Expression and localization of the Parkin Co-Regulated Gene in mouse CNS suggests a role in ependymal cilia function
Section snippets
Supplementary data
Supplement 1: Widespread expression of Pacrg. Pacrg transcript was detected in the cerebral cortex (A), hippocampus (B) and the cerebellum (C). Adjacent sections analysed with the sense control probe were negative for Pacrg transcript staining (D–F). Orientation of all sections is marked by dorsal (d) and caudal (c) in the cerebral cortex (A). Calibration bar 100 μm.
Supplement 2: Pacrg is highly expressed in divergent CNS nuclei. Pacrg transcript was detected in the oculomotor nucleus (A),
Acknowledgements
The authors acknowledge the assistance of laboratory and animal facility staff. This work was supported in part by National Health & Medical Research Council (Australia) project grants 334349 and 436977 to PJL. GRW is an NHMRC Dora Lush Scholar (384489), PJL is an NHMRC RD Wright Fellow (334346) and MBD is an NHMRC Practitioner Fellow (284520).
References (21)
- et al.
Regional and cellular localisation of Parkin Co-Regulated Gene in developing and adult mouse brain
Brain Res.
(2008) Parkin-co-regulated gene (PACRG) product interacts with tubulin and microtubules
FEBS Lett.
(2008)- et al.
A product of the human gene adjacent to Parkin is a component of Lewy bodies and suppresses Pael receptor-induced cell death
J. Biol. Chem.
(2003) - et al.
Proteomic analysis of isolated chlamydomonas centrioles reveals orthologs of ciliary-disease genes
Curr. Biol.
(2005) - et al.
Parkin localizes to the Lewy bodies of Parkinson disease and dementia with Lewy bodies
Am. J. Pathol.
(2002) - et al.
Parkin Co-Regulated Gene (PACRG) is regulated by the ubiquitin-proteasomal system and is present in the pathological features of Parkinsonian diseases
Neurobiol. Dis.
(2007) - et al.
Identification of a novel gene linked to Parkin via a bi-directional promoter
J. Mol. Biol.
(2003) - et al.
Centriole/basal body morphogenesis and migration during ciliogenesis in animal cells
J. Cell Sci.
(2007) - et al.
The Parkin co-regulated gene product, PACRG, is an evolutionarily conserved axonemal protein that functions in outer-doublet microtubule morphogenesis
J. Cell Sci.
(2005) - et al.
Differential immunocytochemical staining for glial fibrillary acidic (GFA) protein, S-100 protein and glutamine synthetase in the rat subcommissural organ, nonspecialized ventricular ependyma and adjacent neuropil
Cell Tissue Res.
(1986)
Cited by (15)
Crystal structure of human PACRG in complex with MEIG1 reveals roles in axoneme formation and tubulin binding
2021, StructureCitation Excerpt :Mouse PACRG is highly abundant in testis and is required for spermiogenesis (Lorenzetti et al., 2004). Loss of PACRG results in hydrocephalus in both PACRG KO and qkv mice (Stephenson et al., 2018; Wilson et al., 2009); PACRG levels are indeed elevated in ependymal cells lining the cerebral ventricles, suggesting that PACRG is required for cilium motility. Knockdown of the two PACRG paralogs in trypanosome, a protozoan parasite, results in paralysis of the flagellum and loss of outer doublet microtubules (Dawe et al., 2005).
DNA methylation patterns of protein coding genes and long noncoding RNAs in female schizophrenic patients
2015, European Journal of Medical GeneticsCitation Excerpt :In addition, hypermethylation of PACRG-AS1 (Fig. 6C) was also found in paranoid SCZ. PACRG-AS1 is the antisense gene of PACRG (Parkin Co-Regulated Gene) which shares a bi-directional promoter with PARKIN gene, whose mutations were the most common cause of early-onset autosomal recessive Parkinson's disease [Taylor et al., 2007, 2012; Wilson et al., 2009]. These antisense transcripts may regulate the expression of corresponding protein coding genes and affect the processes of SCZ.
Patched1 haploinsufficiency impairs ependymal cilia function of the quaking viable mice, leading to fatal hydrocephalus
2011, Molecular and Cellular NeuroscienceCitation Excerpt :Defective spermatogenesis in qkv males has been attributed to loss of PACRG resulting in an arrest in the final stage of differentiation due to abnormal axoneme structure (Lorenzetti et al., 2004). PACRG expression has also been observed in the ciliated ependymal cells lining the ventricles of the brain (Wilson et al., 2009). Loss of PACRG expression in the ciliated ependymal cells lining the ventricles of the brain has recently been shown to be responsible for the mild hydrocephalic phenotype observed in qkv mice (Wilson et al., 2010).
Choroid plexus epithelium and its role in neurological diseases
2022, Frontiers in Molecular NeuroscienceEpendymal cells and neurodegenerative disease: outcomes of compromised ependymal barrier function
2022, Brain CommunicationsMotile cilia genetics and cell biology: big results from little mice
2021, Cellular and Molecular Life Sciences