ReviewCholesterol involvement in the pathogenesis of neurodegenerative diseases
Introduction
Cholesterol plays integral roles in cell structure and function. It is an essential component of the cell membranes required for membrane lipid organization. Different concentrations of cholesterol regulate membrane fluidity, and thereby structural integrity and functional specificity at various cellular locations. However, cholesterol moves within and between different membranes and intracellular organelles. Cytoplasmic cholesterol is also a source of bioactive molecules such as steroid hormones, vitamin D and bile acids. Thus, cholesterol is implicated in regulating diverse cellular metabolisms, compartmental homeostasis, and molecular interactions in extracellular and intracellular communication. As a polar lipid cholesterol is also toxic to its host cell, and when accumulated, it causes cell death. It is thus important that while cholesterol is required, it must be harnessed in certain forms, locations and concentrations. Extensive studies on cholesterol synthesis have been carried out in liver and vascular endothelial cells. In contrast, much remains to be understood about the role of cholesterol in normal brain function, and how the brain cells deal with high levels of cholesterol under various conditions including aging. Recently considerable evidence implicates cholesterol in the loss of nerve cells e.g. as demonstrated in the cholesterol transport disorder of Niemann–Pick disease type C (NPC). In this review, we discuss the general aspect of cholesterol distribution and function in the brain, and recent advances in our understanding of cholesterol involvement in Alzheimer's disease (AD), Parkinson's disease (PD) and NPC.
Section snippets
Cholesterol distribution and function in the brain
Brain contains the highest levels of cholesterol in mammalian bodies. The human brain contains as much as 25% of total body cholesterol and cholesterol derivatives (∼ 20 mg/g), although the human brain accounts for ∼ 2% of total body weight (Dietschy and Turley, 2004, Vaya and Schipper, 2007). In the mouse, the brain contains about 15% of total cholesterol (15 mg/g), from ∼ 8 mg/g in the grey matter to ∼ 40 mg/g in the spinal cord (Xie et al., 2003). The five-fold more cholesterol in the spinal
Cholesterol in Alzheimer's disease
Recent studies suggest that high-level cholesterol is a risk factor in CNS pathophysiology associated with the development of neurodegenerative diseases such as Alzheimer's disease (AD) and Parkinson's disease (PD) (reviewed in Simons and Ehehalt (2002) and Wustner et al. (2005)). Systematic meta-analyses of Alzheimer disease genetic association suggest that SOAT1 (the gene encoding Acyl-coenzyme A:cholesterol acyltransferase or ACAT) is associated with risk of AD and that a common polymorphism
Cholesterol in Parkinson's disease
It has been shown that body mass index is associated with risk of Parkinson's disease in a retrospective study of 272 men and 254 women with PD from study cohorts of 22,367 Finnish men and 23,439 women in 18.8 years (Hu et al., 2006). In another large prospective analysis, high dietary intake of cholesterol increases the risk of PD (Hu et al., 2008). At cellular and molecular levels, oxidative stress, inflammation and increased α-synuclein confer risk for development of
Niemann–Pick disease type C
Niemann–Pick disease encompasses a heterogeneous group of lysosomal lipid storage diseases with autosomal-recessive inheritance. Patients with type A have severe neural and visceral symptoms, and patients with type B have chronic visceral symptoms, due to mutations of sphingomyelinase and accumulation of acidic sphingomyelin in lysosomes. NPC is a subacute progressive neurodegenerative disorder, with cholesterol and sphingolipid accumulation in endosomes and lysosomes of neurons due to
Other function and interface of NPC1 and NPC2 pathway
Studies show that there is a defect in NPC neurons of cholesterol trafficking from the endogenous synthetic pathway to axons through the Golgi apparatus, but not from the plasma membrane endocytic pathway (Karten et al., 2003). Recently, NPC1 has been shown to reduce accumulation of axonal spheroids (Zhang et al., 2008). Additional to neuronal defects, there is also a loss of fibrillary astrocyte function in NPC, but enforced expression of NPC1 results in an astrocyte-specific rescue of the
Concluding remarks
Cholesterol transport in the brain involves trafficking between intracellular organelles in and between neurons and glial cells. Deregulation of cholesterol transport with increased cholesterol deposition in neurons is implicated not only in NPC but also AD and PD. α-Synuclein interacts with cholesterol rafts in its transport to nerve terminals, and the excess protein–lipid complex is a hallmark of PD. NPC1 and NPC2 may coordinate to transfer cholesterol from each other, while NPC1 is also
Acknowledgments
This work was supported by grants from the National Health and Medical Research Council of Australia, and Cancer Council of Victoria, Australia. Y.T. is a scholarship recipient of the Chinese Scholarship Council.
