Abstract
Fatty acid analysis was used for determining the extent of the development of the external mycelium of AM fungi (mixed inoculum from a sand dune) growing from roots of Festuca rubra and Plantago lanceolata in calcareous dune sand. The plants were raised in chambers specially designed to permit the growth of AM mycelium in root-free compartments. In two separate experiments, growth of external mycelium in the root-free compartments was detected and the amount of mycelium was estimated using the indicator of AM fungal biomass, phospholipid fatty acid (PLFA) 16:1ω5. The results showed that the PLFA 16:1ω5 was suitable for estimating the mycelium emerging from the mixed inoculum obtained from the field roots of F. rubra and P. lanceolata.
The PLFA 16:1ω5 showed external mycelium to become established in the root-free compartments within a period of 3 weeks and the amount of mycelium to continue to increase at 6 and 9 weeks. Increases in neutral lipid fatty acid (NLFA) 16:1ω5 (indicator of storage lipids) over time were inconsistent between the two experiments, but appeared to follow patterns of sporulation in each experiment.
In both experiments, the root-free compartment was colonised by saprophytic fungi to a greater extent in the case of non-mycorrhizal than of AM treatment, as indicated by an increase in PLFA 18:2ω6,9 (indicator of saprophytic fungi). The absence of an increase in the case of AM treatment indicates that AM fungal mycelium can negatively affect the growth of saprophytic fungi in this soil type. This result was, however, only weakly supported by measurements of ergosterol content. The analysis of bacteria specific PLFAs showed that bacterial biomass was not affected by the AM mycelium.
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References
Abbott L K and Robson A D 1985 Formation of external hyphae in soil by four species of vesicular-arbuscular mycorrhizal fungi. New Phytol. 99, 245–255.
Bååth E, Frostegård Å and Fritze H 1992 Soil bacterial biomass, activity, phospholipid fatty acid pattern, and pH tolerance in an area polluted with alkaline dust deposition. Appl. Environ. Microbiol. 58, 4026–4031.
Beilby J P and Kidby D K 1980 Sterol composition of ungerminated and germinated spores of the vesicular-arbuscular mycorrhizal fungus, Glomus caledonius. Lipids 15, 375–378.
Cooper K M and Lösel D M 1978 Lipid physiology of vesicular-arbuscular mycorrhiza I. Composition of lipids in roots of onion, clover and ryegrass infected with Glomus mosseae. New Phytol. 80, 143–151.
Federle T W 1986 Microbial distribution in soil — new techniques. In Perspectives in Microbial Ecology. Eds. F Megusar and M Gantar. pp 493–498. Slovene Society for Microbiology, Ljubljana, Yugoslavia.
Francis R and Read D J 1994 The contributions of mycorrhizal fungi to the determination of plant community structure. Plant Soil 159, 11–25.
Francis R and Read D J 1995 Mutualism and antagonism in the mycorrhizal symbiosis, with special reference to impacts on plant community structure. Can. J. Bot. (Suppl. 1), S1301-S1309.
Frey B, Vilarino A, Schüepp H and Arines J 1994 Chitin and ergosterol content of extraradical and intraradical mycelium of the vesicular-arbuscular mycorrhizal fungus Glomus intraradices. Soil Biol. Biochem. 26, 711–717.
Frostegård Å and Bååth E 1996 The use of phospholipid fatty acid analysis to estimate bacterial and fungal biomass in soil. Biol. Fertil. Soils 22, 59–65.
Frostegård Å, Bååth E and Tunlid A 1993 Shifts in the structure of soil microbial communities in limed forests as revealed by phospholipid fatty acid analysis. Soil Biol. Biochem. 25, 723–730.
Gerdemann J W and Nicolson T H 1963 Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting. Trans. Brit. Mycol. Soc. 46, 235–244.
Graham J H, Hodge N C and Morton J B 1995 Fatty acid methyl ester profiles for characterization of Glomalean fungi and their endomycorrhizae. Appl. Environ. Microbiol. 61, 58–64.
Harinikumar K M and Bagyaraj D J 1995 Spread of vesicular arbuscular mycorrhizal fungal hyphae in soil. Microbiol. Res. 150, 77–80.
Jakobsen I, Abbott L K and Robson A D 1992 External hyphae of vesicular arbuscular mycorrhizal fungi associated with Trifolium subterranium. I. Spread of hyphae and phosphorus inflow into roots. New Phytol. 120, 371–380.
