Abstract
We investigated the expression and accumulation pattern of lipoxygenaseisoforms throughout the maize plant life. Two forms of lipoxygenase L1and L2 have been identified as acidic proteins of 100 kDa (pI 6.4) and90 kDa (pI 5.5-5.7) which accumulate in dry embryos and in variousorgans of maize seedlings. In young embryos, only the L2 form wasdetected and accumulation of L2 mRNA decreased during embryodevelopment. Identification of lipoxygenases from in vivo and in vitro synthesized proteins indicates that similar levels of both L1and L2 forms accumulated during treatment with abscisic acid, (ABA)gibberellic acid (GA3) and jasmonic acid (JA). However,differences in the activity of both enzymes were detected. By using anantiserum directed against purified L2 we isolated and characterized apartial cDNA clone of maize embryos encoding a lipoxygenase. The deducedamino acid sequence of L2 cDNA shares 78% identity with the rice L2protein, and 51-56% identity with lipoxygenases from thedicotyledonous plants soybean and Arabidopsis/. DNA blotanalysis indicated that maize contains a family of lipoxygenase geneswhich are presently being characterized.
Similar content being viewed by others
References
Abián J, Gelpi E, Pagès M: Effect of abscisic acid on the linoleic acid metabolism in developing maize embryos. Plant Physiol 95: 1277–1283 (1991).
Altschuler M, Grayburn WS, Collins GB, Hildebrand DF: Developmental expression of lipoxygenases in soybean. Plant Sci 63: 151–158 (1989).
Anderson JM: Membrane-derived fatty acids as precursors to second messengers. In: Boss WF, Morr DJ (eds) Second Messengers in Plant Growth and Development, pp. 181–212. Alan Liss, New York (1989).
Bell E, Mullet JE: Lipoxygenase gene expression is modulated in plants by water deficit, wounding, and methyl jasmonate. Mol Gen Genet 230: 456–462 (1991).
Bell E, Mullet JE: Characterization of an Arabidopsis thaliana lipoxygenase gene responsive to methyl jasmonate and wounding. Plant Physiol 103: 1133–1137 (1993).
Creelman RA, Bell E, Mullet J: Involvement of a lipoxygenaselike enzyme in abscisic acid biosynthesis. Plant Physiol 99: 1258–1260 (1992).
Creelman RA, Tierney ML, Mullet J: Jasmonic acid/methyl jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression. Proc Natl Acad Sci USA 89: 4938–4941 (1992).
Croft KPC, Jutter F, Slusarenko AJ: Volatile products of the lipoxygenase pathway evolved from Phaseolus vulgaris (L.) leaves inoculated with Pseudomonas syringae pv. phaseolicola. Plant Physiol 101: 13–24 (1993).
Dellaporta SL, Wood J, Hicks JB: A plant DNA minipreparation: Version II. Plant Mol Biol Rep 1: 19–21 (1983).
Eiben HG, Slusarenko AJ: Complex spatial and temporal expression of lipoxygenase genes during Phaseolus vulgaris (L.) development. Plant J 5: 123–135 (1994).
Farmer EE, Ryan CA: Octadecanoid precursors of jasmonic acid activate the synthesis of wound-inducible proteinase inhibitors. Plant Cell 4: 129–134 (1992).
Firn RD, Friend J: Enzymatic production of the plant growth inhibitor, xanthoxin. Planta 103: 263–266 (1972).
Grayburn WS, Schneider GR, Hamilton-Kemp TR, Bookjans G, Ali K, Hildebrand DF: Soybean leaves contain multiple lipoxygenases. Plant Physiol. 95: 1214–1218 (1991).
Hildebrand DF, Hamilton-Kemp TR, Legg CS, Bookjans G: Plant lipoxygenases: occurrences, properties and possible functions. Curr Top Plant Biochem Physiol 7: 201–219 (1988).
Kock E, Meier BM, Eiben H-G, Slusarenko A: A lipoxygenase from leaves of tomato (Lycopersicum esculentum Mill.) is induced in response to plant pathogenic Pseudomonas. Plant Physiol 99: 571–576 (1992).
Logeman J, Schell J, Willmitzer L: Improved method for the isolation of RNA from plant tissues. Anal Biochem 163: 16–20 (1987).
Melan MA, Dong X, Endara ME, Davis KR, Ausubel FM, Peterman TK: An Arabidopsis thaliana lipoxygenase gene can be induced by pathogens, abscisic acid and methyl jasmonate. Plant Physiol 101: 441–450 (1993).
