Skip to main content
SpringerLink
Log in

Biochemical, molecular, and cytogenetic technologies for characterizing 1RS in wheat: A review

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Chromosome arm 1RS of rye ( Secale cereale L.), when transferred to wheat ( Triticum sp.), significantly influences variety performance, because it carries genes for resistance to disease and insect pathogens. Inserted into wheat, 1RS also promotes haploid production, affects end-product quality, and sometimes affects yield. Therefore, its detection by breeders and geneticists is important. The entire 1RS arm is present in chromosome substitutions and in Robertsonian translocations involving chromosomes 1A, 1B, or 1D of wheat. In recombinant lines, a segment of 1RS has been exchanged with a segment of a group-1 wheat chromosome. Determining the wheat chromosome arm involved in a translocation, the source of rye chromatin, and the amount of 1RS chromatin introduced is necessary for a complete characterization of the introgressed segment. Biochemical, molecular, and cytogenetic technologies are described which enable such a characterization of 1RS in wheat. Examples of using gel electrophoresis, high-performance liquid chromatography, monoclonal antibodies, rye-specific molecular probes, RFLP and PCR assays, chromosome banding, in situ hybridization, and flow cytometry are provided. A comparison of these technologies is made and the advantages and disadvantages of each technology are discussed relative to modern wheat breeding efforts.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abbo, S., T.E. Miller & I.P. King, 1993. Primer-induced in situ hybridization to plant chromosomes. Genome 36: 815–817.

    PubMed  CAS  Google Scholar 

  • Anderson, J.A., Y. Ogihara, M.E. Sorrells & S.D. Tanksley, 1992. Development of a chromosomal arm map for wheat based on RFLP markers. Theor Appl Genet 83: 1035–1043.

    CAS  Google Scholar 

  • Andrews, J.L., M.J. Blundell & J.H. Skerritt, 1996. Differentiation of wheat-rye translocation lines using antibody probes for Gli-B1 and Sec-1. J Cereal Sci 23: 61–72.

    Article  Google Scholar 

  • Appels, R. & E.S. Lagudah, 1990. Manipulation of chromosomal segments from wild wheat for the improvement of bread wheat. Aust J Plant Physiol 17: 253–266.

    Google Scholar 

  • Appels, R., L.B. Moran & J.P. Gustafson, 1986. Rye heterochromatin. I. Studies on clusters of the major repeating sequence and the identification of a new dispersed repetitive sequence element. Can J Genet Cytol 28: 645–657.

    CAS  Google Scholar 

  • Autran, J.-C., 1993. Recent perspectives on the genetics, biochemistry and functionality of wheat proteins. Trends Food Sci Tech 4: 358–364.

    Google Scholar 

  • Bartos, P., J. Valkoun, J. Kosner & V. Slovencikova, 1973. Rust resistance of some European wheat cultivars derived from rye. 4th Int. Wheat Genet. Symp., Columbia, MO, USA. pp. 145–146.

  • Bashir, A., J.A. Auger & A.L. Rayburn, 1993. Flow cytometric DNA analysis of wheat-rye addition lines. Cytometry 14: 843–847.

    Article  PubMed  CAS  Google Scholar 

  • Baum, M. & R. Appels, 1991. The cytogenetic and molecular architecture of chromosome 1R — one of the most widely utilized sources of alien chromatin in wheat varieties. Chromosoma 101: 1–10.

    Article  PubMed  CAS  Google Scholar 

  • Bedo, Z., L. Balla, L. Szunics, I. Karsai & L. Lang, 1993. Agronomic and in vitro androgenetic variability of wheat cultivars with 1B/1R. 8th Int. Wheat Genet. Symp., Beijing, China. pp. 1225–1229.

  • Bennett, M.D. & J.B. Smith, 1975. Confirmation of the identification of the rye chromosome in 1B/1R wheat-rye chromosome substitution and translocation lines. Can J Genet Cytol 17: 117–120.

    Google Scholar 

  • Berzonsky, W.A., Clements, R.L. & H.N. Lafever, 1991. Identification of ‘Amigo’ and ‘Kavkaz’ translocations in Ohio soft red winter wheats (Triticum aestivum L.). Theor Appl Genet 81: 629–634.

    Article  Google Scholar 

  • Bluthner, W.-D. & D. Mettin, 1977. Chromosomen substitutionen und-translokationen zwischen weizen und roggen und deren bedeutung fur die zuchtung. Arch Zuchtungsforsch, Berlin 7: 15–27.

    Google Scholar 

  • Bringezu, Th., R.-G. Kynast, U. Vahl, & G. Muller. 1998a. Wheat lines with a set of three disomic wheat-rye 1RS translocations from different rye origin. 9th Int. Wheat Genet. Symp., Vol. 2, Saskatoon, Saskatchewan, Canada. pp. 22–25.

    Google Scholar 

  • Bringezu, Th., U. Vahl, & G. Muller, 1998b. Detection of four 1RS (Insave)-wheat-rye translocations in doubled haploid winter wheat lines. In: T. Lelley (Ed), Current topics in plant cytogenetics related to plant improvement. WUV-Univ.-Verl., Austria. pp. 325–333.

    Google Scholar 

  • Bullrich, L., G. Tranquilli, L.A. Pfluger, E.Y. Suarez & A.J. Barneix, 1998. Bread-making quality and yield performance of 1BL/1RS wheat isogenic lines. Plant Breed 117: 119–122.

    Article  CAS  Google Scholar 

  • Busch, W., R.G. Herrmann, & R. Martin, 1995. Refined physical mapping of the Sec-1 locus on the satellite of chromosome 1R of rye (Secale cereale). Genome 38: 889–893.

