Short ReportElectrophysiological properties of neurosensory progenitors derived from human embryonic stem cells
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Abbreviations
4-AP
4-aminopyridine
AN
auditory neuron
AP
action potential
APMax
maximum number of action potentials
BDNF
brain derived neurotrophic factor
bFGF
basic fibroblast growth factor
cDNA
complementary deoxyribonucleic acid
DIV
days in vitro
EGF
epidermal growth factor
HCN
hyperpolarization-activated cyclic nucleotide-gated channels
hESC
human embryonic stem cell
HFFs
human foreskin fibroblast feeders
Ih
hyperpolarization-activated current
IK
potassium current
INa
sodium current
K
slope factor
NBM
Neurobasal media
NFM
neurofilament
NS
neurosphere
NT3
neurotrophin 3
NTs
neurotrophins
pps
pulses per second
qRT-PCR
quantitative real time polymerase chain reaction
RIN
input resistance
RS
series resistance
RMP
resting membrane potential
RNA
ribonucleic acid
SEM
standard error of the mean
TEA
tetraethylammonium
TTX
tetrodotoxin
Vh
half-activation voltage
Y27
small peptide Y27632
ZD7288
4-ethylphenylamino-1,2-dimethyl-6-methylaminopyrimidinium chloride
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These authors contributed equally to this study.
Copyright © 2013 Elsevier B.V.