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Hydroxybenzotriazole increases the range of textile dyes decolorized by immobilized laccase

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Abstract

Laccase from Coriolopsis gallica UAMH8260 was immobilized on activated agarose and tested for repeated decolorization of industrial dyes. Immobilized enzyme retained 85% of the initial activity after 10 cycles, and 70% after 3 months of intermittant use in the decolorization of Reactive Blue 198 dye. Free laccase decolorized 13 of 38 industrial dyes tested but, in the presence of 1 mM 1-hydroxybenzotriazole as a free radical mediator, the enzyme decolorized 26 of the 38 dyes increasing both the range and rate of decolorization. Immobilized laccase showed a higher thermal stability at 70 °C than free enzyme but no increased resistance to organic solvents.

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Authors and Affiliations

  1. Instituto de Biotecnologia-UNAM, Apartado Postal 510-3, Cuernavaca, Morelos, 62250, Mexico

    Pilar Reyes & Rafael Vazquez-Duhalt

  2. Department of Biological Sciences, University of Alberta, Edmonton, Canada, T6G 2E9

    Michael A. Pickard

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  1. Pilar Reyes
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  2. Michael A. Pickard
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  3. Rafael Vazquez-Duhalt
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Reyes, P., Pickard, M.A. & Vazquez-Duhalt, R. Hydroxybenzotriazole increases the range of textile dyes decolorized by immobilized laccase. Biotechnology Letters 21, 875–880 (1999). https://doi.org/10.1023/A:1005502906890

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