Abstract
The effect of light quality on somatic embryogenesis in quince BA 29 was investigated. 2,4-D induced leaves were exposed for 25 days to the following light quality treatments: dark, far-red, far-red+blue, far-red+red, blue, white, red+blue, red. After a further 20 days of white light exposure, somatic embryo production was recorded. Somatic embryogenesis was highest in cultures subjected to red light treatment, and decreased progressively with the transition to red+blue and to white. Overall, embryogenic competence showed a correlation with photoequilibrium. Phytochrome appeared to be inductive although this effect was adversely influenced by the blue absorbing photoreceptor, in particular at low photoequilibrium. Independently of light treatments applied, somatic embryos frequently showed severe morphological abnormalities. Conversion of somatic embryos to plantlets was not observed.
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D'Onofrio, C., Morini, S. & Bellocchi, G. Effect of light quality on somatic embryogenesis of quince leaves. Plant Cell, Tissue and Organ Culture 53, 91–98 (1998). https://doi.org/10.1023/A:1006059615088
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DOI: https://doi.org/10.1023/A:1006059615088