References (119)
- et al.
ATP-binding cassette transporter A1 (ABCA1) deficiency does not attenuate the brain-to-blood efflux transport of human amyloid-beta peptide (1–40) at the blood–brain barrier
Neurochem. Int.
(2008) - et al.
Evolution of the vertebrate ABC gene family: analysis of gene birth and death
Genomics
(2006) - et al.
Cholesterol level regulates endosome motility via Rab proteins
Biophys. J.
(2008) - et al.
Synthesis and biochemical properties of a new photoactivatable cholesterol analog 7,7-azocholestanol and its linoleate ester in Chinese hamster ovary cell lines
J. Lipid. Res.
(2002) Phospholipid flippases
J. Biol. Chem.
(2007)- et al.
Topological analysis of Niemann–Pick C1 protein reveals that the membrane orientation of the putative sterol-sensing domain is identical to those of 3-hydroxy-3-methylglutaryl-CoA reductase and sterol regulatory element binding protein cleavage-activating protein
J. Biol. Chem.
(2000) - et al.
Topological analysis of Niemann–Pick C1 protein reveals that the membrane orientation of the putative sterol-sensing domain is identical to those of 3-hydroxy-3-methylglutaryl-CoA reductase and sterol regulatory element binding protein cleavage-activating protein
J. Biol. Chem.
(2000) - et al.
The human ATP-binding cassette (ABC) transporter superfamily
J. Lipid. Res.
(2001) - et al.
Thematic review series: brain lipids. Cholesterol metabolism in the central nervous system during early development and in the mature animal
J. Lipid. Res.
(2004) - et al.
NPC1 and NPC2 regulate cellular cholesterol homeostasis through generation of low density lipoprotein cholesterol-derived oxysterols
J. Biol. Chem.
(2003)
Cholesterol accumulation sequesters Rab9 and disrupts late endosome function in NPC1-deficient cells
J. Biol. Chem.
Glial lipoproteins stimulate axon growth of central nervous system neurons in compartmented cultures
J. Biol. Chem.
Niemann–Pick C1 is a late endosome-resident protein that transiently associates with lysosomes and the trans-Golgi network
Mol. Genet. Metab.
Deficiency of ABCA1 impairs apolipoprotein E metabolism in brain
J. Biol. Chem.
The absence of ABCA1 decreases soluble ApoE levels but does not diminish amyloid deposition in two murine models of Alzheimer disease
J. Biol. Chem.
The ACAT inhibitor CP-113,818 markedly reduces amyloid pathology in a mouse model of Alzheimer's disease
Neuron
Purified NPC1 Protein: I. Binding of cholesterol and oxysterols to a 1278-amino acid membrane protein
J. Biol. Chem.
Purified NPC1 Protein: II. Localization of sterol binding to a 240-amino acid soluble luminal loop
J. Biol. Chem.
Regulation of beta-amyloid levels in the brain of cholesterol-fed rabbit, a model system for sporadic Alzheimer's disease
Mech. Ageing. Dev.
