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Molecular evidence for the presence of a G7 genotype of Echinococcus granulosus in Slovakia

Published online by Cambridge University Press:  12 April 2024

V. Šnábel ,
S. D'Amelio ,
K. Mathiopoulos ,
L. Turčeková  and
P. Dubinský
V. Šnábel*
Affiliation:
Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 04001 Košice, Slovakia:
S. D'Amelio
Affiliation:
Institute of Parasitology, University of Rome 'La Sapienza', Piazzale Aldo Moro 5, 00185 Rome, Italy
K. Mathiopoulos
Affiliation:
Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 04001 Košice, Slovakia:
L. Turčeková
Affiliation:
Institute of Parasitology, University of Rome 'La Sapienza', Piazzale Aldo Moro 5, 00185 Rome, Italy
P. Dubinský
Affiliation:
Institute of Parasitology, University of Rome 'La Sapienza', Piazzale Aldo Moro 5, 00185 Rome, Italy
*
*Fax: 421 95 63 31414 E-mail: snabel@saske.sk
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Abstract

Variability in Echinococcus granulosus is very important epidemiologically since strain characteristics may influence local patterns of transmission of hydatid disease. To classify the genotype presented in pig protoscoleces of the Slovak territory, a DNA-based approach has been used. Nucleotide sequences for a 471 bp region of the mitochondrial NADH dehydrogenase 1 (ND1) gene revealed a substantial affinity of isolates examined to the G7 genotype. Only a 0.9–3.4% sequence variation was recorded for E. granulosus samples compared with the reference G7 variant. To distinguish between G7 and G9 genotypes not differing in ND1 sequences, isolates were additionally examined by PCR-RFLP analysis of the nuclear ITS1 region. The resulting two-banded pattern is characteristic for the G7 strain. The data presented thus provides the first explicit evidence of the G7 genotype in the Slovak region.

Type
Research Article
Information
Journal of Helminthology , Volume 74 , Issue 2 , June 2000 , pp. 177 - 181
Copyright
Copyright © Cambridge University Press 2000

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