    CAS  PubMed  Google Scholar 

  • Cai, X., 1994. Chromosome translocations in the common wheat variety ‘Amigo’. Hereditas 121: 199–202.

    Article  Google Scholar 

  • Carver, B.F. & A.L. Rayburn, 1994. Comparison of related wheat stocks possessing 1B or 1RS.1BL chromosomes: agronomic performance. Crop Sci 34: 1505–1510.

    Article  Google Scholar 

  • Chojecki, A.J.S. & M.D. Gale, 1982. Genetic control of glucose phosphate isomerase in wheat and related species. Heredity 49: 337–347.

    CAS  Google Scholar 

  • Clarke, B.C., L.B. Moran & R. Appels, 1989. DNA analyses in wheat breeding. Genome 32: 334–339.

    Google Scholar 

  • Clarke, B.C., Y. Mukai & R. Appels, 1996. The Sec-1 locus on the short arm of chromosome 1R of rye (Secale cereale). Chromosoma 105: 269–275.

    Article  PubMed  CAS  Google Scholar 

  • Clements, R.L., 1988. A labor-saving technique for polyacrylamide gel electrophoresis of gliadins from large numbers of single wheat kernels. Cereal Chem 65: 150–152.

    CAS  Google Scholar 

  • Darling, D.C. & P.M. Brickell, 1994. Nucleic acid blotting: The basics. Oxford U. Press, NY, NY, USA.

    Google Scholar 

  • Dawood, M.R., N.K. Howes & W. Bashuk, 1989. Preparation of monoclonal antibodies against specific gliadin proteins and preliminary investigation of their ability to discriminate cereal cultivars. J Cereal Sci 10: 105–112.

    CAS  Google Scholar 

  • de Froidmont, D. 1998. A co-dominant marker for the 1BL/1RS wheat-rye translocation via multiplex PCR. J Agri Sci 27: 229–232.

    CAS  Google Scholar 

  • Delaney, D.E., B.R. Friebe, J.H. Hatchett, B.S. Gill & S.H. Hulbert, 1995. Targeted mapping of rye chromatin in wheat by representational difference analysis. Genome 38: 458–466.

    CAS  PubMed  Google Scholar 

  • Devos, K. & M. Gale, 1993. The genetic maps of wheat and their potential in plant breeding. Outlook on Agric 22: 93–99.

    Google Scholar 

  • Dhaliwal, A.S. & F. MacRitchie, 1990. Contributions of protein fractions to dough handling properties of wheat-rye translocation cultivars. J Cereal Sci 12: 113–122.

    CAS  Google Scholar 

  • Dhaliwal, A.S., D.J. Mares, D.R. Marshall & J.H. Skerritt, 1988. Protein composition and pentosan content in relation to dough stickiness of 1B/1R translocation wheats. Cereal Chem 65: 143–149.

    CAS  Google Scholar 

  • Dolezel, J., S. Lucretti & I. Schubert, 1994. Plant chromosome analysis and sorting by flow cytometry. Crit Rev Plant Sci 13: 275–309.

    Google Scholar 

  • Ellis, H.J., A.R. Freedman & P.J. Ciclitira, 1989. The production and characterisation of monoclonal antibodies to wheat gliadin peptides. J Immun Methods 120: 17–22.

    Article  CAS  Google Scholar 

  • Fenn, D., O.M. Lukow, W. Bushuk & R.M. DePauw, 1994. Milling and baking quality of 1BL/1RS translocation wheats. I. Effects of genotype and environment. Cereal Chem 71: 189–195.

    Google Scholar 

  • Fernandez-Calvin, B., E. Benavente & J. Orellana, 1995. Meiotic pairing in wheat-rye derivatives detected by genomic in situ hybridization and C-banding-a comparative analysis. Chromosoma 103: 554–558.

    PubMed  CAS  Google Scholar 

  • Francis, H.A., A.R. Leitch & R.M.D. Koebner, 1995. Conversion of a RAPD-generated PCR product, containing a novel dispersed repetitive element, into a fast and robust assay for the presence of rye chromatin in wheat. Theor Appl Genet 90: 636–642.

    Article  Google Scholar 

  • Gale, M.D. & T.E. Miller, 1987. The introduction of alien variation into wheat. In: F.G.H. Lupton (Ed), Wheat breeding: its scientific basis, Chapman and Hall Publ., NY,NY, USA. pp. 173–210.

    Google Scholar 

  • Gill, B.S., 1993. Molecular cytogenetics: integration of C-banding, in situ hybridization, and RFLP's in genome mapping and crop improvement of wheat. 8th Int. Wheat Genet. Symp., Beijing, China. pp. 41–47.

  • Gill, K.S., B.S. Gill, T.R. Endo & T. Taylor, 1996. Identification and high-density mapping of gene-rich regions in chromosome group 1 of wheat. Genetics 144: 1883–1891.

    PubMed  CAS  Google Scholar 

  • Gill, B.S. & G. Kimber, 1977. Recognition of translocations and alien chromosome transfers in wheat by the geimsa C-banding technique. Crop Sci. 17: 264–266.

    Article  Google Scholar 

  • Gill, B.S., F. Lu, R. Schlegel & T.R. Endo, 1988. Toward a cytogenetic and molecular description of wheat chromosomes. 7th Int. Wheat Genet. Symp., Cambridge, UK. pp. 477–481.

  • Gosden, J.R., 1997. PRINS and in situ PCR protocols. Humana Press, New Jersey, USA.

    Google Scholar 

  • Graybosch, R.A., C.J. Peterson, L.E. Hansen, D. Worrall, D.R. Shelton & A. Lukaszewski, 1993a. Comparative flour quality and protein characteristics of 1BL/1RS and 1AL/1RS wheat rye translocation lines. J Cereal Sci 17: 95–106.