Nguyen T, Falgueyret J-P, Abremovitz M, Riendeau D: Evaluation of the role of conserved his and met residues among lipoxygenases by site-directed mutagenesis of recombinant human 5-lipoxygenase. J Biol Chem 266: 22057–22062 (1991).
Ohta H, Shida K, Peng Y-L, Furusawa I, Shishiyama J, Aibara S, Morita Y: A lipoxygenase pathway is activated in rice after infection with the rice blast fungus Magnaporthe grisea. Plant Physiol 97: 94–98 (1991).
Ohta H, Shirano Y, Tanaka K, Morita Y, Shibata D: cDNA cloning of rice lipoxygenase L-2 and characterization using an active enzyme expressed from the cDNA in Escherichia coli. Eur J Biochem 206: 331–336 (1992).
Park TK, Polacco JC: Distinct lipoxygenase species appear in the hypocotyl/radicle of germinating soybean. Plant Physiol 90: 285–290 (1989).
Parthier B: Jasmonates, new regulators of plant growth and development: many facts and few hypotheses on their actions. Bot Acta 104: 446–454 (1991).
Poca E, Rabinovitch-Chable H, Cook-Moreau J, Pages M, Rigaud M: Lipoxygenases from Zea mays L. Purification and physicochemical characteristics. Biochim Biophys Acta 1045: 107–114 (1990).
Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
Samuelson B, Dahlen SE, Lindgren J, Rouzer CA, Serhan CN: Leukotrienes and lipoxins: structures, biosynthesis, and biological effects. Science 237: 1171–1176 (1987).
Schewe T, Rapaport SM, Kuhn H: Enzymology and physiology of reticulocyte lipoxygenase: a comparison with other lipoxygenases. Adv Enzymol Mol Biol 58: 191–272 (1986).
Shibata D, Steczko J, Dixon JE, Andrews PC, Hermodson M, Axelrod B: Primary structure of soybean lipoxygenase L-2*. J Biol Chem 263: 6816–6821 (1988).
Shibata D, Steczko J, Dixon JE, Hermodson M, Yazdanparast R, Axelrod B: Primary structure of soybean lipoxygenase-1. J Biol Chem 262: 10080–10085 (1987).
Siedow JN: Plant lipoxygenases: structure and function. Annu Rev Plant Physiol Plant Mol Biol 42: 145–188 (1991).
Singh NK, LaRosa PC, Handa AD, Hasagava PM, Bressan RA: Hormonal regulation of protein synthesis associated with salt tolerance in plant cells. Proc Natl Acad Sci USA 84: 739–743 (1987).
Taylor HF, Burden RS: Preparation and metabolism of 2-[14C]-cis, trans-xanthin. J Exp Bot 24: 873–880 (1973).
Tranberger TJ, Franceschi VR, Hildebrand DF, Grimes HD: The soybean 94-kd vegetative storage protein is a lipoxygenase that is localized in paravenial mesophyll cell vacuoles. Plant Cell 3: 973–987 (1991).
Vick BA, Zimmerman DC: The lipoxygenase pathway. In: Stum PK, Mudd JB, Nes WD The Metabolism, Structure and Function of Plant Lipids, pp. 383–390. Plenum Press, New York (1987).
Yenofsky RL, Fine M, Liu C: Isolation and characterization of a soybean (Glycine max) lipoxygenase-3 gene. Mol Gen Genet 211: 215–222 (1988).
Zeevaart JAD, Creelman RA: Metabolism and physiology of abscisic acid. Annu Rev Plant Physiol Plant Mol Biol 39: 439–473 (1988).
Zhang Y, Radmark O, Samuelsson B: Mutagenesis of some conserved residues in human 5-lipoxygenase: effects on enzyme activity. Proc Natl Acad Sci USA 89: 485–489 (1992).
Zimmerman DC, Coudron CA: Identification of traumatin, a wound hormone, as 12-oxo-trans-10-dodecanoic acid. Plant Physiol 63: 536–541 (1979).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jensen, A.B., Poca, E., Rigaud, M. et al. Molecular characterization of L2 lipoxygenase from maize embryos. Plant Mol Biol 33, 605–614 (1997). https://doi.org/10.1023/A:1005742719019
Issue Date:
DOI: https://doi.org/10.1023/A:1005742719019