Hostname: page-component-76fb5796d-r6qrq Total loading time: 0 Render date: 2024-04-28T04:04:07.214Z Has data issue: false hasContentIssue false
  • English
  • Français

Increased exposure of parasite antigens at the surface of adult male Schistosoma mansoni exposed to praziquantel in vitro

Published online by Cambridge University Press:  06 April 2009

W. Harnett  and
J. R. Kusel
W. Harnett
Affiliation:
Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ
J. R. Kusel
Affiliation:
Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ
Get access Rights & Permissions [Opens in a new window]

Summary

Praziquantel is a broad-spectrum anthelmintic active against schistosome species which are major parasites of man. Two major effects on Schistosoma mansoni have been demonstrated; (i) spastic paralysis of the parasite musculature, possibly arising as a consequence of an influx of Ca2+ into the worm (Pax, Bennett & Fetterer, 1978; Coles, 1979) and (ii) vacuolation and degeneration of the worm tegument (Becker, Mehlhorn, Andrews, Thomas & Eckert, 1980). These events may contribute to the elimination of schistosomes in vivo, but this elimination may partly be dependent on the host immune response as infected T-cell-deprived mice are less able than immunologically intact animals to reduce their worm burdens following drug treatment (Doenhoff, Harrison, Sabah, Murare, Dunne & Hassounah, 1982). This latter observation raises the possibility that praziquantel may lower the ability of the parasite to evade host immunity by increasing the exposure of parasite antigens capable of acting as targets for host antibody, or antibody-armed cells at the worm surface. Consistent with this idea is the observation that adult schistosomes in praziquantel-treated mice are invaded a few hours after treatment by host granulocytes (Mehlhorn, Becker, Andrews, Thomas & Frenkel, 1981).

Type
Research Article
Information
Parasitology , Volume 93 , Issue 2 , October 1986 , pp. 401 - 405
Copyright
Copyright © Cambridge University Press 1986

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Becker, B., Mehlhorn, H., Andrews, P., Thomas, H. & Eckert, J. (1980). Light and electron microscopic studies on the effect of praziquantel on Schistosoma mansoni, Dicrocoelium dendriticum and Fasciola hepatica (Trematoda) in vitro. Zeitschrift für Parasitenkunde 63, 113–28.CrossRefGoogle ScholarPubMed
Coles, G. C. (1979). The effect of praziquantel on Schistosoma mansoni. Journal of Helminthology 53, 31–3.CrossRefGoogle ScholarPubMed
Doenhoff, M., Harrison, R., Sabah, A., Murare, H., Dunne, D. & Hasseunah, O. (1982). Schistosomiasis in the immunosuppressed host: studies on the host–parasite relationship of Schistosoma mansoni and S. bovis in T-cell-deprived and hydrocortisone-treated mice. In Animal Models in Parasitology (ed. Owen, D. G.), pp. 155–169. London and Basingstoke: Macmillan.CrossRefGoogle Scholar
Goldring, O. L., Sher, A., Smithers, S. R. & McLaren, D. J. (1977). Host antigens and parasite antigens of murine Schistosoma mansoni. Transactions of the Royal Society of Tropical Medicine and Hygiene 71, 144–8.CrossRefGoogle ScholarPubMed
Harnett, W. & Kusel, J. R. (1986). Schistosoma mansoni: in vitro exposure of adult male surface antigens. Experimental Parasitology 61, 146–50.CrossRefGoogle ScholarPubMed
Harnett, W., Kusel, J. R. & Barrowman, M. M. (1985). The use of aldehydes to show a relationship between host and parasite antigens at the surface of adult male Schistosoma mansoni. Parasite Immunology 7, 415–28.CrossRefGoogle ScholarPubMed
Hunter, W. M. & Greenwood, F. C. (1962). Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature, London 194, 495–6.CrossRefGoogle ScholarPubMed
Kusel, J. R., Stones, L. S. & Harnett, W. (1981). The use of membrane-active compounds to examine the structure of the surface membrane of Schistosoma mansoni. Bioscience Reports 1, 253–61.CrossRefGoogle ScholarPubMed
Kusel, J. R., Stones, L. & Harnett, W. (1982). The effects of retinol (vitamin A alcohol) and various non-ionic detergents on the surfaces of schistosomula and adult Schistosoma mansoni. Molecular and Biochemical Parasitology 5, 147–63.CrossRefGoogle ScholarPubMed
McLaren, D. J., Hockley, D. J., Goldring, O. L. & Hammond, B. J. (1978). A freeze fracture study of the developing tegumental outer membrane of Schistosoma mansoni. Parasitology 76, 327–48.CrossRefGoogle ScholarPubMed
Mehlhorn, H., Becker, B., Andrews, P., Thomas, H. & Frenkel, J. K. (1981). In vivo and in vitro experiments on the effects of praziquantel on Schistosoma mansoni. Arzneimittelforsch 31, 544–54.Google ScholarPubMed
Mehlhorn, H., Frenkel, J. K., Andrews, P. & Thomas, H. (1982). Light and electron microscope studies on Schistosoma mansoni granulomas of mouse livers following treatment with praziquantel. Tropenmedizin und Parasitologie 33, 229–39.Google ScholarPubMed
Pax, R., Bennett, J. L. & Fetterer, R. (1978). A benzodiazepine derivative and praziquantel: effects on musculature of Schistosoma mansoni and Schistosoma japonicum. Naunyn-Schmiedeberg's Archives of Pharmacology 304, 309–15.CrossRefGoogle ScholarPubMed
Smithers, S. R. & Terry, R. J. (1965). The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of the adult worms. Parasitology 55, 695–701.CrossRefGoogle ScholarPubMed