    Article  CAS  Google Scholar 

  • Graybosch, R.A., Y.W. Seo & C.J. Peterson, 1993b. Detection of wheat-rye chromosomal translocations using an antisecalin monoclonal antibody. Cereal Chem 70: 458–463.

    CAS  Google Scholar 

  • Greulich, K.O., 1992. Chromosome microtechnology: microdissection and microcloning. Trends Biotech 10: 48–51.

    Article  CAS  Google Scholar 

  • Guidet, F., 1994. A powerful new technique to quickly prepare hundreds of plant extracts for PCR and RAPD analyses. Nucl Acids Res 22: 1772–1773.

    PubMed  CAS  Google Scholar 

  • Guidet, F., P. Rogowsky, C. Taylor, W. Song & P. Langridge, 1991. Cloning and characterisation of a new rye-specific repeated sequence. Genome 34: 81–87.

    Google Scholar 

  • Gupta, R.B. & K.W. Shepherd, 1992. Identification of rye chromosome 1R translocations and substitutions in hexaploid wheats using storage proteins as genetic markers. Plant Breed. 109: 130–140.

    Article  CAS  Google Scholar 

  • Gupta, R.B. & K.W. Shepherd, 1993. Production of multiple wheatrye translocation stocks and genetic analysis of LMW subunits of glutenin and gliadins in wheats using these stocks. Theor Appl Genet 85: 719–728.

    Article  CAS  Google Scholar 

  • Hanusova, R., S.L.K. Hsam, P. Bartos & F. Zeller. 1996. Suppression of powdery mildew resistance gene Pm8 in Triticum aestivum L. (common wheat) cultivars carrying wheat-rye translocation T1BL.1RS. Heredity 77: 383–387.

    Google Scholar 

  • Hartmann, H., S. Schiele & T. Lelley, 1994. Isoenzyme electrophoresis, a simple way to identify 1B/1R substitutions and translocations in wheat. Plant Breed 112: 338–341.

    Article  Google Scholar 

  • Henry, Y., S. Bernard, M. Bernard, G. Gay, J.-L. Marcotte & J. De Buyser, 1993. Nuclear gametophytic genes from chromosome arm 1RS improve regeneration of wheat microspore-derived microspore-derived embryos. Genome 36: 808–814.

    CAS  PubMed  Google Scholar 

  • Heslop-Harrison, J.S., 1991. The molecular cytogenetics of plants. J Cell Sci 100: 15–21.

    CAS  Google Scholar 

  • Heslop-Harrison, J.S., A.R. Leitch, T. Schwarzacher & K. Anamthawat-Jonsson, 1990. Detection and characterization of 1B/1R translocation in hexaploid wheat. Heredity 65: 385–392.

    Google Scholar 

  • Heun, M. & B. Friebe, 1990. Introgression of powdery mildew resistance from rye into wheat. Phytopath 80: 242–245.

    Google Scholar 

  • Heun, M., A.E. Kennedy, J.A. Anderson, N.L.V. Lapitan, M.E. Sorrells & S.D. Tanksley, 1991. Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34: 437–447.

    Google Scholar 

  • Holmquist, G.P. & M.A. Motara, 1987. The magic of cytogenetic technology. In: G. Obe & A. Basler (Eds), Cytogenetics, Springer-Verlag, Berlin, Germany. pp. 30–47.

    Google Scholar 

  • Hou, G., H. Yamamoto & P.K.W. Ng, 1996. Relationships of quantity of gliadin subgroups of selected U.S. soft wheat flours to rheological and baking properties. Cereal Chem 73: 352–357.

    CAS  Google Scholar 

  • Howes, N.K, O.M. Lukow, M.R. Dawood & W. Bashuk, 1989. Rapid detection of the 1BL/1RS chromosome translocation in hexaploid wheats by monoclonal antibodies. J Cereal Sci 10: 1–4.

    Google Scholar 

  • Hsam, S.L.K., M.-C. Cermeno, B. Friebe & F.J. Zeller, 1995. Transfer of Amigo wheat powdery mildew resistance Pm17 from T1AL.1RS to the T1BL.1RS wheat-rye translocated chromosome. Heredity 74: 497–501.

    Google Scholar 

  • Hsam, S.L.K. & F.J. Zeller, 1993. Haploid production in durum wheat by the interaction of Aegilops kotschyi cytoplasm and 1BL/1RS chromosomal interchange. Theor Appl Genet 86: 951–954.

    Article  Google Scholar 

  • Hsam, S.L.K. & F.J. Zeller. 1997. Evidence of allelism between genes Pm8 and Pm17 and chromosomal location of powdery mildew and leaf rust resistance genes in the common wheat cultivar ‘Amigo’. Plant Breed 116: 119–122.

    Article  Google Scholar 

  • Hussain, A. & O.M. Lukow, 1994. Characterization of the 1B.1R translocation in wheat using water extractable protein concentrate. Euphytica 78: 108–113.

    Google Scholar 

  • Iqbal, M.J. & A.L. Rayburn. 1995. Identification of the 1RS rye chromosomal segment in wheat by RAPD analysis. Theor Appl Genet 91: 1048–1053.

    Article  CAS  Google Scholar 

  • Islam, A.K.M.R. & K.W. Shepherd, 1991. Alien genetic variation in wheat improvement. In: P.K. Gupta & T. Tsuchiya (Eds), Chromosome engineering in plants: genetics, breeding, evolution, Part A, Elsevier Publ., NY, NY, USA. pp. 291–312.

    Google Scholar 

  • Islam-Faridi, M.N. & A. Mujeeb-Kazi, 1995. Visualization of Secale cereale DNA in wheat germplasm by fluorescent in situ hybridization. Theor Appl Genet 90: 595–600.

