Skip to main content
SpringerLink
Log in

Molecular breeding in the genus Musa: a strong case for STMS marker technology

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Musa species are among the tallest monocotyledons and include major food-producing species. The principal cultivars, derived from two major species Musa acuminata (‘A’ genome) and Musa balbisiana (‘B’ genome), are polyploid hybrids (mainly AAA, AAB and ABB triploids), medium to highly sterile, parthenocarpic and clonally propagated. Bananas and plantains are crops to which molecular breeding is expected to have a positive impact. In order to better understand banana genetics, more knowledge has to be accumulated about the complex genome structure of hybrids and cultivars. Therefore, the aim of our work is to develop molecular markers that are codominant, reliable, universal, highly polymorphic and that are applicable to collaborative Musa germplasm genotyping and mapping. Two size-selected genomic libraries have been screened for the presence of simple sequence repeats (SSR). Our data demonstrate that SSR are readily applicable to the study of Musa genetics. Our comprehensive analyses of a significant number of banana sequence tagged microsatellite sites (STMS) will add to our knowledge on the structure and phylogeny of genomes of the Musa species, and suggest that microsatellites be used as anchor markers for a banana genetic core map. Additional markers, such as e.g. CAPS have also been tested in order to increase the detection of polymorphisms exceeding that revealed by STMS technology. The utility of PCR-derived markers for collaborative genetic analyses of the banana genome, and the transferability of 'streamlined’ laboratory techniques and data analysis to Developing Countries are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Akkaya, M.S., R.C. Shoemaker, J.E. Specht, A.A. Bhagwat & P.B. Cregan, 1995. Integration of simple sequence repeat DNA markers into a soybean linkage map. Crop Sci 35: 1439–1445.

    Article  CAS  Google Scholar 

  • Ali, S., C.R. Müller & J.T. Epplen, 1986. DNA fingerprinting by oligonucleotide probes specific for simple repeats. Hum Genet 74: 239–243.

    Article  PubMed  CAS  Google Scholar 

  • Bassam, B.J., G. Caetano-Anollés & P.M. Gresshoff, 1991. Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196: 80–83.

    Article  PubMed  CAS  Google Scholar 

  • Becker, J. & M. Heun, 1995. Barley microsatellites: allele variation and mapping. Plant Mol Biol 27: 835–845.

    Article  PubMed  CAS  Google Scholar 

  • Bhat, K.V., R.L. Jarret & R.S. Rana, 1995a. DNA profiling of banana and plantain cultivars using random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) markers. Electrophoresis 16: 1736–1745.

    Article  PubMed  CAS  Google Scholar 

  • Bhat, K.V., S.R. Bhat, K.P.S. Chandel, S. Lakhanpaul & S. Ali, 1995b. DNA fingerprinting of Musa cultivars with oligodeoxyribonucleotide probes specific for simple repeat motifs. Genet Anal 12: 45–51.

    PubMed  CAS  Google Scholar 

  • Carreel, F., 1994. Etude de la diversité génétique des bananiers (genre Musa) à l'aide des marqueurs RFLP. Thesis. Institut national Agronomique Paris-Grignon, Paris, France.

    Google Scholar 

  • Carreel, F., S. Fauré, D. Gonzales de Leon, P.J.L. Lagoda, X. Perrier, F. Bakry, H. Tezenas du Montcel, C. Lanaud & J.P. Horry, 1994. Evaluation de la diversité génétique chez les bananiers diploïdes (Musa sp.). Genet Sel Evol 26: 125–130.

    Google Scholar 

  • Cheung, W.Y., G. Moore, T.A. Money & M.D. Gale, 1992. Hpa II library indicates ‘methylation-free islands’ in wheat and barley. Theor Appl Genet 84: 739–746.

    Article  Google Scholar 

  • Condit, R. & S.P. Hubbell, 1990. Abundance and DNA sequence of two-base repeat regions in tropical tree genomes. Genome 34: 66–71.

    Google Scholar 

  • Cregan, P.B., A.A. Bhagwat, M.S. Akkaya & J. Rongwen, 1994. Microsatellite mapping and fingerprinting in soybean. Meth Molec Cell Biol 5: 49–61.

    CAS  Google Scholar 

  • Delseny, M., M. Laroche, P. Penon, 1983. Detection of sequences with Z-DNA forming potential in higher plants. Biochem Biophys Res Commun 116: 113–120.

    Article  PubMed  CAS  Google Scholar 

  • Depeiges, A., C. Goubely, A. Lenoir, S. Cocherel, G. Picard, M. Raynal, F. Grellet & M. Delseny, 1995. Identification of the most represented repeated motifs in Arabidopsis thaliana microsatellite loci. Theor Appl Genet 91: 160–168.

    Article  CAS  Google Scholar 

  • Fauré, S., 1993. Contribution à la cartographie génétique du génome des bananiers diploïdes (Musa acuminata) à l'aide de marqueurs moléculaires. Thesis. Ecole Supérieure Agronomique de Rennes, Rennes, France.

