Neuron
Volume 82, Issue 3, 7 May 2014, Pages 659-669
Journal home page for Neuron

Article
Sorting Nexin 27 Regulation of G Protein-Gated Inwardly Rectifying K+ Channels Attenuates In Vivo Cocaine Response

https://doi.org/10.1016/j.neuron.2014.03.011Get rights and content
Under an Elsevier user license
open archive

Highlights

  • DA-specific SNX27−/− mice have reduced GABABR-activated GIRK currents

  • SNX27 deletion reduces neuronal inhibition mediated by GABAB receptors

  • Mice lacking SNX27 in DA neurons are hypersensitive to acute cocaine

  • Expression of SNX27-insensitive GIRK2a restores GIRK currents and cocaine response

Summary

The subcellular pathways that regulate G protein-gated inwardly rectifying potassium (GIRK or Kir3) channels are important for controlling the excitability of neurons. Sorting nexin 27 (SNX27) is a PDZ-containing protein known to bind GIRK2c/GIRK3 channels, but its function in vivo is poorly understood. Here, we investigated the role of SNX27 in regulating GIRK currents in dopamine (DA) neurons of the ventral tegmental area (VTA). Mice lacking SNX27 in DA neurons exhibited reduced GABABR-activated GIRK currents but had normal Ih currents and DA D2R-activated GIRK currents. Expression of GIRK2a, an SNX27-insensitive splice variant, restored GABABR-activated GIRK currents in SNX27-deficient DA neurons. Remarkably, mice with significantly reduced GABABR-activated GIRK currents in only DA neurons were hypersensitive to cocaine and could be restored to a normal locomotor response with GIRK2a expression. These results identify a pathway for regulating excitability of VTA DA neurons, highlighting SNX27 as a promising target for treating addiction.

Cited by (0)