    Article  Google Scholar 

  • Javornik, B., T. Sinkovic, L. Vapa, R.M.D. Koebner & W.J. Rogers, 1991. A comparison of methods for identifying and surveying the presence of 1BL.1RS translocations in bread wheat. Euphytica 54: 45–53.

    Article  CAS  Google Scholar 

  • Jiang, J. & B.S. Gill, 1993. Sequential chromosome banding and in situ hybridization analysis. Genome 36: 792–795.

    PubMed  CAS  Google Scholar 

  • Jiang, J. & B.S. Gill, 1994. Nonisotopic in situ hybridization and plant genome mapping: the first 10 years. Genome 37: 717–725.

    CAS  PubMed  Google Scholar 

  • Jiang, J., B. Friebe & B.S. Gill, 1994a. Chromosome painting of Amigo wheat. Theor Appl Genet 89: 811–813.

    Article  CAS  Google Scholar 

  • Jiang, J., B. Friebe & B.S. Gill, 1994b. Recent advances in alien gene transfer in wheat. Euphytica 73: 199–212.

    Article  Google Scholar 

  • Jones, B.L., G.L. Lookhart, S.B. Hall & K.F. Finney. 1982. Identification of wheat cultivars by gliadin electrophoresis: electrophoregrams of the 88 wheat cultivars most commonly grown in the U.S. in 1979. Cereal Chem 59: 181–188.

    CAS  Google Scholar 

  • Jouve, N., A. Bernardo, A. Cuadrado, A. De Bustos, P. Rubio & C. Soler. 1998. Detection of alien introgression into cultivated cereals using cytogenetic and molecular techniques. In: T. Lelley (Ed), Current topics in plant cytogenetics related to plant improvement. WUV-Univ.-Verl., Austria pp. 33–42.

    Google Scholar 

  • Kazman, M.E., V. Lein & G. Robbelen, 1998. The 1BL.1RS translocation in recently developed European wheats. In: T. Lelley (Ed), Current topics in plant cytogenetics related to plant improvement. WUV-Univ.-Verl., Austria pp. 334–341.

    Google Scholar 

  • Kobayashi, M. & K. Tsunewaki, 1978. Haploid induction and its genetic mechanism in alloplasmic common wheat. J Heredity 71: 9–14.

    Google Scholar 

  • Koebner, R.M.D., 1990. Subtilisin inhibitor-a polymorphic protein produced by a gene on the short arms of wheat homoeologous group 1 chromosomes. J Genet Breed 44: 49–52.

    Google Scholar 

  • Koebner, R.M.D., 1995. Generation of PCR-based markers for the detection of rye chromatin in a wheat background. Theor Appl Genet 90: 740–745.

    Article  Google Scholar 

  • Koebner, R.M.D. & K.W. Shepherd, 1988. Isolation and agronomic assessment of allosyndetic recombinants derived from wheat/rye translocation 1DL.1RS, carrying reduced amounts of rye chromatin. 7th Int. Wheat Genet. Symp., Cambridge, UK. pp. 343–348.

  • Koebner, R.M.D., K.W. Shepherd & R. Appels, 1986. Controlled introgression to wheat of genes from rye chromosome arm 1RS by induction of allosyndesis. 2. Characterisation of recombinants. Theor Appl Genet 73: 209–217.

    Article  CAS  Google Scholar 

  • Korzun, V., S. Malyshev, N. Kartel, T. Westermann, W.E. Weber & A. Borner, 1998. A genetic linkage map of rye (Secale cereale L.). Theor Appl Genet 96: 203–208.

    Article  CAS  Google Scholar 

  • Lange, D.A., S. Penuela, R.L. Denny, J. Mudge, V.C. Concibido, J.H. Orf & N.D. Young, 1998. A plant DNA isolation protocol suitable for polymerase chain reaction based marker-assisted breeding. Crop Sci 38: 217–220.

    Article  CAS  Google Scholar 

  • Lange, W., 1988. Cereal cytogenetics in retrospect. What came true of some cereal cytogeneticist's pipe dreams? Euphytica S: 7–25.

  • Lapitan, N.L.V., R.G. Sears, A.L. Rayburn & B.S. Gill, 1986. Wheat-rye translocations. Detection of chromosome breakpoints by in situ hybridization with a biotin-labeled DNA probe. J Heredity 77: 415–419.

    Google Scholar 

  • Lavania, U.C., 1998. Fluorescence in situ hybridization in genome, chromosome and gene identification in plants. Current Sci 74: 126–133.

    CAS  Google Scholar 

  • Law, C.N., J.W. Snape & A.J. Worland, 1987. Anueploidy in wheat and its uses in genetic analysis. In: F.G.H. Lupton (Ed), Wheat breeding: its scientific basis, Chapman & Hall, London, UK. pp. 71–108.

    Google Scholar 

  • Lawrence, G.J. & R. Appels, 1986. Mapping the nucleolus organizer region, seed protein loci and isozyme loci on chromosome 1R in rye. Theor Appl Genet 71: 742–749.

    Article  CAS  Google Scholar 

  • Lawrence, G.J. & K.W. Shepherd, 1981. Chromosomal location of genes controlling seed proteins in species related to wheat. Theor Appl Genet 59: 25–31.

    CAS  Google Scholar 

  • Le, H.T. & K.C. Armstrong, 1991. In situ hybridization as a rapid means to assess meiotic pairing and detection of alien DNA transfers in interphase cells of wide crosses involving wheat and rye. Mol Gen Genet 225: 33–37.