Jensen V 1962 Studies on the microflora of Danish beech forest soils. I. The dilution plate count technique for the enumeration of bacteria and fungi in soil. Zbl. Bakt. Abt. II, Bd. 116, 13–32.
Johansen A, Finlay R D and Olsson P A 1996 Nitrogen metabolism of external hyphae of the arbuscular mycorrhizal fungus Glomus intraradices. New Phytol. 133, 705–712.
Lechevalier H and Lechevalier M P 1988 Chemotaxonomic use of lipids — an overview. In Microbial Lipids, Vol. 1. Eds. C Ratledge and S G Wilkinson. pp 869–902. Academic Press, San Diego, USA.
Morton J N and Benny G L 1990 Revised classification of arbuscular mycorrhizal fungi (Zygomycetes): A new order, Glomales, two new suborders, Glomineae and Gigasporineae, and two new families, Acaulosoraceae, and Gigasporaceae, with an emendation of Glomaceae. Mycotaxon 37, 471–491.
Müller M M, Kantola R and Kitunen V 1994 Combining sterol and fatty acid profiles for the characterization of fungi. Mycol. Res. 98, 593–603.
Nagy S, Nordby H E and Nemec S 1980 Composition of lipids in roots of six citrus cultivars infected with the vesicular arbuscular mycorrhizal fungus Glomus mosseae. New Phytol. 85, 377–384.
Neidhardt F C, Ingraham J L and Schaechter M 1990 Physiology of the bacterial cell. A molecular approach. Sinauer Associates, Sunderland, Massachusetts, USA.
Nylund J-E and Wallander H 1992 Ergosterol analysis as a means of quantifying mycorrhizal biomass. In Methods in Microbiology: Techniques for the Study of Mycorrhiza, Vol. 24. Eds. J R Norris, D J Read and A K Varma. pp 77–88. Academic Press, London, UK.
Olsson P A, Bååth E, Jakobsen I and Söderström B 1995 The use of phospholipid and neutral lipid fatty acids to estimate biomass of arbuscular mycorrhizal fungi. Mycol. Res. 99, 623–629.
Olsson P A, Bååth E and Jakobsen I 1997 Phosphorus effects on the mycelium and storage structures of an arbuscular mycorrhizal fungus as studied in the soil and roots by analysis of fatty acid signatures. Appl. Environ. Microbiol. 63, 3531–3538.
Olsson P A, Bååth E, Jakobsen I and Söderström B 1996 Soil bacteria respond to presence of roots but not to mycelium of arbuscular mycorrhizal fungi. Soil Biol. Biochem. 28, 463–470.
Read D J 1984 The structure and function of the vegetative myceliumof mycorrhizal roots. In The Ecology and Physiology of the Fungal Mycelium. Eds. D H Jennings and A D M Rayner. pp 215–239. Cambridge University Press, UK.
Sylvia D M 1992 Quantification of external hyphae of vesicular-arbuscular mycorrhizal fungi. In Methods in Microbiology: Techniques for the Study of Mycorrhiza, Vol. 24. Eds. J R Norris, D J Read and A K Varma. pp 53–65. Academic Press, London, UK.
Tunlid A, Hoitink H A C, Low C and White D C 1989. Characterization of bacteria that supress Rhizoctonia dampingoff in bark compost media by analysis of fatty acid biomarkers. Appl. Environ. Microbiol. 55, 1368–1374.
Tunlid A and White D C 1992 Biochemical analysis of biomass, community structure, nutritional status, and metabolic activity of microbial communities in soil. In Soil Biochemistry, Vol. 7. Eds. G Stotzky and J-M Bollag. pp 229–262. Dekker, New York.
Walker R W 1969 Cis-11-hexadecanoic acid from Cytophaga hutchinsonii lipids. Lipids 4, 15–18.
Wellburn A R, Robinson D C, Thomson A and Leith I D 1994 Influence of episodes of summer O3 and Δ5 and Δ9 fatty acids in automnal lipids of Norway spruce [Picea abies (L.) Karst]. New Phytol. 127, 355–361.
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Olsson, P., Francis, R., Read, D. et al. Growth of arbuscular mycorrhizal mycelium in calcareous dune sand and its interaction with other soil microorganisms as estimated by measurement of specific fatty acids. Plant and Soil 201, 9–16 (1998). https://doi.org/10.1023/A:1004379404220
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DOI: https://doi.org/10.1023/A:1004379404220