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Variations in the levels of acute-phase proteins and lactoferrin in serum and milk during bovine subclinical mastitis

Published online by Cambridge University Press:  12 August 2021

Aarsha Raj ,
Vinodkumar Kulangara ,
Tresamol P. Vareed ,
Deepa P. Melepat ,
Latha Chattothayil  and
Sunanda Chullipparambil
Aarsha Raj
Affiliation:
Multi-Speciality Veterinary Hospital, Thiruvanathapuram, Kerala, India
Vinodkumar Kulangara*
Affiliation:
Department of Veterinary Epidemiology and Preventive Medicine, Kerala, India
Tresamol P. Vareed
Affiliation:
Department of Veterinary Epidemiology and Preventive Medicine, Kerala, India
Deepa P. Melepat
Affiliation:
Department of Veterinary Epidemiology and Preventive Medicine, Kerala, India
Latha Chattothayil
Affiliation:
Department of Veterinary Public Health, Kerala, India
Sunanda Chullipparambil
Affiliation:
Department of Statistics, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala, India
*
Author for correspondence: Vinodkumar Kulangara, Email: kvinodkuma@gmail.com
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Abstract

Variations in the levels of acute phase proteins and lactoferrin in serum and milk for diagnosis of subclinical mastitis in dairy cows are described in this research paper. Milking animals from two organized dairy farms in Kerala, India, were screened by California Mastitis Test (CMT), Electrical Conductivity test (EC) and Somatic Cell Count (SCC) test to identify animals affected with sub clinical mastitis (SCM). The concentrations of acute phase proteins (APP) Haptoglobin (Hp), C- reactive protein (CRP), Albumin, Lactoferrin (Lf) and α- 1 acid glycoprotein (AGP) in milk and Hp, Albumin, Serum Amyloid A (SAA) and CRP in the serum of 40 normal cows and 40 cows affected with sub clinical mastitis were assessed. Solid phase ELISA was employed for assessment of all parameters except the albumin levels, for which spectrophotometry was used. The values of Hp in milk; and SAA, AGP and Lf in serum, were significantly elevated in the group with sub clinical mastitis. Such variations were found to be independent of the specific bacterial organism causing the disease. These results show that significant variations exist in the levels of acute phase proteins Hp, AGP and Lf in milk, and SAA in serum of animals affected with subclinical bovine mastitis that are not affected by specific bacterial etiology.

Keywords

Bovinesubclinical mastitisacute phase proteins
Type
Research Article
Information
Journal of Dairy Research , Volume 88 , Issue 3 , August 2021 , pp. 321 - 325
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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