Cellular neuroscienceTransmembrane protein 50b (C21orf4), a candidate for Down syndrome neurophenotypes, encodes an intracellular membrane protein expressed in the rodent brain
Section snippets
Animals
Animals used in the present study were Wistar rats, NMRI or C57BL/6 mice. Pregnant Wistar rats or Wistar pups were used for in vitro studies while male Wistar rats were used for protein extraction for Western blot. All other experiments were performed with C57BL/6 mice with the exception of embryonic in situ hybridization where NMRI mice were used to ensure maximum comparability with large-scale ongoing studies. Experiments were carried out in accordance with institutional guidelines that aim
Tmem50b mRNA expression
In situ hybridization at E14.5 (Fig. 1A–D) demonstrated high Tmem50b mRNA expression in the embryonic nervous system: the cortical plate, trigeminal ganglion, dorsal root ganglia and the spinal cord. Tmem50b is widely expressed in the postnatal brain at P7 (Fig. 1E–I) including the olfactory bulb, the cortex, the hippocampus and the cerebellum as well as many other regions. Expression is observed throughout layers II–VI of the postnatal cortex (Fig. 1F). In the olfactory bulb, expression is
Discussion
The expression patterns of many chromosome 21 (Hsa21) genes have been determined in a previous large-scale in situ hybridization screen which provided cursory evidence for expression of Tmem50b in the embryonic and adult mouse brain (Reymond et al 2002, Gitton et al 2002). During the current experiments, the Allen brain atlas (brain-map.org; Lein et al., 2007) confirmed the cursory results, suggesting that Tmem50b could be a candidate for DS brain phenotypes. In addition, we have previously
Conclusion
In conclusion, Tmem50b is an intracellular membrane protein expressed in key brain regions throughout development with a marked expression in glial cells that may be involved in cellular responses to cytokines.
Acknowledgments
R.X.M. is a CJ Martin Fellow and P.M.B. is a Senior Principal Research Fellow of the NHMRC, Australia. This project was supported by the Fondation Jérôme Lejeune. P.M.B. acknowledges the Joliot Curie Visiting Professorship and the hospitality of ESPCI. The authors wish to acknowledge the assistance of Sharon Mason, DanaKai Bradford, Luce Dauphinot, George Lutfalla, Jean Delabar, Florence Pouradier, Christophe Leterrier, Fabien Pasteau, Yann Herault, Jack-Christophe Cossec, Tania Vitalis and
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Present address: Lawrence Berkeley National Laboratory, Genomics Division, Berkeley, CA 94720, USA.