    Article  PubMed  CAS  Google Scholar 

  • Le, H.T., K.C. Armstrong & B. Miki, 1989. Detection of rye DNA in wheat-rye hybrids and wheat translocation stocks using total genomic DNA as probe. Plant Mol Biol Rep 7: 150–158.

    Google Scholar 

  • Lee, J.-H., K. Arumuganathan, Y. Yen, S. Kaeppler, H. Kaeppler & P.S. Baenziger, 1997. Root tip cell synchronization and metaphase-chromosome isolation suitable for flow sorting in common wheat (Triticum aestivum L.). Genome 40: 633–638.

    PubMed  CAS  Google Scholar 

  • Leitch, I.J., A.R. Leitch & J.S. Heslop-Harrison, 1991. Physical mapping of plant DNA sequences by simultaneous in situ hybridization of two differently labeled fluorescent probes. Genome 34: 329–333.

    Google Scholar 

  • Lelley, T. & R. Bothe, 1993. The effect of a complete 1B/1R substitution on quantitative traits in wheat. 8th Int. Wheat Genet. Symp., Beijing, China. pp. 233–235.

  • Lisitsyn, N., N. Lisitsyn & M. Wigler, 1993. Cloning the differences between two complex genomes. Science 259: 946–951.

    PubMed  CAS  Google Scholar 

  • Lookhart, G.L., R. Graybosch, J. Peterson & A. Lukaszewski, 1991. Identification of wheat lines containing the 1BL/1RS translocation by high-performance liquid chromatography. Cereal Chem 68: 312–316.

    CAS  Google Scholar 

  • Lukaszewski, A., 1990. Frequency of 1RS.1AL and 1RS.1BL translocations in United States wheats. Crop Sci 30: 1151–1153.

    Article  Google Scholar 

  • Lukaszewski, A., 1993. Reconstruction in wheat of complete chromosomes 1B and 1R from the 1RS.1BL translocation of ‘Kavkaz’ origin. Genome 36: 821–824.

    CAS  PubMed  Google Scholar 

  • Lukaszewski, A., 1997. Further manipulation by centric misdivision of the 1RS.1BL translocation in wheat. Euphytica 94: 257–261.

    Article  Google Scholar 

  • Mansfield, D.C., A.F. Brown, D.K. Green, A.D. Corothers, S.W. Morris, H.J. Evans & A.F. Wright, 1994. Automation of genetic linkage analysis using fluorescent microsatellite markers. Genomics 24: 225–233.

    Article  PubMed  CAS  Google Scholar 

  • Mantzaris, G.J., W.M.C. Rosenberg & D.P. Jewell, 1990. The immunology of coeliac disease. Springer Semin. Immunopath 12: 219–229.

    CAS  Google Scholar 

  • Marais, G.F., M. Horn & F. Du Toit, 1994. Intergeneric transfer (rye to wheat) of a gene(s) for Russian wheat aphid resistance. Plant Breed 113: 265–271.

    Article  Google Scholar 

  • Marchylo, B.A., K.A. Handel & V.J. Mellish, 1989. Fast horizontal sodium dodecyl sulfate gradient polyacrylamide gel electrophoresis for rapid wheat cultivar identification and analysis of high molecular weight glutenin subunits. Cereal Chem 66: 186–192.

    CAS  Google Scholar 

  • Martin, T.J., T.L. Harvey & R.W. Livers, 1976. Resistance to wheat streak mosaic virus and its vector, Aceria tulipae. Phytopath 66: 346–349.

    Google Scholar 

  • Martin, D.J. & B.G. Stewart, 1990. Dough stickiness in rye-derived wheat cultivars. Euphytica 51: 77–86.

    Article  Google Scholar 

  • May, C.E. & F. Wray, 1991. A rapid technique for the detection of wheat-rye translocation chromosomes. Genome 34: 486–488.

    Google Scholar 

  • McIntyre, C.L., S. Pereira, L.B. Moran & R. Appels, 1990. New Secale cereale (rye) DNA derivatives for the detection of rye chromosome segments in wheat. Genome 33: 635–640.

    PubMed  CAS  Google Scholar 

  • McKendry, A.L., D.N. Tague, P.L. Finney & K.E. Miskin, 1996. Effect of 1BL.1RS on milling and baking quality of soft red winter wheat. Crop Sci 36: 848–851.

    Article  Google Scholar 

  • Merker, A., 1982. 'Veery'-a CIMMYT spring wheat with the 1B/1R chromosome translocation—. Cereal Res Commun 10: 105–106.

    Google Scholar 

  • Merker, A., 1984. The rye genome in wheat breeding. Hereditas 100: 183–191.

    Google Scholar 

  • Mettin, D. & W.D. Bluthner, 1996. The development of cytogenetic research at the Plant Breeding Institute Halle/Hohenthurm with 18 special reference to aneuploidy in cereals. Euphytica 89: 125–141.

    Article  Google Scholar 

  • Mettin, D., W.D. Bluthner & G. Schlegel, 1973. Additional evidence on spontaneous 1B/1R wheat-rye substitutions and translocations. 4th Int. Wheat Genet. Symp., Columbia, MO, USA. pp. 179–184.

  • Mettin, D., W.D. Bluthner & M. Weinrich, 1978. Studies on the nature and possible origin of the spontaneously translocated 1B-1R chromosome in wheat. Wheat Inf Serv 47, 48: 12–16.

    Google Scholar 

  • Meyer, V.R., 1988. Practical high-performance liquid chromatography. J. Wiley & Sons, NY, NY, USA.

    Google Scholar 

  • Miller, T.E., S.M. Reader, K.A. Purdie, S. Abbo, R.P. Dunford & I.P. King, 1995. Fluorescent in situ hybridization as an aid to introducing alien genetic variation into wheat. Euphytica 85: 275–279.