    Google Scholar 

  • Fauré, S., J.-L. Noyer, J.-P. Horry, F. Bakry, C. Lanaud & D. Gonzales de Leon, 1993. A molecular marker-based linkage map of diploid bananas (Musa acuminata). Theor Appl Genet 87: 517–526.

    Article  Google Scholar 

  • Fauré, S., J.-L. Noyer, F. Carreel, J.-P. Horry, F. Bakry & C. Lanaud, 1994. Maternal inheritance of chloroplast genome and paternal inheritance of mitochondrial genome in bananas (Musa acuminata). Curr Genet 25: 265–269.

    Article  PubMed  Google Scholar 

  • Gawel, N.J. & R.L. Jarret, 1991. Chloroplast DNA restriction fragment length polymorphisms (RFLPs) in Musa species. Theor Appl Genet 81: 783–786.

    Article  CAS  Google Scholar 

  • Howell, E.C., H.J. Newbury, R.L. Swennen, L.A. Withers & B.V. Ford-Lloyd, 1993. The use of RAPD for identifying and classifying Musa germplasm. Genome 37: 328–332.

    Google Scholar 

  • Jarne, P. & P.J.L. Lagoda, 1996. Microsatellites, from molecules to populations and back. Trends Ecol Evol 11: 424–429.

    Article  Google Scholar 

  • Jarret, R.L., N.J. Gawel, A. Whittemore & S. Sharrock, 1992. RFLP-based phylogeny of Musa species in Papua New Guinea. Theor Appl Genet 84: 579–584.

    Article  Google Scholar 

  • Jarret, R.L., K.V. Bhat, P.B. Cregan, R. Ortiz & D.R. Vuylsteke, 1995. Isolation of microsatellite DNA markers in Musa. InfoMusa 3: 3–4.

    Google Scholar 

  • Jarret, R.L., T. Holms, J. Evans & L. Merrick, 1996. Simple sequence repeats in watermelon (Citrullus lanatus). Genome (in press).

  • Kaemmer, D., R. Afza, K. Weising, G. Kahl & F.J. Novak. Oligonucleotide and amplification fingerprinting of wild species and cultivars of banana (Musa spp.). Bio/Technology 10: 1030–1035.

  • Kijas, J.M.H., J.C.S. Fowler & M.R. Thomas, 1995. An evaluation of sequence-tagged microsatellite site markers for genetic analysis within Citrus and related species. Genome 38: 349–355.

    PubMed  CAS  Google Scholar 

  • Kresovich, S., A.K. Szewc-McFadden, S.M. Blieck & J.R. McFerson, 1995. Abundance and characterization of simple-sequence repeats (SSRs) isolated from a size-fractionated genomic library of Brassica napus L. (rapeseed). Theor Appl Genet 91: 206–211.

    Article  CAS  Google Scholar 

  • Konieczny, A. & F.M. Ausubel, 1993. A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers. Plant J 4: 403–410.

    Article  PubMed  CAS  Google Scholar 

  • Lagoda, P.J.L., J.L. Noyer, D. Dambier, F.C. Baurens & C. Lanaud, 1995. Abundance and distribution of SSR (simple sequence repeats) in the Musaceae family: Microsatellite markers to map the banana genome. Proceedings of the FAO/IAEA Symposium on Induced mutations and molecular techniques for crop improvement (Vienna, 19–23 june), IAEA-SM-340/016: 287–295.

  • Lagoda, P. & J.L. Noyer, 1995. Update on Musa genome mapping at CIRAD-AGETROP. InfoMusa 3: 4.

    Google Scholar 

  • Lagercrantz, U., H. Ellegren & L. Andersson, 1993. The abundance of various polymorphic microsatellite motifs differs between plants and vertebrates. Nucl Acids Res 21: 1111–1115.

    PubMed  CAS  Google Scholar 

  • Lavi, U., M. Akkaya, A.A. Bhagwat, E. Lahav & P.B. Cregan, 1994. Methodology of generation and characteristics of simple sequence repeat DNA markers in avocado (Persea americana M.). Euphytica 80: 171–177.

    Article  CAS  Google Scholar 

  • Liu, Z.-W., R.L. Jarret, S. Kresovich & R.R. Duncan, 1995. Characterization and analysis of simple sequence repeat (SSR) loci in seashore paspalum (Paspalum vaginatum Swartz). Theor Appl Genet 91: 47–52.

    CAS  Google Scholar 

  • Maughan, P.J., M.A. Sahai Maroof & G.R. Buss, 1995. Microsatellite and amplified sequence length polymorphisms in cultivated and wild soybean. Genome 38: 715–723.

    PubMed  CAS  Google Scholar 

  • Morgante, M. & A.M. Olivieri, 1993. PCR-amplified microsatellites as markers in plant genetics. Plant J 3: 175–182.

    Article  PubMed  CAS  Google Scholar 

  • Novak, F.J., 1992. Musa (Bananas and Plantains). In: F.A. Hammerschlag & R.E. Litz (Eds) Biotechnology of Perennial Fruit Crops. CAB International, Wallingford, UK, pp. 449–488.

