Biologia plantarum 43:279-281, 2000 | DOI: 10.1023/A:1002768613872
Anti-Auxin Enhance Rosa Hybrida L. Micropropagation
- 1 Department of Horticulture, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi -, India
Shoots of rose cultivars Super Star and Sonia were multiplied for ten subcultures at 4-week intervals on solidified Murashige and Skoog's medium supplemented with 22.19 μM benzylaminopurine + 1.07 μM napthalene acetic acid + 0.05 μM gibberellic acid. Addition of anti-auxins 2,3,5-triiodobenzoic acid (TIBA; 2.0 μM) and 2,4,6-trichlorophenoxy-acetic acid (2,4,6-T; 0.39 μM) into proliferation medium increased number of shoots per explant and length of shoots in both cultivars. Treatment with TIBA increased also number of leaves per shoot and leaf chlorophyll content.
Keywords: axillary shoot proliferation; chlorophyll; hybrid tea rose; TIBA; 2,4,5-T
Subjects: anti-auxin, micropropagation; axilary shoot proliferation; chlorophyll, antiauxin; hybrid tea rose, micropropagation; in vitro culture, proliferation; Rosa hybrida; rose, micropropagation, growth regulators; 2,4,6-trichlorophenoxyacetic acid, micropropagation; 2,3,5-triiodobenzoid acid, micropropagation
Published: August 1, 2000 Show citation
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