Functional Studies of Missense TREM2 Mutations in Human Stem Cell-Derived Microglia

Highlights

• Microglia can be efficiently differentiated from human iPSCs

• iPSC microglia resemble human primary microglia transcriptionally and functionally

• Disease-causing mutations in TREM2 affect receptor processing and expression

• TREM2 mutant microglia differentiate, respond to pathogenic stimuli, and phagocytose

Summary

The derivation of microglia from human stem cells provides systems for understanding microglial biology and enables functional studies of disease-causing mutations. We describe a robust method for the derivation of human microglia from stem cells, which are phenotypically and functionally comparable with primary microglia. We used stem cell-derived microglia to study the consequences of missense mutations in the microglial-expressed protein triggering receptor expressed on myeloid cells 2 (TREM2), which are causal for frontotemporal dementia-like syndrome and Nasu-Hakola disease. We find that mutant TREM2 accumulates in its immature form, does not undergo typical proteolysis, and is not trafficked to the plasma membrane. However, in the absence of plasma membrane TREM2, microglia differentiate normally, respond to stimulation with lipopolysaccharide, and are phagocytically competent. These data indicate that dementia-associated TREM2 mutations have subtle effects on microglia biology, consistent with the adult onset of disease in individuals with these mutations.