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Changes in the photosynthetic pigments in bean leaves during the first photoperiod of greening and the subsequent dark-phase. Comparison between old (10-d-old) leaves and young (2-d-old) leaves

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Abstract

Chlorophyll and carotenoid variations of 2-d-old and 10-d-old bean leaves (Phaseolus vulgaris var Red Kidney) were analyzed by HPLC during the first photoperiod of greening (16 h light + 8 h dark). The HPLC method used is suitable for the separation of cis- and trans-carotenoid isomers, Pchlide a and Chlide a as well as their esters. The main results are (1) before illumination the composition of the carotenoid pool is similar at the two developmental stages; (2) non-illuminated 2-d-old leaves are devoid of Pchlide a ester; (3) chlorophyll and carotenoid accumulation in 2-d-old leaves presented a lag phase twice longer than observed in 10-d-old ones; (4) Chlide a seems directly esterified to Chl a in 2-d-old leaves whereas esterification requires four steps in 10-d-old leaves and, (5) the kinetics of Chl and carotenoid accumulation are different at the two investigated developmental stages.

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Authors and Affiliations

  1. Laboratory of Biomembranes, University of South Bohemia, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic

    Benoît Schoefs

  2. National Institute for Marine Sciences and Techniques, Conservatoire National des Arts et Métiers, BP 324, 50103, Cherbourg Cedex, France

    Martine Bertrand

  3. Laboratory of Plant Cytophysiology and Phycology (SN2), University of Lille 1, 59655, Villeneuve d'Ascq, France

    Yves Lemoine

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  1. Benoît Schoefs
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  2. Martine Bertrand
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  3. Yves Lemoine
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Schoefs, B., Bertrand, M. & Lemoine, Y. Changes in the photosynthetic pigments in bean leaves during the first photoperiod of greening and the subsequent dark-phase. Comparison between old (10-d-old) leaves and young (2-d-old) leaves. Photosynthesis Research 57, 203–213 (1998). https://doi.org/10.1023/A:1006000208160

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