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Formation of lipolytic enzymes by Brevibacterium linens

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Abstract

When Brevibacterium linens ATCC 9172 was grown in shake flasks, it produced a cell-associated lipase with a specific activity of 152 to 188 U g−1 cells depending on the composition of the growth medium. There was no growth in media containing tributyrine as the sole carbon source. The cell-associated lipase had maximum activity at pH 8.0 and 37 °C and was strongly inhibited by 3,4-dichloroisocoumarin, an inhibitor specific for serine esterases. Cell-associated activity was released from the cells by treatment with lysozyme. The kinetics of lipase formation was closely related to the amount of biomass formed during growth.

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References

  • Brandl E, Petutschnig K (1972) —Orientierende Untersuchungen über die proteolytische und lipolytische Aktivität einiger Brevibacterium linens Stämme verschiedener Herkunft. österr. Milchwirtsch., Wiss. Beil. 27: 17-26.

    Google Scholar 

  • Buchinger W (1999) Optimierung der Bedingungen für das Wachstum und die Bildung proteolytischer Enzyme von Brevibacterium linens. Thesis, Vienna University of Technology, Austria.

    Google Scholar 

  • Clancy M, O'Sullivan M (1993) Partial purification and characterization of a proteinase from Brevibacterium linens. Ir. J. Agric. Food Res. 32: 185-194.

    Google Scholar 

  • Hayashi K, Cliffe AJ, Law BA (1990) Purification and preliminary characterization of five serine proteinases produced by Brevibacterium linens. Int. J. Food Sci. Technol. 25: 180-187.

    Google Scholar 

  • Hosono A (1968) Production of volatile fatty acids by Brevibacterium linens. II. Formation of volatile fatty acids from some carbohydrates and butter fat. Jap. J. Zootech. Sci. 39: 212-219.

    Google Scholar 

  • Jollivet N, Bezenger MC, Vayssier Y, Belin JM (1992) Production of volatile compounds in liquid cultures by six strains of coryneform bacteria. Appl. Microbiol. Biotechnol. 36: 790-794.

    Google Scholar 

  • Papon M, Talon R (1989) Cell location and partial characterization of Bronchotrix thermosphacta and Lactobacillus curvatus lipases. J. Appl. Bacteriol. 66: 235-242.

    Google Scholar 

  • Pereira-Meirelles FV, Rocha-Leao MHM, Sant'Anna Jr GL (2000) Lipase location in Yarrowia lipolytica cells. Biotechnol. Lett. 22: 71-75.

    Google Scholar 

  • Rattray FP, Bockelmann W, Fox PF (1995) Purification and characterization of an extracellular proteinase from Brevibacterium linens ATCC 9174. Appl. Environ. Microbiol. 61: 3454-3456.

    Google Scholar 

  • San Clemente CL, Vadehra DV (1967) Instrumental assay of microbial lipase at constant pH. Appl. Microbiol. 15: 110-113.

    PubMed  Google Scholar 

  • Sørhaug T, Ordal ZJ (1974) Cell-bound lipase and esterase of Brevibacterium linens. Appl. Microbiol. 25: 607-608.

    Google Scholar 

  • Strauss S, Kopetcky A, Zemanovic J, Hampel WA (1994) Production of proteases from Brevibacterium linens. Biocatalysis 10: 317-323.

    Google Scholar 

  • Taipa MA, Aires-Barros MR, Cabral JMS (1992) Purification of lipases. J. Biotechnol. 26: 111-142.

    PubMed  Google Scholar 

  • Wolin EA, Wolin MJ, Wolfe RS (1963) Formation of methane by bacterial extracts. J. Biol. Chem. 238: 2882-2886.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of Biochemical Technology and Microbiology, Vienna University of Technology, Getreidemarkt 9/172, A-1060, Vienna, Austria

    Bernard F. Adamitsch & Werner A. Hampel

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  1. Bernard F. Adamitsch
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  2. Werner A. Hampel
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Adamitsch, B.F., Hampel, W.A. Formation of lipolytic enzymes by Brevibacterium linens. Biotechnology Letters 22, 1643–1646 (2000). https://doi.org/10.1023/A:1005633828125

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