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Enhanced forskolin production in genetically transformed cultures of Coleus forskohlii by casein hydrolysate and studies on growth and organisation

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Abstract

Casein hydrolysate at 2.0 g l−1 significantly enhanced forskolin content (2.3 mg g−1 cell dry wt) in a rhizogenic tumourous line, GCO-RCH-2 of Coleus forskohlii. In rooty teratoma line, RC-ST-2/4, forskolin content enhanced to 1.7 mg g−1 cell dry wt in presence of 2.5 g l−1 casein hydrolysate. Unlike untransformed calli and rhizogenic/root cultures, all the forskolin yielding transformed cultures of C. forskohlii have been maintained for over 5 years.

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Authors and Affiliations

  1. Centre of Advanced Study (Cell and Chromosome Research), Department of Botany, University of Calcutta, 35, B.C. Road, Calcutta, 700 019, India

    Swapna Mukherjee, Biswajit Ghosh & Sumita Jha

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  1. Swapna Mukherjee
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  2. Biswajit Ghosh
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  3. Sumita Jha
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Mukherjee, S., Ghosh, B. & Jha, S. Enhanced forskolin production in genetically transformed cultures of Coleus forskohlii by casein hydrolysate and studies on growth and organisation. Biotechnology Letters 22, 133–136 (2000). https://doi.org/10.1023/A:1005618307074

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  • DOI: https://doi.org/10.1023/A:1005618307074

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