Abstract
An efficient protocol was developed for in vitro clonal propagation of Plumbago zeylanica Linn. through nodal culture. Multiple shoots were induced from nodal explants of P. zeylanica on Murashige and Skoog's (1962) medium supplemented with 0.5 mg L−1 to 1.0 mg.L−1 6-benzyladenine and 3% (w/v) sucrose. Inclusion of IAA (0.01 mg L−1) in the culture medium improved the frequency of production of multiple shoots. Rooting was readily achieved upon transferring the shoots onto half-strength MS medium supplemented with 0.25 mg L−1 IBA and 2% (w/v) sucrose. Micropropagated plantlets were hardened in the greenhouse and successfully established in soil.
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References
Anonymous (1989) The Wealth of India: A Dictionary of Indian Raw Materials and Industrial Products, Vol. II, CSIR. India: New Delhi, pp 163–164
Barna KS and Wakhlu AK (1983) Axillary shoot induction and plant regeneration in Plantago ovata Forssk. Plant Cell Tiss and Org Cult 15: 169–173
Chaturvedi HC, Misra P and Jain M (1984) Proliferation of shoot-tips and clonal multiplication of Costus speciosus in long-term culture. Plant Sci Lett 35: 67–71
George EF and Sherrington PD (1984) Plant Propagation by Tissue Culture. Eversley, England: Exegetics Ltd
Hu CY and Wang PJ (1983) Techniques for propagation and breeding. In: Evans DA, Sharp WR, Ammirato PV and Yamada V (eds) Handbook of Plant Cell Culture, Vol. 1. New York: MacMillan Inc., pp 177–277
Jayaraman, KS (1987) India seeks scientific basis of traditional remedies. Nature (London) 326: 323
Krishnaswamy M and Purushothaman KK (1980) Plumbagin, a study of its anticancer, antibacterial and antifungal properties. Ind J Expt Biol 18: 876–877
Lal N, Ahuja PS, Kukreja AK and Panday B (1988) Clonal propagation of Picrorhiza kurroa Royle ex Benth by shoot-tip culture. Plant Cell Rep 7: 202–205
Marascuilo LA and McSweeney M (1977) Post-Hoc Multiple Comparisons in sample preparations for test of homogenesity. In: McSweeney M and Marascuilo LA (eds) Non-parametric and Distribution Free Methods the Social Sciences. CA, USA: Books/Cole Publ, pp 141–147
Mathew MK and Hariharan M (1990) In vitro multiple shoot regeneration in Syzygium aromaticum. Ann Bot 65: 277–279
Modi J (1961) Text Book of Medicinal Jurisprudence and Toxicology. Bombay, India: Pripati Pvt. Ltd., pp 595–596
Murashige T and Skoog, F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Rajeevan MS and Pandey RM (1986) Lateral bud culture of Papaya (Carica papaya L.) for clonal propagation. Plant Cell Tiss and Org Cult 6: 181–188
Satheesh Kumar K and Bhavanandan KV (1988) Micropropagation of Plumbago rosea Linn. Plant Cell, Tissue and Organ Culture 15: 275–278
Saxena C, Palai SK, Samantaray S, Rout GR and Das P (1997) Plant regeneration from callus cultures of Psoralea corylifolia Linn. Plant Growth Regul 22: 13–17
Saxena C, Rout GR and Das P (1998) Micropropagation of Psoralea corylifolia. J Medicinal and Aromatic Plant Sci 20: 15–18
Vieitez AM and Vieitez ML (1983) Castanea sativa plantlets, proliferated from axillary buds cultured in vitro. Scientia Hortic 18: 343–351
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Rout, G., Saxena, C., Das, P. et al. Rapid clonal propagation of Plumbago zeylanica Linn.. Plant Growth Regulation 28, 1–4 (1999). https://doi.org/10.1023/A:1006146713143
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DOI: https://doi.org/10.1023/A:1006146713143