Hostname: page-component-848d4c4894-x24gv Total loading time: 0 Render date: 2024-05-05T22:24:30.960Z Has data issue: false hasContentIssue false
  • English
  • Français

Isolation of Theileria parasites from African buffalo (Syncerus caffer) and characterization with anti-schizont monoclonal antibodies

Published online by Cambridge University Press:  06 April 2009

P. A. Conrad ,
D. A. Stagg ,
J. G. Grootenhuis ,
A. D. Irvin ,
J. Newson ,
R. E. G. Njamunggeh ,
P. B. Rossiter  and
A. S. Young
P. A. Conrad
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
D. A. Stagg
Affiliation:
Veterinary Research Department, Kenya Agricultural Research Institute (KARI), P.O. Box 32, Kikuyu, Kenya
J. G. Grootenhuis
Affiliation:
Wildlife Diseases Research Project, Veterinary Research Laboratory, P.O. Kabete, Kenya
A. D. Irvin
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
J. Newson
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
R. E. G. Njamunggeh
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
P. B. Rossiter
Affiliation:
Veterinary Research Department, Kenya Agricultural Research Institute (KARI), P.O. Box 32, Kikuyu, Kenya
A. S. Young
Affiliation:
Veterinary Research Department, Kenya Agricultural Research Institute (KARI), P.O. Box 32, Kikuyu, Kenya
Get access Rights & Permissions [Opens in a new window]

Extract

Antigenic differences between intra-lymphocytic theilerial parasites isolated from the blood of 18 African buffalo and grown in vitro were assessed with anti-schizont monoclonal antibodies (mAbs). There was marked antigenic diversity both between isolates from different buffalo and between isolates taken at different times from the same buffalo. Many of the isolates from both wild and captive buffalo appeared to consist of mixed parasite populations. Some isolates were found by limiting dilution cloning and mAb testing to contain at least 3 or 4 distinct populations of Theileria. Once cloned, Theileria-infected lymphoblastoid cell lines retained their mAb profiles during prolonged in vitro cultivation and, when recloned, the subclones had the same mAb profile as their parent clone. The implications of these results for further studies on buffalo-derived theilerial parasites are discussed.

