Hostname: page-component-848d4c4894-wg55d Total loading time: 0 Render date: 2024-05-12T02:49:04.393Z Has data issue: false hasContentIssue false
  • English
  • Français

Methods for the isolation and analysis of plasmodial mutants in Physarum polycephalum

Published online by Cambridge University Press:  14 April 2009

D. J. Cooke  and
Jennifer Dee
D. J. Cooke
Affiliation:
Department of Genetics, University of Leicester, Leicester LEI 7RH, England
Jennifer Dee
Affiliation:
Department of Genetics, University of Leicester, Leicester LEI 7RH, England
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Amoebae of the Colonia isolate of Physarum polycephalum produce plasmodia within individual amoebal clones.

This paper reports the derivation from the Colonia strain C50 of a strain CL (Colonia Leicester) which produces plasmodia in clones with high efficiency and which completes the life cycle reliably and repeatedly in single clones. The derivation of a line CLd (CL delayed plasmodium formation) is described and, using CLd, the construction of the isogenic heterothallic strains mt1; CL and mt2; CL.

The above strains provide a system for the isolation and genetic analysis of mutants of P. polycephalum within a uniform genetic background, in particular mutants expressed in the plasmodium.

Using this system two auxotrophic mutants have been isolated. Preliminary genetic analysis has shown that they are due to single gene mutations.

Type
Research Article
Information
Genetics Research , Volume 24 , Issue 2 , October 1974 , pp. 175 - 187
Copyright
Copyright © Cambridge University Press 1974

References

REFERENCES

Cooke, D. J. & Dee, J. (1974). Plasmodium formation without change in nuclear DNA content in Physarum polycephalum. Genetical Research 23, 307–318.CrossRefGoogle ScholarPubMed
Dee, J. (1966). Multiple alleles and other factors affecting plasmodium formation in the true slime mould Physarum polycephalum Schw. Journal of Protozoology 13, 610–616.CrossRefGoogle Scholar
Dee, J. (1973). Aims and techniques of genetic analysis in Physarum polycephalum. Berichte der Deutschen Botanischen Gesellschaft 86, 93–121.CrossRefGoogle Scholar
Dee, J. & Poulter, R. T. M. (1970). A gene conferring actidione resistance and abnormal morphology on Physarum polycephalum plasmodia. Genetical Research 15, 33–41.CrossRefGoogle Scholar
Dee, J., Wheals, A. E. & Holt, C. E. (1973). Inheritance of plasmodial valine requirement in Physarum polycephalum. Genetical Research 21, 87–101.CrossRefGoogle Scholar
Haugli, F. B. & Dove, W. F. (1972). Mutagenesis and mutant selection in Physarum polycephalum. Molecular and General Genetics 118, 109–124.CrossRefGoogle ScholarPubMed
Haugli, F. B., Dove, W. F. & Jimenez, A. (1972). Genetics and biochemistry of cyclo-heximide resistance in Physarum polycephalum. Molecular and General Genetics 118, 97–107.CrossRefGoogle Scholar
Hutterman, A. (1973). (Ed.) Physarum polycephalum – object of research in cell biology. Berichte der Deutschen Botanischen Gesellschaft 86, 1–179.CrossRefGoogle Scholar
Mohberg, J., Babcock, K. L., Haugli, F. B. & Rusch, H. P. (1973). Nuclear DNA content and chromosome numbers in the Myxomycete Physarum polycephalum. Developmental Biology 34, 228–245.CrossRefGoogle ScholarPubMed
Poulter, R. T. M. (1969). Senescence in the myxomycete Physarum polycephalum Ph.D. Thesis, University of Leicester.Google Scholar
Poulter, R. T. M. & Dee, J. (1968). Segregation of factors controlling fusion between plasmodia of the true slime mould Physarum polycephalum. Genetical Research 12, 71–79.CrossRefGoogle ScholarPubMed
Wheals, A. E. (1970). A homothallic strain of the Myxomycete Physarum polycephalum. Genetics 66, 623–633.CrossRefGoogle ScholarPubMed
Wheals, A. E. (1973). Developmental mutants in a homothallic strain of Physarum polycephalum. Genetical Research 21, 79–86.CrossRefGoogle Scholar