ApoE promotes the proteolytic degradation of Abeta
Neuron
Binding of alpha-synuclein affects the lipid packing in bilayers of small vesicles
J. Biol. Chem.
Trafficking of cholesterol from cell bodies to distal axons in Niemann Pick C1-deficient neurons
J. Biol. Chem.
Expression of ABCG1, but not ABCA1, correlates with cholesterol release by cerebellar astroglia
J. Biol. Chem.
Role of ABCG1 and ABCA1 in regulation of neuronal cholesterol efflux to apolipoprotein E discs and suppression of amyloid-beta peptide generation
J. Biol. Chem.
. Cholesterol-mediated neurite outgrowth is differently regulated between cortical and hippocampal neurons
J. Biol. Chem.
Lack of ABCA1 considerably decreases brain ApoE level and increases amyloid deposition in APP23 mice
J. Biol. Chem.
Cholesterol accumulation is associated with lysosomal dysfunction and autophagic stress in Npc1−/− mouse brain
Am. J. Pathol.
NPC2, the protein deficient in Niemann–Pick C2 disease, consists of multiple glycoforms that bind a variety of sterols
J. Biol. Chem.
Protein kinase C and its substrates
Mol.Cell.Endocrinol.
. Knockout of the cholesterol 24-hydroxylase gene in mice reveals a brain-specific mechanism of cholesterol turnover
J. Biol. Chem.
Oxidation of cholesterol by amyloid precursor protein and beta-amyloid peptide
J. Biol. Chem.
Before the loss: neuronal dysfunction in Niemann–Pick Type C disease
Biochim. Biophys. Acta
Apolipoprotein E polymorphism and Alzheimer's disease
Lancet
Role of cholesterol in the function and organization of G-protein coupled receptors
Prog. Lipid Res.
Ontogenesis and regulation of cholesterol metabolism in the central nervous system of the mouse
Brain Res. Dev. Brain Res.
Direct binding of cholesterol to the purified membrane region of SCAP: mechanism for a sterol-sensing domain
Mol. Cell
Association studies of cholesterol metabolism genes (CH25H, ABCA1 and CH24H) in Alzheimer's disease
Neurosci. Lett.
Cholesterol in Alzheimer's disease
Lancet. Neurol.
Expression of liver × receptor target genes decreases cellular amyloid beta peptide secretion
J. Biol. Chem.
Lipid imbalance in the neurological disorder, Niemann–Pick C disease
FEBS. Lett.
Effects of the cholesterol-lowering compound methyl-beta-cyclodextrin in models of alpha-synucleinopathy
J. Neurochem.
Statins reduce neuronal alpha-synuclein aggregation in in vitro models of Parkinson's disease
J. Neurochem.
Visualization of Rab9-mediated vesicle transport from endosomes to the trans-Golgi in living cells
J. Cell. Biol.
Brain neutral lipids mass is increased in alpha-synuclein gene-ablated mice
J. Neurochem.
Cholesterol accumulation and liver cell death in mice with Niemann–Pick type C disease
Hepatology
Systematic meta-analyses of Alzheimer disease genetic association studies: the AlzGene database
Nat. Genet.
Crossing the barrier: oxysterols as cholesterol transporters and metabolic modulators in the brain
J. Int. Med.
Brain cholesterol: long secret life behind a barrier
Arterioscler. Thromb. Vasc. Biol.
Defective endocytic trafficking of NPC1 and NPC2 underlying infantile Niemann–Pick type C disease
Hum. Mol. Genet.
Elevated levels of oxidized cholesterol metabolites in Lewy body disease brains accelerate alpha-synuclein fibrilization
Nat. Chem. Biol.
Cited by (166)
Hepatic cholesterol biosynthesis and dioxin-induced dysregulation: A multiscale computational approach
2023, Food and Chemical ToxicologyThe Endo-lysosomal System in Parkinson's Disease: Expanding the Horizon
2023, Journal of Molecular BiologyThe synaptic lipidome in health and disease
2022, Biochimica et Biophysica Acta - BiomembranesStructure-based virtual screening for identification of potential non-steroidal LXR modulators against neurodegenerative conditions
2022, Journal of Steroid Biochemistry and Molecular BiologySynthesis and biological evaluation of cationic TopFluor cholesterol analogues
2021, Bioorganic Chemistry