Elsevier

Stem Cell Research

Volume 34, January 2019, 101380
Stem Cell Research

Lab Resource: Multiple Cell Lines
Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults

https://doi.org/10.1016/j.scr.2018.101380Get rights and content
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Abstract: We describe the generation and characterization of 5 human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. The iPSC lines exhibited a normal karyotype, expressed pluripotency markers and differentiated into cells representative of the three embryonic germ layers. These iPSC lines can be used as controls in studying disease mechanisms.

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These authors contributed equally.