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Quantitative estimation of seed desiccation sensitivity using a quantal response model: application to nine species of the genus Coffea L.

Published online by Cambridge University Press:  22 February 2007

Stéphane Dussert ,
Nathalie Chabrillange ,
Florent Engelmann  and
Serge Hamon
Stéphane Dussert*
Affiliation:
ORSTOM, GeneTrop, BP 5045, 34032 Montpellier Cedex 1, France
Nathalie Chabrillange
Affiliation:
ORSTOM, GeneTrop, BP 5045, 34032 Montpellier Cedex 1, France
Florent Engelmann
Affiliation:
IPGRI, Via delle Sette Chiese 142, 00145 Rome, Italy
Serge Hamon
Affiliation:
ORSTOM, GeneTrop, BP 5045, 34032 Montpellier Cedex 1, France
*
*Correspondence Fax: +33 4 67 54 78 00 Email: dussert@orstom.fr
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Abstract

Seed desiccation sensitivity was studied in nine species of the genus Coffeaby measuring seed viability after equilibration over various saturated salt solutions. A quantal response model based on the logistic distribution was developed in order to describe the typical S-shaped patterns observed. The closeness of fit of the desiccation sensitivity model was shown, and the assumption that seed desiccation sensitivity follows a continuous distribution within species was verified. For each species, the water content at which 50% of initial viability was reached, WC50, and a specific parameter describing the intra-specific variability, β, were calculated using a non-linear regression. A simplified water sorption model was developed which allowed easy calculation of water activity and water potential corresponding to WC50 (aw50 and Ψ50) for relative humidities ranging between 10 and 100%. Distribution of WC50and Ψ50 (or aw50) in the genus Coffea was homogeneous within the following intervals: from 0.05 to 0.38 g H2O.g−1dw for WC50 and from −168 to −11 MPa for Ψ50. Different classifications of the coffee species studied as regards to their desiccation sensitivity were obtained depending on whether WC50 or Ψ50was used for classification. The continuum for desiccation sensitivity observed within the nine species studied confirmed that coffee is an appropriate material for studying desiccation sensitivity.

Keywords

Coffeaseeddesiccation sensitivity modelsorption isotherm
Type
Research Article
Information
Seed Science Research , Volume 9 , Issue 2 , February 1999 , pp. 135 - 144
Copyright
Copyright © Cambridge University Press 1999

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