Elsevier

Neuroscience Letters

Volume 458, Issue 1, 10 July 2009, Pages 32-36
Neuroscience Letters

P2X receptors are expressed on neurons containing luteinizing hormone-releasing hormone in the mouse hypothalamus

https://doi.org/10.1016/j.neulet.2009.04.017Get rights and content

Abstract

Expression of P2X1, P2X2, P2X3, P2X4, P2X5 and P2X6 receptors, members of a family of ATP-gated cation channels, on neurons containing luteinizing hormone-releasing hormone (LHRH) in the mouse hypothalamus was studied with double-labeling fluorescence immunohistochemistry. This study demonstrated that different combinations of P2X receptor subunits were expressed on the perikarya and axon terminals of LHRH-producing neurons. It was shown for the first time that P2X2, P2X4, P2X5 and P2X6 receptor subunits were expressed on the perikarya of LHRH-producing neurons and P2X2 and P2X6 on their axon terminals. These results suggest that activation of P2X receptors by ATP via different homomeric or heteromeric P2X receptors at both presynaptic and postsynaptic sites could be involved in the regulation of LHRH secretion at the forebrain level.

Section snippets

Acknowledgments

The authors thank Dr. Gillian E. Knight for her excellent editorial assistance. We also thank Roche Bioscience, Palo Alto, CA for kindly supplying P2X antisera and peptides. This work was supported by the National Natural Science Foundation of PR China (30670639 to Z. Xiang).

References (41)

  • A.J. Silverman et al.

    Quantitative analysis of synaptic input to gonadotropin-releasing hormone neurons in normal mice and hpg mice with preoptic area grafts

    Brain Res.

    (1988)
  • N. Teramoto et al.

    Simultaneous detection of two independent antigens by double staining with two mouse monoclonal antibodies

    J. Virol. Methods

    (1998)
  • Y. Tsuruo et al.

    Morphological evidence for neuronal regulation of luteinizing hormone-releasing hormone-containing neurons by neuropeptide Y in the rat septo-preoptic area

    Neurosci. Lett.

    (1990)
  • G.F. Turi et al.

    Cholinergic afferents to gonadotropin-releasing hormone neurons of the rat

    Neurochem. Int.

    (2008)
  • Z. Xiang et al.

    P2X5 receptors are expressed on neurons containing arginine vasopressin and nitric oxide synthase in the rat hypothalamus

    Brain Res.

    (2006)
  • P. Bodin et al.

    Purinergic signalling: ATP release

    Neurochem. Res.

    (2001)
  • G. Burnstock

    Physiology and pathophysiology of purinergic neurotransmission

    Physiol. Rev.

    (2007)
  • G. Burnstock

    Purine and pyrimidine receptors

    Cell. Mol. Life Sci.

    (2007)
  • G. Burnstock

    Purinergic cotransmission

    Exp. Physiol.

    (2009)
  • G.H. Burrows et al.

    Comparison of the effects of ATP, Mg2+, and MgATP on the release of luteinizing hormone-releasing hormone from isolated hypothalamic granules

    J. Neurochem.

    (1982)
  • Cited by (14)

    • Purinergic signaling pathways in endocrine system

      2015, Autonomic Neuroscience: Basic and Clinical
      Citation Excerpt :

      P2X2 and P2X4 subunits are expressed in monkey GnRH neurons (Terasawa et al., 2005) and this was confirmed in mouse and rat by immunohistochemical analysis done in Burnstock's lab (Loesch and Burnstock, 2001; Loesch et al., 1999; Xiang et al., 1998). They also found the presence of P2X5R and P2X6R immunoreactivity in mouse GnRH neurons (Fu et al., 2009). Transgenic mice and rats expressing GFP-labeled GnRH neurons provide a promising system to investigate the expression and role of purinergic receptors in GnRH neurons more directly.

    • P2X2 purinoreceptor protein in hypothalamic neurons associated with the regulation of food intake

      2010, Neuroscience
      Citation Excerpt :

      The chemical phenotypes of P2X2Rs in the hypothalamus have been investigated earlier. P2X2R protein has been demonstrated in neurons containing luteinizing hormone-releasing hormone (LHRH) in the area around the organum vasculosum of lamina terminals (OVLT) (Fu et al., 2009), and in vasopressin and oxytocin neurons of the supraoptic nucleus (Guo et al., 2009). P2X2Rs have also been observed in neurons of the supraoptic nucleus containing NOS (Yao et al., 2003).

    View all citing articles on Scopus
    1

    These two authors contributed equally to this work.

    View full text