Human Dental Pulp Stem Cells. A promising epithelial-like cell source
Section snippets
Background
Human Dental Pulp Stem Cells (HDPSC) have inherent differentiation capabilities and relatively high proliferation rate and self-renewal capability as compared with other adult mesenchymal stem cells [1]. Moreover, HDPSC are characterized by the expression of typical markers of undifferentiated cells such as STRO-1, Oct-4, Sox2, CD90, CD105 [2], and this may explain the enormous potential of these cells to differentiate into multiple mesodermal cell lineages, including, among others,
Hypothesis
Human Dental Pulp Stem Cells (HDPSC) have the potential to differentiate into multiple cell lineages by the appropriate differentiation conditions, but skin epidermis differentiation has not been demonstrated so far. We hypothesize that HDPSC may have pluripotent differentiation capability, including the potential to differentiate into skin epithelial keratinocytes in culture. To fully induce the epidermal differentiation of these cells, co-culture of HDPSC with dermal fibroblasts should be
Conclusion
Several pathologies compromise the normal skin, including extense full-thickness burns leading to severe dysfunction and mortality that demand a rapid and major treatment. The therapy of severe burns has substantially improved the outcome of these patients in the last decade. However, the lack of healthy skin donor sites and the delayed time that is necessary to obtain large keratinocytes cultures represent a challenge when rapid skin coverage is needed [16]. The availability of a novel
Conflict of interest
None declared.
Acknowledgements
This work was supported by the University of Granada CEIBiotic Grant CEI2014-MPBS14 and the Spanish Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I+D+I) from the National Ministry of Economy and Competitiveness (Instituto de Salud Carlos III), Grant FIS PI11-1582 (co-financed by FEDER funds, European Union).
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