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Onset of water stress tolerance in developing Helianthus annuus embryos

Published online by Cambridge University Press:  19 September 2008

M. Th. Le Page-Degivry  and
G. Garello
M. Th. Le Page-Degivry*
Affiliation:
Laboratoire de Physiologie Végétale, Université de Nice, Sophia Antipolis 06034 NICE Cedex, France
G. Garello
Affiliation:
Laboratoire de Physiologie Végétale, Université de Nice, Sophia Antipolis 06034 NICE Cedex, France
*
* Correspondence
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Abstract

Excised Helianthus annuus embryos were capable of precocious germination as early as 7 d after pollination. At this stage they were not capable of withstanding desiccation treatment. The desiccation tolerance was acquired at a stage of development which depended on the rate of water loss to which embryos were subjected. Coincident with the acquisition of tolerance to a rapid desiccation, they gained the capacity to withstand 24 h preincubation in agitated water; this acquisition could be correlated to a sharp decrease of the leaching of electrolytes, amino acids and proteins in the incubation water, suggesting that a change in membrane properties played a key role in this transition. Moreover, the acquisition of tolerance to these stresses was coincident with the increase of endogenous abscisic acid (ABA). ABA applied in vitro to young embryos induced tolerance both to desiccation and to preincubation in water: this in vitro acquisition of tolerance was again correlated with a decrease in the leakage.

Keywords

abscisic aciddesiccation tolerancedeveloping embryosHelianthus annuusmembrane leakagesunflower
Type
Research Papers
Information
Seed Science Research , Volume 1 , Issue 4 , December 1991 , pp. 221 - 227
Copyright
Copyright © Cambridge University Press 1991

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References

Adams, C.A., Fjerstad, M.C. and Rinne, R.W. (1983) Characteristics of soybean seed maturation: necessity for slow dehydration. Crop Science 23, 265–267.CrossRefGoogle Scholar
Bartels, D., Singh, M. and Salamini, F. (1988) Onset of desiccation tolerance during development of the barley embryos. Planta 175, 485–492.CrossRefGoogle Scholar
Becwar, M.R., Stanwood, P.C. and Roos, E.E. (1982) Dehydration effects on imbibitional leakage from desiccation sensitive seeds. Plant Physiology 69, 1132–1135.CrossRefGoogle ScholarPubMed
Bedford, L.V. and Matthews, S. (1976) The effect of seed age at harvest on the germinability and quality of heatdried seed peas. Seed Science and Technology 4, 275–286.Google Scholar
Bochiccio, A., Vazzana, C., Raschi, A., Bartels, D. and Salamini, F. (1988) Effect of desiccation on isolated embryos of maize. Onset of desiccation tolerance during development. Agronomie 8, 29–36.CrossRefGoogle Scholar
Chin, H.F., Krishnapillay, B. and Stanwood, P.C. (1989) Seed moisture: recalcitrant vs orthodox seeds. pp. 15–22 in Seed moisture. Crop Science Society of America, Special Publication, 14.Google Scholar
Dasgupta, J., Bewley, J.D. and Yeung, E.C. (1982) Desiccation tolerant and desiccation-intolerant stages during the development and germination of Phaseolus vulgaris seeds. Journal of Experimental Botany 33,1045–1057.CrossRefGoogle Scholar
De Ruiter, H., Schnurmans, J. and Kollöffel, C. (1984) Amino-acid leakage from cotyledons of developing and germinating pea seeds. Journal of Plant Physiology 116, 47–57.CrossRefGoogle ScholarPubMed
Duke, S.H., Kakefuda, G. and Harvey, T.M. (1983) Differential leakage of intracellular substances from imbibing soybean seeds. Plant Physiology 72, 919–924.CrossRefGoogle ScholarPubMed
Gautheret, R.J. (1959) La culture des tissus végétaux. Paris, France, Masson et Cie. 863 p.Google Scholar
Kakefuda, G. and Duke, S.H. (1982) Imbibitional leakage and seed death during germination in soybean cultivars with defective testae. Plant Physiology 69, S–1.Google Scholar
Kermode, A.R. and Bewley, J.D. (1985) The role of maturation drying in the transition from seed development to germination. I. Acquisition of desiccation-tolerance and germinability during development of Ricinus communis L. seeds. Journal of Experimental Botany 36,1906–1915.CrossRefGoogle Scholar
Kermode, A.R., Oishi, M.Y. and Bewley, J.D. (1989) Regulatory roles for desiccation and abscisic acid in seed development: a comparison of the evidence from whole seeds and isolated embryos. p. 23–50 in Seed Moisture, Crop Science Society of America, Special Publication, 14.Google Scholar
Le Page-Degivry, M.Th., Duval, D., Bulard, C. and Delaage, M. (1984) A radioimmunoassay for abscisic acid. Journal of Immunological Methods 67, 119–128.CrossRefGoogle Scholar
Le Page-Degivry, M.Th., Garello, G. and Barthe, Ph. (1990) Involvement of endogenous ABA in onset and release of Helianthus annuus embryo dormancy. Plant Physiology 92,1164–1168.CrossRefGoogle ScholarPubMed
Long, S.R., Dale, R.M.K. and Sussex, I.M. (1981) Maturation and germination of Phaseolus vulgaris embryonic axes in culture. Planta 153,405–415.CrossRefGoogle ScholarPubMed
Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
McKersie, B.D. and Stinson, R.H. (1980) Effect of dehydration on leakage and membrane structure in Lotus corniculatus L. seeds. Plant Physiology 66,316–320.CrossRefGoogle ScholarPubMed
Mohapatra, S.S., Poole, R.J. and Dhindsa, R.S. (1987) Cold acclimation, freezing resistance and protein synthesis in Alfalfa (Medicago sativa L. cv Saranac). Journal of Experimental Botany 38,1697–1703.CrossRefGoogle Scholar
Mohapatra, S.S., Poole, R.J. and Dhindsa, R.S. (1988) Detection of two membrane polypeptides induced by abscisic acid and cold acclimation. Possible role in freezing tolerance. Plant and Cell Physiology 29, 727–730.Google Scholar
Rachidian, Z. and Le Deunff, Y. (1986) Germination capacity of pea seeds. I. Pod and seed development: incidences of seed germination with and without drying. Agronomie 6, 463–468.CrossRefGoogle Scholar
Rogerson, N.E. and Matthews, S. (1977) Respiratory and carbohydrate changes in developing pea (Pisum sativum L.) seeds in relation to their ability to withstand desiccation. Journal of Experimental Botany 28, 304–313.CrossRefGoogle Scholar
Schonbeck, M.W. and Bewley, J.D. (1981) Response of the moss Tortula ruralis to desiccation treatments. I. Effects of minimum water content and rates of dehydration and rehydration. Canadian Journal of Botany 59,2698–2706.CrossRefGoogle Scholar
Senaratna, T., McKersie, B.D. and Bewley, J.D. (1989) Desiccation tolerance of alfalfa (Medicago sativa L) somatic embryos. Influence of abscisic acid, stress pretreatments and drying rates. Plant Science 65,253.Google Scholar