    Google Scholar 

  • Plaschke, J., M.W. Ganal & M.S. Röder, 1995. Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor Appl Genet 91: 1001–1007.

    Article  CAS  Google Scholar 

  • Röder, M.S., J. Plaschke, S.U. König, A. Börner, M.E. Sorrells, S.D. Tanksley & M.W. Ganal, 1994. Abundance, variability and chromosomal location of microsatellites on wheat. Mol Gen Genet 246: 327–333.

    Article  Google Scholar 

  • Rongwen, J., M.S. Akkaya, A.A. Bhagwat, U. Lavi & P.B. Cregan, 1995. The use of microsatellite DNA markers for soybean genotype identification. Theor Appl Genet 90: 43–48.

    Article  CAS  Google Scholar 

  • Saghai Maroof, M.A., R.M. Biyashev, G.P. Yang, Q. Zhang & R.W. Allard, 1994. Extraordinarily polymorphic microsatellite DNA in barley: Species diversity, chromosomal locations, and population dynamics. Proc Natl Acad Sci 91: 5466–5470.

    Article  PubMed  CAS  Google Scholar 

  • Sambrook, J., E.F. Fritsch & T. Maniatis, 1989. Molecular Cloning. A laboratory manual. Cold Spring Harbour Laboratory Press, NY.

    Google Scholar 

  • Schierwater, B. & A. Ender, 1993. Different thermostable DNA polymerases may amplify different RAPD products. Nucl Acids Res 21: 4647–4648.

    PubMed  CAS  Google Scholar 

  • Senior, M.L. & M. Heun, 1993. Mapping maize microsatellites and polymerase chain reaction confirmation of the targeted repeats using a CT primer. Genome 36: 884–889.

    PubMed  CAS  Google Scholar 

  • Stover, R.H. & N.W. Simmonds, 1987. Bananas. (Third edition), Longman Scientific & Technical, New York, USA.

    Google Scholar 

  • Tautz, D. & M. Renz, 1984. Simple sequences are ubiquitous repetitive components of eucaryotic genomes. Nucl Acids Res 12: 4127–4138.

    PubMed  CAS  Google Scholar 

  • Terauchi, R. & A. Konuma, 1994. Microsatellite polymorphism in Dioscorea tokoro, a wild yam species. Genome 37: 794–801.

    PubMed  CAS  Google Scholar 

  • Weising, K., R.W.M. Fung, D.J. Keeling, R.G. Atkinson & R.C. Gardner, 1996. Characterisation of microsatellites from Actinidia chinensis. Mol Breed (in press).

  • Winter, P. & G. Kahl, 1995. Molecular marker technologies for plant improvement. World J Microbiol Biotechnol 11: 438–448.

    Article  CAS  Google Scholar 

  • Wu, K.S. & S.D. Tanksley, 1993. Abundance, polymorphism and genetic mapping of microsatellites in rice. Mol Gen Genet 241: 225–235.

    Article  PubMed  CAS  Google Scholar 

  • Yang, G.P., M.A. Saghai Maroof, C.G. Xu, Q. Zhang & R.M. Biyashev, 1994. Comparative analysis of microsatellite DNA polymorphism in landraces and cultivars of rice. Mol Gen Genet 245: 187–194.

    Article  PubMed  CAS  Google Scholar 

  • Zhang, Q., Y.J. Gao, M.A. Saghai Maroof, S.H. Yang & J.X. Li, 1995 Molecular divergence and hybrid performance in rice. Mol Breed 1: 133–142.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Plant Molecular Biology, Biozentrum, Johann Wolfgang Goethe-University, Marie-Curie-Str. 9, D-60439, Frankfurt am Main, Germany

    D. Kaemmer & D. Fischer

  2. Usda/ars, Plant Genetic Resources, 1109 Experiment Street, Griffin, Georgia, 30223, USA

    R.L. Jarret

  3. Cirad-Gerdat, Biotrop Research Unit, AGETROP Laboratory, BP 5035, F-34032, Montpellier, Cedex 1, France

    F.-C. Baurens, A. Grapin, D. Dambier, J.-L. Noyer, C. Lanaud & P.J.L. Lagoda

  4. Plant Molecular Biology, Biozentrum, Johann Wolfgang Goethe-University, Marie-Curie-Str. 9, D-60439, Frankfurt am Main, Germany

    G. Kahl

Authors
  1. D. Kaemmer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. Fischer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R.L. Jarret
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. F.-C. Baurens
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Grapin
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. D. Dambier
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. J.-L. Noyer
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. C. Lanaud
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. G. Kahl
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. P.J.L. Lagoda
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kaemmer, D., Fischer, D., Jarret, R. et al. Molecular breeding in the genus Musa: a strong case for STMS marker technology. Euphytica 96, 49–63 (1997). https://doi.org/10.1023/A:1002922016294

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1002922016294

Search

Navigation