Type
Research Article
Information
Parasitology , Volume 94 , Issue 3 , June 1987 , pp. 413 - 423
Copyright
Copyright © Cambridge University Press 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Barnett, S. F. & Brocklesby, D. W. (1959). Theileria lawrencei in Kenya. Bulletin of Epizootic Diseases of Africa 7, 345–7.Google Scholar
Barnett, S. F. & Brocklesby, D. W. (1966 a). The passage of ‘Theileria lawrencei (Kenya)’ through cattle. British Veterinary Journal 122, 396–409.Google Scholar
Barnett, S. F. & Brocklesby, D. W. (1966 b). The susceptibility of African buffalo (Syncerus caffer) to infection with Theileria parva (Theiler, 1904). British Veterinary Journal 122, 379–86.CrossRefGoogle Scholar
Brocklesby, D. W. & Barnett, S. F. (1966). The literature concerning Theileridae of the African buffalo (Syncerus caffer). British Veterinary Journal 122, 371–9.CrossRefGoogle Scholar
Brocklesby, D. W., Barnett, S. F. & Scott, G. R. (1961). Morbidity and mortality rates in East Coast fever (Theileria parva infection) and their application to drug screening procedures. British Veterinary Journal 117, 529–31.CrossRefGoogle Scholar
Brown, C. G. D. (1983). Theileria. In In Vitro Cultivation of Protozoan Parasites (ed. Jensen, J. B.), pp. 243–84. Boca Raton, Florida: CRC Press, Inc.Google Scholar
Dolan, T. T., Radley, D. E., Brown, C. G. D., Cunningham, M. P., Morzaria, S. P. & Young, A. S. (1980). East Coast fever: 4. Further studies on the protection of cattle immunized with a combination of theilerial strains. Veterinary Parasitology 6, 325–32.Google Scholar
Grootenhuis, J. G., Karstad, L. & Drevemo, S. A. (1976). Experience with drugs for capture and restraint of wildebeest, impala, eland and hartebeest in Kenya. Journal of Wildlife Diseases 12, 435–43.CrossRefGoogle ScholarPubMed
Grootenhuis, J. G., Leitch, B. L., Stagg, D. A., Dolan, T. T. & Young, A. S. (1987). Experimental induction of Theileria parva lawrencei carrier state in an African buffalo (Syncerus caffer). Parasitology 94, 425–31.Google Scholar
Irvin, A. D., Dobbelaere, D. A. E., Mwamachi, D. M., Minami, T., Spooner, P. R. & Ocama, J. G. R. (1983). Immunisation against East Coast fever: correlation between monoclonal antibody profiles of Theileria parva stocks and cross immunity in vivo. Research in Veterinary Science 35, 341–6.CrossRefGoogle ScholarPubMed
Irvin, A. D., Purnell, R. E., Brown, C. G. D., Cunningham, M. P., Ledger, M. A. & Payne, R. C. (1974). The application of an indirect method of infecting ticks with piroplasms for use in the isolation of field infections. British Veterinary Journal 130, 280–7.CrossRefGoogle ScholarPubMed
Kearney, J. F., Radbruch, A., Liesegang, B. & Rajewsky, K. (1979). A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. Journal of Immunology 123, 1548–50.CrossRefGoogle ScholarPubMed
Köhler, G., Howe, S. C. & Milstein, C. (1976). Fusion between immunoglobutin-secreting and nonsecreting myeloma cell lines. European Journal of Immunology 6, 292–5.Google Scholar
Kurtti, T. J., Munderloh, U. G., Irvin, A. D. & Büscher, G. (1981). Theileria parva: early events in the development of bovine lymphoblastoid cell lines persistently infected with macroschizonts. Experimental Parasitology 52, 280–90.CrossRefGoogle ScholarPubMed
Lohr, K. F. (1978). A hypothesis on the role of Theileria lawrencei in the epizootiology of East Coast fever and on its importance in attempting vaccination. In Tick-borne Diseases and their Vectors, (ed. Wilde, J. H. K.), pp. 315–17. Edinburgh: Centre for Tropical Veterinary Medicine.Google Scholar
Minami, T., Spooner, P. R., Irvin, A. D., Ocama, J. G. R., Dobbelaere, D. A. E. & Fujinaga, T. (1983). Characterisation of stocks of Theileria parva by monoclonal antibody profiles. Research in Veterinary Science 35, 334–40.CrossRefGoogle ScholarPubMed
Neitz, W. O. (1955). Corridor Disease: a fatal form of bovine Theileriosis encountered in Zululand. Bulletin of Epizootic Diseases of Africa 3, 121–3.Google Scholar
Neitz, W. O. (1957). Theileriosis, Gonderioses and Cytanxzoonoses: a review. Onderstepoort Journal of Veterinary Research 27, 275–430.Google Scholar
Neitz, W. O., Canham, A. S. & Kluge, E. B. (1955). Corridor Disease: a fatal form of bovine Theileriosis encountered in Zululand. Journal of the South African Veterinary Medical Association 26, 79–87.Google Scholar
