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Cellular and humoral responses in liver of cattle and buffaloes infected with a single dose of Fasciola gigantica

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Abstract

The cellular components of the hepatic inflammatory infiltrate in cattle and buffaloes infected with a single dose of 1000 Fasciola gigantica were analysed by immunohistochemistry and histology. T and B lymphocytes, plasma cells, eosinophils and mast cells were present in the hepatic lesions. It is proposed that both cellular and humoral immune responses were induced in the liver of cattle and buffaloes during infection with F. gigantica probably by antigens released by the developing flukes and by damage caused by the flukes during their migration in the liver. The local T cell response differed between these animals, with the response decreasing after 3 weeks post-infection in cattle in contrast to a gradually increasing response in buffaloes. Difference in the T cell response between cattle and buffaloes may be related to their differences in resistance and resilience to infection with F. gigantica.

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Acknowledgments

The study was supported by the Australian Centre for International Agricultural Research (ACIAR), Canberra as part of the project on the Control of Fasciolosis in Indonesia, Cambodia and the Philippines and post-graduate research of the corresponding author. The LRF Immunodiagnostics Unit, Department of Clinical Biochemistry and Cellular Science, University of Oxford, Oxford, U.K. through Prof. David Mason is gratefully acknowledged for providing the anti-human CD79b monoclonal antibody.

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