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Effect of sugars, amino acids, and culture technique on maturation of somatic embryos of Pinus strobus on medium with two gellan gum concentrations

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Abstract

Maturation of five embryogenic lines of Pinus strobus L. was tested on media with various sugars and sources of organic nitrogen, and solidified with two gellan gum concentrations (0.6 and 1.0%). Mature somatic embryo production was more abundant at 1.0% gellan gum than at 0.6%. Complex combinations of amino acids had little effect on mature embryo production of most tested embryogenic lines. Increasing glutamine concentration of the maturation medium from 1.7 to 7.3 g l−1 was beneficial to one embryogenic line. Increasing sucrose concentration or substituting part of the sucrose with mannitol or sorbitol had variable effects on somatic embryo maturation depending on the embryogenic line. A medium with 88 mM sucrose plus 175 mM sorbitol solidified with 1.0% gellan gum produced high numbers of somatic embryos in four out of five embryogenic lines tested.

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Author notes

  1. Élisabeth Garin

    Present address: , Centre de recherche en biologie forestière, Pavillon C.-E. Marchand, Université Laval, Sainte-Foy, Québec, Canada, G1K 7P4

Authors and Affiliations

  1. Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, P.O. Box 3800, 1055 du P.E.P.S., Sainte-Foy, Québec, Canada, G1V 4C7

    Élisabeth Garin, Michèle Bernier-Cardou, Nathalie Isabel, Krystyna Klimaszewska, Krystyna Klimaszewska & Ariane Plourde

Authors
  1. Élisabeth Garin
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  2. Michèle Bernier-Cardou
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  3. Nathalie Isabel
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  4. Krystyna Klimaszewska
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  5. Ariane Plourde
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Correspondence to Krystyna Klimaszewska.

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Garin, É., Bernier-Cardou, M., Isabel, N. et al. Effect of sugars, amino acids, and culture technique on maturation of somatic embryos of Pinus strobus on medium with two gellan gum concentrations. Plant Cell, Tissue and Organ Culture 62, 27–37 (2000). https://doi.org/10.1023/A:1006402215457

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