arch Oral Biol

Oral Oncol

Br J Oral Maxillofac Surg

Oral Oncol

Oral Surg Oral Med Oral Pathol

Int J Oral Maxillofac Surg

Int J Oral Maxillofac Surg

J Oral Maxillofac Surg

Definition of leukoplakia and related lesions: An aid to studies on oral precancer

Oral Surg Oral Med Oral Pathol

Oral leukoplakia and malignant transformation: A follow-up study of 257 patients

Cancer

An immunohistochemical analysis of cell cycle marker in oral mucosal dysplastic lesions treated by laser therapy: A pilot study

J Maxillofac Oral Surg

Molecular markers in the surgical margin of oral carcinomas

J Oral Pathol Med

Pathology
Assessment of Laminin-5 in Oral Dysplasia and Squamous Cell Carcinoma