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Site of Interaction of Copper on Bacillus Polymyxa

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Abstract

Copper uptake potential of different bacterial strains isolatedfrom contaminated soil was compared. The bacterial strainidentified as Bacillus polymyxa showed maximum copperuptake capacity. An attempt was made to elucidate the site ofinteraction of Cu(II) with B. polymyxa using electronparamagnetic resonance (EPR) studies. The EPR spectra showedthat accumulated copper formed complexes with oxygen andnitrogen which may be the carboxyl groups of cell-wallpeptidoglycan and nitrogen atoms of aminosugars or structuralproteins. The absence of super hyperfine splitting in theg ⊥ region of EPR spectra of treated B. polymyxa cellsafter Cu(II) uptake indicated that copper could be coordinatedwith the oxygen atoms from carboxyl groups of peptidoglycan inthe cell walls.

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Authors and Affiliations

  1. Dept. of Civil Engineering, Indian Institute of Technology, Kharagpur, India, 721 302

    Ligy Philip

  2. Dept. of Chemistry, Indian Institute of Technology, Kanpur, India

    Leela Iyengar

  3. Dept. of Civil Engineering, University of West Indies, St. Augustine, Trinidad, WI

    C. Venkobachar

Authors
  1. Ligy Philip
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  2. Leela Iyengar
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  3. C. Venkobachar
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Correspondence to Ligy Philip.

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Philip, L., Iyengar, L. & Venkobachar, C. Site of Interaction of Copper on Bacillus Polymyxa. Water, Air, & Soil Pollution 119, 11–21 (2000). https://doi.org/10.1023/A:1005123624184

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