Hostname: page-component-76fb5796d-vfjqv Total loading time: 0 Render date: 2024-04-27T22:49:49.323Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of immunity upon some enzymes of the parasitic nematode, Nippostrongylus brasiliensis

Published online by Cambridge University Press:  06 April 2009

A. J. Edwards ,
J. S. Burt  and
Bridget M. Ogilvie
A. J. Edwards
Affiliation:
National Institute for Medical Research, Mill Hill, London, N.W.7
J. S. Burt
Affiliation:
National Institute for Medical Research, Mill Hill, London, N.W.7
Bridget M. Ogilvie
Affiliation:
National Institute for Medical Research, Mill Hill, London, N.W.7
Get access Rights & Permissions [Opens in a new window]

Extract

Isoenzyme patterns of normal adult Nippostrongylus brasiliensis were compared with those of damaged worms permanently affected by immunity and adapted worms which show increased resistance to immunity.

Isoenzymes of acetylcholinesterase, found in glands of the head end of the worms, showed changes in damaged and adapted worms which can be related to the effects of immunity.

Aminopeptidases and non-specific esterases from the same glands were unchanged in worms affected by immunity.

Isoenzymes of acid phosphatase found in the gut of the worms were altered in damaged and adapted worms; these changes are probably secondary to the changes in acetylcholinesterases.

Of the enzymes studied, acetylcholinesterase is the enzyme most likely to be the antigen which stimulates host immunity, but final proof of this is required.

We are most grateful to Dr Valerie E. Jones for many helpful suggestions.

Type
Research Article
Information
Parasitology , Volume 62 , Issue 2 , April 1971 , pp. 339 - 347
Copyright
Copyright © Cambridge University Press 1971

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Akroyd, P. (1968). Vertical flat bed gel electrophoresis. In Chromatographic and Electrophoretic Techniques, vol. 2, 2nd edition. Ed. Smith, I.. London: Heinemann.Google Scholar
Burt, J. S. (1970). A histochemical study of esterases in the nematode, Nippostrongylus brasiliensis. M.I.Biol. Thesis, Institute of Biology, London.Google Scholar
Chandler, A. C. (1932). Susceptibility and resistance to helminthic infections. Journal of Parasitology 18, 135–52.CrossRefGoogle Scholar
Gomori, G. (1950). An improved chemical technique for acid phosphatase. Stain Technology 25, 81–5.CrossRefGoogle Scholar
Grunder, A. A., Sartore, G. & Stormont, C. (1965). Genetic variation in red cell esterases of rabbits. Genetics 52, 1345–53.CrossRefGoogle ScholarPubMed
Holt, S. J. (1954). A new approach to the cytochemical localisation of enzymes. Proceedings of the Royal Society, Series B, 142, 160–9.Google Scholar
Jackson, G. J. (1960). Fluorescent antibody studies of Nippostrongylus muris infections. Journal of Infectious Diseases 106, 20–36.CrossRefGoogle ScholarPubMed
Jones, V. E. & Ogilvie, B. M. (1971). Protective immunity to Nippostrongylus brasiliensis: the sequence of events which expels worms from the rat intestine. Immunology (in Press).Google ScholarPubMed
Lee, D. L. (1969). Changes in adult Nippostrongylus brasiliensis during the development of immunity to this nematode in rats. I. Changes in ultrastructure. Parasitology 59, 29–39.CrossRefGoogle Scholar
Lee, D. L. (1970). The fine structure of the excretory system in adult Nippostrongylus brasiliensis (Nematoda) and a suggested function for the ‘excretory glands’. Tissue & Cell 2, 225–231.CrossRefGoogle Scholar
Ogilvie, B. M. (1969). Immunity to Nippostrongylus brasiliensis. Symposia of the British Society for Parasitology 7, 31–40.Google Scholar
Ogilvie, B. M. & Hockley, D. J. (1968). Effects of immunity on Nippostrongylus brasiliensis adult worms: reversible and irreversible changes in infectivity, reproduction, and morphology. Journal of Parasitology 54, 1073–84.CrossRefGoogle ScholarPubMed
Ogilvie, B. M. & Jones, V. E. (1971). Nippostrongylus brasiliensis. A review of immunity and the host/parasite relationship in the rat. Experimental Parasitology (in Press).CrossRefGoogle ScholarPubMed
Pearse, A. G. E. (1960). Histochemistry, Theoretical and Applied. London: Churchill.Google Scholar
Rhodes, M. B., Marsh, C. L. & Kelly, G. W. (1964). Studies in helminth enzymology. III. Malic dehydrogenase in Ascaris suum. Experimental Parasitology 15, 403–9.CrossRefGoogle Scholar
Sanderson, B. E. (1969). Acetylcholinesterase activity in Nippostrongylus brasiliensis (Nematoda). Comparative Biochemistry and Physiology 29, 1207–13.CrossRefGoogle ScholarPubMed
Sarles, M. P. (1938). The in vitro action of immune rat serum on the nematode, Nippostrongylus muris. Journal of Infectious Diseases 62, 337–48.CrossRefGoogle Scholar
Shaw, C. R. & Koen, A. L. (1968). Starch gel zone electrophoresis of enzymes. In Chromatographic and Electrophoretic Techniques, vol. 2., 2nd edition. Ed. Smith, I.. London: Heinemann.Google Scholar
Weinstein, P. P. (1966). The in vitro cultivation of helminths with reference to morphogenesis. In Biology of Parasites, Ed. Soulsby, E. J. L.. New York, London: Academic Press.Google Scholar