    Article  Google Scholar 

  • Millet, E. & M. Feldman, 1993. Deletion of the secalin gene Sec-1 in 1BL/1RS line by-irradiation. 8th Int. Wheat Genet. Symp., Beijing, China. pp. 851–854.

  • Moonen, J.H.E. & A.C. Zeven, 1984. SDS-PAGE of the highmolecular-weight subunits of wheat glutenin and the characterization of 1R(1B) substitution and 1BL/1RS translocation lines. Euphytica 33: 3–8.

    Article  CAS  Google Scholar 

  • Mujeeb-Kazi, A. & J.L. Miranda, 1985. Enhanced resolution of somatic chromosome constrictions as an aid to identifying intergeneric hybrids among some Triticeae. Cytologia 50: 701–709.

    Google Scholar 

  • Mujeeb-Kazi, A., M.D.H.M. William & M.N. Islam-Faridi, 1996. Homozygous 1B and 1BL/1RS chromosome substitutions in Triticum aestivum and T. turgidum cultivars. Cytologia 61: 147–154.

    Google Scholar 

  • Muller, G. & U. Vahl, 1986. Vergleich elektrophoretischer peroxydasemuster von 1A-1R und 1B-1R weizen-roggentranslokationsformen. Biochem Physiol. Pflanzen 181: 425–429.

    Google Scholar 

  • Nkongolo, K.K., N.L.V. Lapitan & J.S. Quick, 1993. An optimized fluorescence in situ hybridization procedure for detecting rye chromosomes in wheat. Genome 36: 701–705.

    PubMed  CAS  Google Scholar 

  • Orellana, J., 1985. Most of the homoeologous pairing at metaphase I in wheat-rye hybrids is not chiasmatic. Genetics 111: 917–931.

    PubMed  CAS  Google Scholar 

  • Petersen, G., B. Johansen & O. Seberg, 1996. PCR and sequencing from a single pollen grain. Plant Mol Biol 31: 189–191.

    Article  PubMed  CAS  Google Scholar 

  • Pfosser, M., A. Amon, J. Lafferty, E. Heberle-Bors & T. Lelley, 1995a. Gain or loss of single chromosomes in wheat-rye addition lines and in 6x triticale detected by flow cytometry. Plant Breed 114: 555–557.

    Article  Google Scholar 

  • Pfosser, M., A. Amon, T. Lelley & E. Heberle-Bors, 1995b. Evaluation of sensitivity of flow cytometry in detecting aneuploidy in wheat using disomic and ditelosomic wheat-rye addition lines. Cytometry 21: 387–393.

    Article  PubMed  CAS  Google Scholar 

  • Rabinovich, S.V., 1998. Importance of wheat-rye translocations for breeding modern cultivars of Triticum aestivum L. Euphytica 100: 323–340.

    Article  Google Scholar 

  • Rai, R.K., 1983. Strategies for exploiting cultivated rye (Secale cereale L.) in breeding winter wheat. Proc. 6th Int. Wheat Genet. Symp., Kyoto, Japan. pp. 181–186.

  • Rajaram, S., Ch.E. Mann, G. Ortiz-Ferrara & A. Mujeeb-Kazi, 1983. Adaptation, stability and high yield potential of certain 1B/1R CIMMYT wheats. Proc. 6th Int. Wheat Genet. Symp., Kyoto, Japan. pp. 613–621.

  • Rayburn, A.L. & B.F. Carver, 1988. Cytological identification of 1B/1R wheat-rye translocations in winter wheat breeding lines. Euphytica 38: 237–240.

    Article  Google Scholar 

  • Rayburn, A.L. & D.W. Mornhinweg, 1988. Inheritance of a 1BL/1RS wheat-rye translocated chromosome in wheat. Crop Sci 28: 709–711.

    Article  Google Scholar 

  • Ren, Z.L., T. Lelley & G. Robbelen, 1990. The use of monosomic rye addition lines for transferring rye chromatin into bread wheat. Plant Breed 105: 257–264.

    Article  Google Scholar 

  • Ren, S.X., R.A. McIntosh & Z.L. Lu, 1997. Genetic suppression of the cereal rye-derived gene Pm8 in wheat. Euphytica 93: 353–360.

    Article  Google Scholar 

  • Ren, S.X., R.A. McIntosh, P.J. Sharp & T.T. The, 1996. A storage-protein marker associated with the suppressor of Pm8 for powdery mildew resistance in wheat. Theor Appl Genet 93: 1054–1060.

    Article  Google Scholar 

  • Rimpau, J., D. Smith & R. Flavell, 1978. Sequence organisation analysis of the wheat and rye genomes by interspecies DNA/DNA hybridisation. J Mol Biol 123: 327–359.

    Article  PubMed  CAS  Google Scholar 

  • Rogers, W.J., J.M. Rickatson, E.J. Sayers & C.N. Law, 1990. Dosage effects of chromosomes of homoeologous groups 1 and 6 upon bread-making quality in hexaploid wheat. Theor Appl Genet 80: 281–287.

    Article  Google Scholar 

  • Rogowsky, P.M., M.E. Sorrells, K.W. Shepherd & P. Langridge, 1993. Characterisation of wheat-rye recombinants with RFLP and PCR probes. Theor Appl Genet 85: 1023–1028.

    Article  CAS  Google Scholar 

  • Saiki, R.K., D.H. Gelfland, S. Stoffel, S.J. Scharf, R. Higuchi, G.T. Horn, K.B. Mullis & H.A. Erlich, 1988. Primer-directed enzymatic amplification of DNA with thermostable DNA polymerase. Science 252: 1643–165.

    Google Scholar 

  • Sarma, N.P. & A.T. Natarajan, 1973. Identification of heterochromatic regions in the chromosomes of rye. Hereditas 74: 233–238.

