Maturation and Differentiation of Cultured Fetal Stomach: Effects of Corticosteroids, Pentagastrin, and Cytochalasin B

doi:10.1016/S0016-5085(76)80359-9

Gastroenterology

Volume 71, Issue 5, November 1976, Pages 770-777

https://doi.org/10.1016/S0016-5085(76)80359-9 Get rights and content

Abstract

Gastric mucosa of fetal rats undergoes striking developmental changes during the last few days of gestation in utero. To investigate some aspects of this process, gastric explants from 18-day fetuses (4 days before birth) were maintained in organ culture for 3 days, then assessed by light and electron microscopy. The epithelia from base line uncultured stomachs were stratified and morphologically undifferentiated. During culture in basic medium (Leibovitz L 15), modest maturation of antral and fundic architecture occurred, characterized by epithelial invagination to produce small pit-glands. Secretory granules appeared in occasional epithelial cells, and cytochemistry indicated that most were mucous granules. Addition of pentagastrin (10⁻⁹ to 2 × 10⁻⁷ M) did not induce further morphological maturation in this system. However, addition of cortisol (10⁻⁶ to 10⁻⁵ M) resulted in a marked, dose-related increase of pit-gland formation and of cytological differentiation (appearance of secretory granules). This cortisol-induced architectural maturation was completely inhibited by the mold metabolite cytochalasin B (10⁻⁵ M). The results indicate that fetal gastric maturation can be partially reproduced by culture in chemically defined media, and also suggest that corticosteroids may plan an important role in gastric organogenesis.

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: This work was presented in part at tbe Annual Meeting of tbe American Gastroenterology Association, San Antonio, Teus, May 1975.

: This investigation was supported by Research Grant AM-17537 from the National Institutes of Healt, Bethesda, Maryland

¹: Dr. Yeomans was supported in part by the Edward Wilson Memorial Fellowship, Alfred Hospital, Melbourne, Australia.

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Maturation and Differentiation of Cultured Fetal Stomach: Effects of Corticosteroids, Pentagastrin, and Cytochalasin B

Abstract

Gastroenterology

Gastroenterology

Dev BioI

Gastroenterology

Gastroenterology

A selective effect of gastrin on parietal cell proliferation. Gastroenterology

Gastroenterology

Life Sci

Inhibition of sugar uptake in cultured cells. J Bioi Chem

Effects of steroids on maturation and differentiation of cultured fetal stomach (abstr)

Gastroenterology

The development of the gastro-intestinal tract of the rat

I. Histogenesis of the epithelium of the stomach, small intestine and pancreas. J Morphol

Etude experiment ale de l'organogenese de l'estomac chez Ie foetus de lapin

Developpement normal. Localization experimentale de I'ebauche presomptive. Developpement en cuI. ture “in vitro”" et en greffe “in ovo”. Wilhelm Roux Arch Entwicklungsmech Org

Pentagastrin-induced stimulation of protein synthesis in the gastrointestinal tract

Am J Physiol

Quantitative determination of phosphatase activity in chick embryo duodenum cultured in fluid media with and without hydrocortisone

Nature

Intestinal invertase: precocious development of activity after injection of hydrocortisone

Science

Apparition de canalicules hiliaires dans Ie foie foetel de rat en culture cellulaire BOUS I'influence de glucocorticoides

C R Acad Sci [D] (Paris)

Microfilaments in cellular and developmental processes

Science

Organ cultures of ciliated epithelium for the study of respiratory viruses

Acta Pathol Microbiol Scand

Establishment of human fetal intestinal organ cultures for growth of viruses

J Infect Dis