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Micropropagation of Pothomorphe umbellata via direct organogenesis from leaf explants

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Abstract

The establishment of a micropropagation protocol for Pothomorphe umbellata was carried out using leaf segments cultured on 1/4 strength Murashige and Skoog medium supplemented with 0.5 mg l-1 6-benzyladenine, 0.1 mg l-1 gibberelic acid added with 10 g l-1 sucrose. Rooting was achieved using MS medium devoid of growth regulators. An anatomical study confirmed shoot regeneration via direct organogenesis.

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Authors and Affiliations

  1. Departamento de Biotecnologia Vegetal -, UNAERP, 16096-380, Ribeirão Preto, SP, Brazil

    Ana Maria S. Pereira, Bianca W. Bertoni, Alba R. B. Araujo, Ana Helena Januário, Miriam V. Lourenço & Suzelei C. França

  2. Departamento de Botânica -, ESALQ/USP, Piracicaba, SP, Brazil

    Beatriz Appezzato-da-Glória

Authors
  1. Ana Maria S. Pereira
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  2. Bianca W. Bertoni
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  3. Beatriz Appezzato-da-Glória
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  4. Alba R. B. Araujo
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  5. Ana Helena Januário
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  6. Miriam V. Lourenço
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  7. Suzelei C. França
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Pereira, A.M.S., Bertoni, B.W., Appezzato-da-Glória, B. et al. Micropropagation of Pothomorphe umbellata via direct organogenesis from leaf explants. Plant Cell, Tissue and Organ Culture 60, 47–53 (2000). https://doi.org/10.1023/A:1006409807719

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  • DOI: https://doi.org/10.1023/A:1006409807719

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