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The source, release and specificity of proteolytic enzyme activity produced by Anisakis simplex larvae (Nematoda: Ascaridida) in vitro

Published online by Cambridge University Press:  05 June 2009

Bernard E. Matthews
Bernard E. Matthews
Affiliation:
Department of Zoology, University of Edinburgh, West Mains Road, Edinburgh E119 3JT, U.K.
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Abstract

Indirect fluorescent antibody techniques and incubation of sectioned and ligatured larvae have been used to locate the source of azocoll-positivc secretions within the oesophagcal glands of third-stage Anisakis simplex larvae. The “excretory” system, previously suggested as a source of invasive enzymes, docs not seem to be involved. The proteolytic enzyme has trypsin-like properties and evidence is presented for its continuous synthesis during in vitro storage.

Type
Research Article
Information
Journal of Helminthology , Volume 58 , Issue 3 , September 1984 , pp. 175 - 185
Copyright
Copyright © Cambridge University Press 1984

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References

REFERENCE

Berland, B. (1961) Nematodes from some Norwegian marine fishes. Sarsia, 2, 1–50.Google Scholar
Beverley-burton, M., Nyman, O.L. & Pippy, J.H.C. (1977) The morphology, and some observations on the population genetics of Anisakis simplex larvae (Nematoda: Ascaridata) from fishes of the North Atlantic. Journal of the Fisheries Research Board of Canada,. 34, 105–112.CrossRefGoogle Scholar
Bird, A.F. (1967) Changes associated with parasitism in nematodes. I. Morphology and physiology of preparasitic and parasitic larvae of Meloidogyne javanica. Journal of Parasitology, 53, 768–776.CrossRefGoogle ScholarPubMed
Bird, A.F. (1971) The Structures of Nematodes. Academic Press. New York & London.Google Scholar
Charney, J. & Tomarelli, R.M. (1947) A colorimctric method for the determination of the proteolytic activity of duodenal juice. Journal of Biological Chemistry, 171, 501–505.CrossRefGoogle ScholarPubMed
Chitwood, B.G. (1938) Notes on the physiology of Ascaris lumbricoides. Proceedings of the Helminthological Society of Washington, 5, 18–19.Google Scholar
Croll, N.A., Ma, K., Smith, J.M., Sukhdeo, M.V. & Wild, C. (1980) Phocanema decipiens: intestinal penetration in the laboratory rat. Experimental Parasitology, 50, 145–154.CrossRefGoogle ScholarPubMed
Davey, R.G. & Sommerville, R.I. (1974) Moulting in a parasitic nematode, Phocanema decipiens. VII. The mode of action of the ecdysial hormone. International Journal for Parasitology, 4, 241–259.CrossRefGoogle Scholar
Fairbairn, D. (1960) The physiology and biochemistry of nematodes. In “Nematology”, (J. N. Sasser & W. R. Jenkins, Editors). University of North Carolina Press.Google Scholar
Gross, J. & Kirk, D. (1958) The heat precipitation of collagen from neutral salt solutions: some rate regulating factors. Journal of Biological Chemistry, 233, 355–360.CrossRefGoogle ScholarPubMed
Hoeppli, R. (1933) On histolytic changes and cxtra-intcstinal digestion in parasitic infection. Lingnam Science Journal, 12, Suppl. 1–11.Google Scholar
Hsu, H.F. (1933) Study on the oesophegcal glands of parasitic nematoda superfamily Ascaroidca. Chinese Medical Journal, 47, 1247–1288.Google Scholar
Jarumilinta, R. & Maegraith, B.G. (1961)The patterns of some proteolytic enzymes of Entamoeba histolytica and Acanthamoeba sp. II. The action of E. histolytica and Acanthamoeba sp. on various synthetic substrates. Annals of Tropical Medicine and Parasitology, 55, 518–528.CrossRefGoogle ScholarPubMed
Lee, H.F., Chen, I-LI & Lin, R.P. (1973) Ultrastructure of the excretory system of Anisakis larva (Nematoda: Anisakidae). Journal of Parasitology, 59, 289–298.CrossRefGoogle ScholarPubMed
Matthews, B.E. (1982) Behaviour and enzyme release by Anisakis sp. larvae (Nematoda: Ascaridida). Journal of Helminthology, 56, 177–183.CrossRefGoogle ScholarPubMed
Naughton, M.A. & Sanger, F. (1961) Purification and specificity of pancreatic elastase. Biochemical Journal, 78, 156–163.CrossRefGoogle ScholarPubMed
Ornstein, L. & Davis, B.J. (1964) Disc electrophoresis; background and theory. Annals of the New York Academy of Science, 121, 321–349.CrossRefGoogle ScholarPubMed
Penn, A., Gledhill, B.L. & Dorzynkiewicz, Z. (1972) Modification of the gelatin substrate procedure for demonstration of acrosomal proteolytic activity. Journal of Histochemistry and Cytochemistry, 20, 499–506.CrossRefGoogle ScholarPubMed
Pippy, J.H.C. & VAn banning, P. (1975) Identification of Anisakis larvae (1) as Anisakis simplex (Rudolphi, 1809 det. Krabbe 1878) (Nematoda: Ascaridata). Journal of the Fisheries Research Board of Canada, 32, 29–32.CrossRefGoogle Scholar
Riley, J. (1972) The pathology of Anisakis nematode infections of the fulmar Fulmarus glacialis. Ibis, 114, 102–104.CrossRefGoogle Scholar
Ruitenberg, E.J. & Loendersloot, H.J. (1971) Histochemical properties of the excretory organ of Anisakis sp. larva. Journal of Parasitology, 57, 1149–1150.CrossRefGoogle Scholar
Smith, J.M. (1976) Comparative ultrastructure of the oesophageal glands of third stage larval hookworms. International Journal for Parasitology, 6, 9–13.CrossRefGoogle ScholarPubMed
Smith, J.W. & Wootten, R. (1978) Anisakis and anisakiasis. Advances in Parasitology, 16, 93–163.CrossRefGoogle ScholarPubMed
Sommerville, R.I. & Davey, K.G. (1976) Stimuli for cuticle formation on ecdysis in vitro of the infective larva of Anisakis sp. (Nematoda: Ascaridoidea). International Journal for Parasitology, 6, 433–439.CrossRefGoogle ScholarPubMed
Thorson, R.E. (1956) Proteolytic activity in extracts of the esophagi of adults of Ancylostoma caninum and the effect of immune serum on this activity. Journal of Parasitology, 42, 21–25.CrossRefGoogle Scholar
Vik, R. (1964) Penetration of stomach wall by Anisakis-type larvae in porpoises. Canadian Journal of Zoology, 42, 513.CrossRefGoogle Scholar
Von brand, T. (1973) Biochemistry of Parasites. Academic Press, New York & London.Google Scholar
Wright, D.J. & Newell, D.R. (1976) Nitrogen excretion, osmotic and ionic regulation in nematodes. In: “The Organization of Nematodes”. (N. A. Croll, Editor). Academic Press, London, New York, San Francisco.Google Scholar
Yemm, E.W. & Cocking, E.C. (1955) The determination of amino acid with ninhydrin. The Analyst, 80, 209–213.CrossRefGoogle Scholar
Young, P.C. & Lowe, D. (1969) Larval nematodes from fish of the subfamily Anisakinae and gastrointestinal lesions in mammals. Journal of Comparative Pathology, 79, 301–313.CrossRefGoogle ScholarPubMed