Differential gene expression in the developing mouse ureter
Section snippets
Results and discussion
The mouse ureter develops from the ureteric bud (UB) which is an epithelial outgrowth of the Wolffian duct (WD) at embryonic day (E) 10.5. The site at which the UB emerges from the WD is well orchestrated such that it will invade a mass of metanephric mesenchyme (MM) which will become the permanent kidney (metanephros). Reciprocal inductive signals between the UB and MM induces branching of the UB which develops into the collecting duct and calyceal system of the kidney and the induction of
Conclusion
This study constitutes the first report defining global gene expression in the developing mouse ureter. The known and novel genes we have identified provide a valuable catalogue of candidate genes to investigators studying the morphogenesis of the ureter and the molecular mechanisms underlying ureter abnormalities. Several genes known to play critical roles in ureter development and associated with ureter and kidney anomalies were identified in this screen such as foxc1, foxc2, UPIIIa and the
Animals for histology
B6×CBA females were time-mated. Pregnant animals were sacrificed using cervical dislocation and embryos collected into phosphate buffered saline (PBS) and immediately decapitated. Ureters with attached metanephroi and Wolffian Duct were dissected at embryonic day (E) 12.5. Ureters with attached metanephroi and bladders were dissected at E15.5 and postnatal day (P) 75. Urinary tracts were fixed in 4% paraformaldehyde/PBS overnight for histology.
Histology
Tissue was transferred from 4% paraformaldehyde to
Acknowledgements
This work was performed as part of the Renal Regeneration Consortium. We would like to thank Ms. Elizabeth O'Brien and Ms. Bree Rumballe for supplying cDNA clones obtained from the SRC Microarray Facility, Institute for Molecular Bioscience, The University of Queensland. E.M is a recipient of an Australian Postgraduate Scholarship. This work was supported by the National Institute for Diabetes, Digestion and Kidney Disease, National Institutes of Health (DK63400) as part of the Stem Cell Genome
References (58)
- et al.
GFRalpha1 is an essential receptor component for GDNF in the developing nervous system and kidney
Neuron
(1998) - et al.
Murine Wnt-11 and Wnt-12 have temporally and spatially restricted expression patterns during embryonic development
Mech. Dev.
(1995) - et al.
Metabolic deficiencies in alcohol dehydrogenase Adh1, Adh3, and Adh4 null mutant mice. Overlapping roles of Adh1 and Adh4 in ethanol clearance and metabolism of retinol to retinoic acid
J. Biol. Chem.
(1999) - et al.
GFR alpha1-deficient mice have deficits in the enteric nervous system and kidneys
Neuron
(1998) - et al.
Genomic organization and expression of KCNJ8/Kir6.1, a gene encoding a subunit an ATP-sensitive potassium channel
Gene
(1998) - et al.
Genomic organisation, alternative splicing and polymorphisms of the human cardiac troponin T gene
J. Mol. Cell. Cardiol.
(1998) The biology of the small leucine-rich proteoglycans. Functional network of interactive proteins
J. Biol. Chem.
(1999)- et al.
Characterization of mouse thrombospondin 2 sequence and expression during cell growth and development
J. Biol. Chem.
(1992) - et al.
Duplex kidneys: a correlation of renal dysplasia with position of the ureteral orifice
J. Urol.
(1975) - et al.
Evidence that bone morphogenetic protein 4 has multiple biological functions during kidney and urinary tract development
Kidney Int.
(2003)