Efficacy of a murine-p75-saporin immunotoxin for selective lesions of basal forebrain cholinergic neurons in mice
Section snippets
Acknowledgements
The immunotoxin was provided generously by Dr. Douglas Lappi, Advanced Targeting Systems. We would like to express our thanks to Ms. Patience Carey and Ms. Ginny Quinan for their excellent care of our animals. This project was supported by NIH 5R44-NS034591.
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Long-term effects of selective immunolesions of cholinergic neurons of the nucleus basalis magnocellularis on the ascending cholinergic pathways in the rat: A model for Alzheimer's disease
2013, Brain Research BulletinCitation Excerpt :Its specificity makes 192 IgG-saporin a useful agent with which to establish specific cholinergic lesions modeling the AD-associated cholinergic hypofunction. Although deficits in cholinergic function following several types of lesions to the nBM are well documented (Harati et al., 2008; Nag et al., 2009; Pizzo et al., 1999), there have been only a few reports regarding the time course of the development of this cholinergic hypofunction or its potential recovery (Abdulla et al., 1997; Höhmann and Coyle, 1988; Perry et al., 2001; Rossner et al., 1995a,b). Our present goal was therefore to demonstrate the persistence of a 192 IgG-saporin-induced lesion in the nBM and the long-term cortical response to this selective cholinergic neuron loss in architectonically well-defined frontal and parietal cortical projection areas.
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2011, Behavioural Brain ResearchCitation Excerpt :Because the use of a selective cholinergic lesion would be interesting for studying cholinergic deficits in transgenic mice, a mouse variant of the immunotoxin saporin was developed. Despite some initial problems, a mu p75-saporin toxin was developed that could be infused intracerebroventricularly [32,33]. In mice mu p75-saporin lesions were also reported to impair learning and memory functions which could not be attributed to lesions of cerebellar Purkinje cells.
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