Isolation of neonatal cardiomyocytes reduces the expression of the GTP-binding protein, Gh

https://doi.org/10.1016/S0022-2828(95)92127-3Get rights and content

α1-Adrenoceptors in most tissues couple with the heterotrimeric GTP-binding protein Gq, the α subunit of which activate the β-isoforms of phospholipase C. However, in heart (and in liver) α1-adrenoceptors have been reported to couple to a high molecular weight GTP-bindingprotein, Gh, which functions both as a type II transglutaminase and as a receptor coupling protein. Gh activates a phospholipase isoform distinct from phospholipase C-β. Here we report that isolation and culture of neonatal cardiomyocytes decreased the expression of Gh without reducing the content of Gq or Gl. Gh was readily detected in extracts from intact neonatal and adult heart tissues. The expression of Gh thus appears to be a feature of intact cardiac tissue.

Cited by (0)

View full text