    Article  Google Scholar 

  • Schlegel, R., 1997. Current list of wheats with rye introgressions of homoeologous group 1. 2nd update. Wheat Inf Serv 84: 64–69.

    Google Scholar 

  • Schlegel, R. & V. Korzun, 1997. About the origin of 1RS.1BL wheat-rye chromosome translocations from Germany. Plant Breed 116: 537–540.

    Article  Google Scholar 

  • Schlegel, R. & Meinel, 1994. A quantitative trait locus (QTL) on chromosome arm 1RS of rye and its effect on yield performance of hexaploid wheat. Cereal Res Commun 22: 7–13.

    Google Scholar 

  • Schlegel, R., G. Melz & V. Korzun, 1998. Genes, marker and linkage data of rye (Secale cereale L.): 5th updated inventory. Euphytica 101: 23–67.

    Article  CAS  Google Scholar 

  • Schlegel, R., G. Melz & D. Mettin, 1986. Rye cytology, cytogenetics and genetics-current status. Theor Appl Genet 72: 721–734.

    Article  Google Scholar 

  • Schram, A.W., 1988. Use and production of polyclonal and monoclonal antibodies. In: F. Franks (Ed), Characterization of proteins. Human Press, Clifton, NJ, USA. pp. 415–426.

    Google Scholar 

  • Schwarzacher, T., K. Anamthawat-Jonsson, G.E. Harrison, A.K.M.R. Islam, J.Z. Jia, I.P. King, A.R. Leitch, T.E. Miller, S.M. Reader, W.J. Rogers, M. Shi & J.S. Heslop-Harrison, 1992. Genomic in situ hybridization to identify alien chromosomes and chromosome segments in wheat. Theor Appl Genet 84: 778–786.

    Article  CAS  Google Scholar 

  • Seal, A.G. & M.D. Bennett, 1982. Preferential C-banding of wheat or rye chromosomes. Theor Appl Genet 63: 227–233.

    Article  Google Scholar 

  • Sebesta, E.E., E.A. Wood Jr., D.R. Porter, J.A. Webster & E.L. Smith, 1994. Registration of Amigo wheat germplasm resistant to greenbug. Crop Sci 34: 293.

    Article  Google Scholar 

  • Seo, Y.W., R.A. Graybosch, C.J. Peterson & D.R. Shelton, 1995. Assessment of enzyme-linked immunoassay of rye secalins as a tool in the prediction of 1RS wheat quality. Cereal Chem 72: 252–254.

    CAS  Google Scholar 

  • Sharma, A.K. & A. Sharma, 1980. Chromosome techniques: theory and practice, 3rd ed, Butterworths, London, UK.

    Google Scholar 

  • Sharp, P.J., S. Chao, S. Desai & M.D. Gale, 1989. The isolation, characterization and application in the Triticeae of a set of wheat RFLP probes identifying each homoeologous chromosome arm. Theor Appl Genet 78: 342–348.

    Article  Google Scholar 

  • Shepherd, K.W., 1973. Homoeology of wheat and alien chromosomes controlling endosperm protein phenotypes. Proc. 4th Int. Wheat Genet. Symp., Columbia, MO, USA. pp. 745–760.

  • Shewry, P.R., S. Parmar, N. Fulrath, D.D. Kasarda & T.E. Miller, 1986. Chromosomal locations of the structural genes for secalins in wild perennial rye (Secale montanum Guss.) and cultivated rye (S. cereale L.) determined by two-dimensional electrophoresis. Can J Genet Cytol 28: 76–83.

    CAS  Google Scholar 

  • Shimizu, Y., S. Nasuda & T.R. Endo, 1997. Detection of the Sec-1 locus of rye by a PCR-based method. Genes Genet Syst 72: 197–203.

    Article  PubMed  CAS  Google Scholar 

  • Singh, N.K. & K.W. Shepherd, 1984. Mapping of the genes controlling the high-molecular-weight glutenin subunits of rye on the long arm of chromosome 1R. Genet Res 44: 117–123.

    Article  CAS  Google Scholar 

  • Skerritt, J.H. & P.Y. Lew, 1990. Homologies between grain storage proteins of different cereal species. 1. Monoclonal antibody reaction with total protein extracts. J Cereal Sci 11: 103–121.

    Article  CAS  Google Scholar 

  • Southern, E.M., 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517.

    PubMed  CAS  Google Scholar 

  • Sreeramulu, G. & N.K. Singh, 1994. High Mr glutenin subunits of Indian wheat cultivars: association of subunits 5+10 with the 1BL/1RS translocation. J Cereal Sci 20: 217–225.

    Article  CAS  Google Scholar 

  • Talbert, L.E., N.K. Blake, P.W. Chee, T.K. Blake & G.M. Magyar, 1994. Evaluation of ‘sequence-tagged site’ PCR products as molecular markers in wheat. Theor Appl Genet 87: 789–794.

    Article  CAS  Google Scholar 

  • Tao, Y.Z., J.W. Snape & H. Hu, 1991. The cytological and genetic characterisation of doubled haploid lines derived from triticale × wheat hybrids. Theor Appl Genet 81: 369–375.

    Article  Google Scholar 

  • Tarkowski, Cz. & B. Apolinarska, 1992. The use of chromosome substitutions and translocations in the breeding of triticale, wheat and rye. Hereditas 116: 281–283.

    Google Scholar 

  • Vaccino, P. & E.V. Metakovsky, 1995. RFLP patterns of gliadin alleles in Triticum aestivum L.: implications for analysis of the organization and evolution of complex loci. Theor Appl Genet 90: 173–181.

