NeuropharmacologyGenetic interdependence of adenosine and dopamine receptors: Evidence from receptor knockout mice
Section snippets
Experimental procedures
All experiments were performed in accordance with the Prevention of Cruelty to Animals Act 1986, under the guidelines of the Australian National Health and Medical Research Council Code of Practice for the Care and Use of Animals for Experimental Purposes in Australia. Formal approval for all experiments was granted by the Department of Pharmacology Animal Ethics Committee (Monash University). All efforts were made to minimize the number of animals used and their suffering, and all experiments
Dopamine D1 receptors
D1 receptor mRNA was detected in the CPu, NAcc and OT in all mice examined other than D1 receptor knockout mice. The addition of a 100-fold excess of unlabeled oligonucleotide completely abolished the hybridization signal. The hybridization signal in D1 receptor knockout mice resembled that of non-specific background. No differences in the level of D1 mRNA were found between D2 or D3 receptor knockout and wild-type mice (data not shown). D1 receptor mRNA was elevated in the CPu and NAcc of A2A
Discussion
While some studies have examined the mRNA expression and binding of various dopamine receptors in mice with specific deletions of dopamine receptor subtypes (e.g. Baik et al 1995, Accili et al 1996, Wong et al 2003), few studies have compared the expression of adenosine receptors in dopamine receptor knockout mice, and vice versa. Moreover, no study has previously interrogated these systems in a complementary and concurrent manner to examine dopamine D1 and D2 receptor expression in adenosine A
Acknowledgments
This work was supported by the National Health and Medical Research Council of Australia (program grant 236805) of which A.J.L. is a Senior Research Fellow and J.D. a Practitioner Fellow.
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