Newson, J., Naessens, J., Stagg, D. A. & Black, S. J. (1986). A cell surface antigen associated with Theileria parva lawrencei-infected bovine lymphoid cells. Parasite Immunology 8, 149–58.CrossRefGoogle ScholarPubMed
Pearson, T. W., Pinder, M., Roelants, G. E., Kar, S. K., Lundin, L. B., Mayor-Withey, K. S. & Hewett, R. S. (1960). Methods for derivation and detection of anti-parasite monoclonal antibodies. Journal of Immunological Methods 34, 141–54.CrossRefGoogle Scholar
Pinder, M. & Hewett, R. S. (1980). Monoclonal antibodies detect antigenic diversity in Theileria parva parasites. Journal of Immunology 124, 1000–1.CrossRefGoogle ScholarPubMed
Radley, D. E., Brown, C. G. D., Burridge, M. J., Cunningham, M. P., Kirimi, I. M., Purnell, R. E. & Young, A. S. (1975). East Coast fever: 1. Chemoprophylactic immunization of cattle against Theileria parva (Muguga) and five theilerial strains. Veterinary Parasitology 1, 35–41.CrossRefGoogle Scholar
Radley, D. E., Young, A. S., Brown, C. G. D., Burridge, M. J., Cunningham, M. P., Musisi, F. L. & Purnell, R. E. (1975). East Coast fever: 2. Cross-immunity trials with a Kenya strain of Theileria lawrencei. Veterinary Parasitology 1, 43–50.CrossRefGoogle Scholar
Radley, D. E., Young, A. S., Grootenhuis, J. G., Cunningham, M. P., Dolan, T. T. & Morzaria, S. P. (1979). Further studies on the immunization of cattle against Theileria lawrencei by infection and chemoprophylaxis. Veterinary Parasitology 5, 117–28.CrossRefGoogle Scholar
Shiels, B., McDougall, C., Tait, A. & Brown, C. G. D. (1986). Antigenic diversity of Theileria annulata macroschizonts. Veterinary Parasitology 21, 1–10.CrossRefGoogle ScholarPubMed
Stagg, D. A., Brown, C. G. D., Crawford, J. G., Kanhai, G. K. & Young, A. S. (1974). In vitro cultivation of Theileria lawrencei-infected lymphoblastoid cell lines derived from a buffalo (Syncerus caffer). Research in Veterinary Science 16, 125–7.CrossRefGoogle ScholarPubMed
Stagg, D. A., Young, A. S., Leitch, B. L., Grootenhuis, J. G. & Dolan, T. T. (1983). Infection of mammalian cells with Theileria species. Parasitology 86, 243–54.Google Scholar
Uilenberg, G. (1981). Theilerial species of domestic livestock. In Advances in the Control of Theileriosis, (ed. Irvin, A. D., Cunningham, M. P. and Young, A. S.), pp. 4–37. London: Martinus Nijhoff.CrossRefGoogle Scholar
Uilenberg, G., Perié, N. M., Lawrence, J. A., de Vos, A. J., Paling, R. W. & Spanjer, A. A. M. (1982). Causal agents of bovine theileriosis in Southern Africa. Tropical Animal Health and Production 14, 127–40.CrossRefGoogle ScholarPubMed
Young, A. S., Branagan, D., Brown, C. G. D., Burridge, M. J., Cunningham, M. P. & Purnell, R. E. (1973). Preliminary observations on a theilerial species pathogenic to cattle isolated from buffalo (Syncerus caffer) in Tanzania. British Veterinary Journal 129, 382–9.Google Scholar
Young, A. S., Brown, C. G. D., Burridge, M. J., Cunningham, M. P., Kirimi, I. M. & Irvin, A. D. (1973). Observations on the cross-immunity between Theileria lawrencei (Serengeti) and Theileria parva (Muguga) in cattle. International Journal for Parasitology 3, 723–8.Google Scholar
Young, A. S., Brown, C. G. D., Burridge, M. J., Cunningham, M. P., Payne, R. C. & Purnell, R. E. (1977 a). Establishment of an experimental field population of Theileria lawrencei-infected ticks maintained by African buffalo (Syncerus caffer). Journal of Parasitology 63, 903–7.Google Scholar
Young, A. S., Brown, C. G. D., Burridge, M. J., Grootenhuis, J. G., Kanhai, G. K., Purnell, R. E. & Stagg, D. A. (1978 a). The incidence of theilerial parasites in East African buffalo (Syncerus caffer). Tropenmedizin und Parasitologie 29, 281–9.Google Scholar
Young, A. S., Brown, C. G. D., Cunningham, M. P. & Radley, D. E. (1978 b). Evaluation of methods of immunizing cattle against Theileria lawrencei. In Tick-borne Diseases and Their Vectors (ed. Wilde, J. H. K.), pp. 293–6. Edinburgh: Centre for Tropical Veterinary Medicine.Google Scholar
Young, A. S. & Purnell, R. E. (1973). Transmission of Theileria lawrencei (Serengeti) by the ixodid tick, Rhipicephalus appendiculatus. Tropical Animal Health and Production 5, 146–52.Google Scholar
Young, A. S., Radley, D. E., Cunningham, M. P., Musisi, F. L., Payne, R. C. & Purnell, R. E. (1977 b). Exposure of immunized cattle to prolonged natural challenge of Theileria lawrencei derived from African buffalo (Syncerus caffer). Veterinary Parasitology 3, 283–90.Google Scholar