    Article  CAS  Google Scholar 

  • Vahl, U. & G. Muller, 1998. Verification of T1AL.1RS, T1BL.1RS double translocation in wheat anther culture regenerates by glucose phosphate isomerase. In: T. Lelley (Ed), Current topics in plant cytogenetics related to plant improvement. WUV-Univ.-Verl., Austria. pp. 349–357.

    Google Scholar 

  • Vahl, U., G. Muller & T. Bohme, 1993. Electrophoretic protein analysis for the identification of doubled haploid 1A-1R, 1B-1R wheat-rye double translocation lines and for the assessment of their genetic stability. Theor Appl Genet 86: 547–556.

    Article  CAS  Google Scholar 

  • Van Campenhout, S., R. Aert & G. Volckaert, 1998. Othologous DNA sequence variation among 5S ribosomal RNA gene spacer sequences on homoeologous chromosomes 1B, 1D, and 1R of wheat and rye. Genome 41: 244–255.

    Article  PubMed  CAS  Google Scholar 

  • Van Campenhout, S, J. Vander Stappen, L. Sagi & G. Volckaert, 1995. Locus-specific primers For LMW glutenin genes on each of the group 1 chromosomes of wheat. Theor Appl Genet 91: 313–319.

    CAS  Google Scholar 

  • Vesterberg, O., 1993. A short history of electrophoretic methods. Electrophoresis 14: 1243–1249.

    Article  PubMed  CAS  Google Scholar 

  • Villareal, R.L, O. Banuelos & A. Mujeeb-Kazi, 1997. Agronomic performance of related durum wheat (Triticum turgidum L.) stocks possessing the chromosome substitution T1BL.1RS. Crop Sci 37: 1735–1740.

    Article  Google Scholar 

  • Villareal, R.L., S. Rajaram, A. Mujeeb-Kazi & E. Del Toro, 1991. The effect of chromosome 1B/1R translocation on the yield potential of certain spring wheats (Triticum aestivum L.). Plant Breed 106: 77–81.

    Article  Google Scholar 

  • Wang, M.L., M.D. Atkinson, C.N. Chinoy, K.M. Devos, R.L. Harcourt, C.J. Liu, W.J. Rogers & M.D. Gale, 1991. RFLP-based genetic map of rye (Secale cereale L.) chromosome 1R. Theor Appl Genet 82: 174–178.

    CAS  Google Scholar 

  • Wang, E., H. Xing, Y. Wen, W. Zhou, R. Wei & H. Han, 1998. Molecular and biochemical characterization of a non-Robertsonian wheat-rye chromosome translocation line. Crop Sci 38: 1076–1080.

    Article  CAS  Google Scholar 

  • Weegels, P.L., R.J. Hamer & J.D. Schofield, 1996. Critical Review: functional properties of wheat glutenin. J Cereal Sci 23: 1–18.

    Article  CAS  Google Scholar 

  • William, M.D.H.M. & A. Mujeeb-Kazi, 1993. Rapid detection of 1B, 1BL/1RS heterozygotes in the development of homozygous 1BL/1RS translocation stocks of Triticum turgidum (2n = 2x = 28). Genome 36: 1088–1091.

    CAS  PubMed  Google Scholar 

  • William, M.D.H.M., O. Riera-Lizarazu & A. Mujeeb-Kazi, 1992. A combination of protein electrophoretic techniques for the detection of 1B, 1B/1R heterozygotes in Triticum aestivum L. J Genet Breed 46: 137–142.

    CAS  Google Scholar 

  • Williams, C.E. & P.C. Ronald, 1994. PCR template-DNA isolated quickly from monocot and dicot leaves without tissue homogenization. Nucleic Acids Res 10: 1917–1918.

    Google Scholar 

  • Zeller, F.J., 1973. 1B/1R wheat-rye chromosome substitutions and translocations. 4th Int. Wheat Genet. Symp., Columbia, MO, USA. pp. 209–221.

  • Zeller, F.J. & S.L.K. Hsam, 1983. Broadening the genetic variability of cultivated wheat by utilizing rye chromatin. Proc. 6th Int. Wheat Genet. Symp., Kyoto, Japan. pp. 161–173.

  • Zeller, F.J. & S.L.K. Hsam, 1996. Chromosomal location of a gene suppressing powdery mildew resistance genes Pm8 and Pm17 in common wheat (Triticum aestivum L. em Thell.). Theor Appl Genet 93: 38–40.

    Article  CAS  Google Scholar 

  • Zhang, W., S.W. Zhang, R.X. Wei & H. Hu, 1993. Identification of rye chromosome segments in in wheat-rye pollen-derived lines by in situ hybridization and giemsa C-banding. 8th Int. Wheat Genet. Symp., Beijing, China. pp. 549–551.

  • Zhen, Z. & D. Mares, 1992. A simple extraction and one-step SDSPAGE system for separating HMW and LMW glutenin subunits of wheat and high molecular weight proteins of rye. J Cereal Sci 15: 63–78.

    Google Scholar 

  • Zhu, Z., J. Chao, H. Yu & A.S. Waggoner, 1994. Directly labeled DNA probes using fluorescent nucleotides with different length linkers. Nucleic Acids Res 22: 3418–3422.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Plant Sciences Department, North Dakota State University, Fargo, ND, 58105, .S.A.

    William A. Berzonsky

  2. Botany Department, University of Western Australia, Nedlands, WA, 6907, Australia

    Michael G. Francki

Authors
  1. William A. Berzonsky
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Michael G. Francki
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Berzonsky, W.A., Francki, M.G. Biochemical, molecular, and cytogenetic technologies for characterizing 1RS in wheat: A review. Euphytica 108, 1–19 (1999). https://doi.org/10.1023/A:1003638131743

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1003638131743

Search

Navigation