An improved method for the detection of caeruloplasmin☆

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Abstract

An improved method for the detection of caeruloplasmin is based upon the intensification in the presence of a coupling reagent, 2-cyano-acetylcumarone, of the colour produced by its oxidation of p-phenylenediamine. The colour produced was approximately 6 times denser than that produced in the presence of p-phenylenediamine alone. The method has the advantage that shorter incubation times can be used, thus preserving resolution by minimising diffusion after zone electrophoresis.

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☆

The expenses of this investigation were defrayed in part by a Grant from the National Health and Medical Research Council, Canberra, Australia.

1

Postal address: Commercial Road, Prahran S. I., Victoria, Australia.

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