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In vitro androgenesis of triticale in isolated microspore culture

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Abstract

Culture conditions for triticale (X Triticosecale Wittmack) androgenesis were studied using microspore culture. Sporophytic development of isolated triticale microspores in culture is described in five winter hexaploid triticale genotypes. Microspores were isolated using a microblendor, and embryogenesis was induced in modified 190-2 medium both in the presence and absence of growth regulators. The highest induction of microspore embryogenesis was obtained in a growth regulator-free medium. Adventitious embryogenesis was observed during in vitro development of triticale microspores. Albino and green plantlets were regenerated from embryo-like structures. More than 50% of regenerants were albino. In total, 126 green plantlets were produced, transplanted and established in soil. Cytological evidence revealed that 90% of the transplanted regenerants were haploid.

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References

  • Bajaj YPS (1990) In vitro production of haploids and their use in cell genetics and plant breeding. In: Bajaj YPS (ed.) Haploids in Crop Improvement I (pp 3–44). Biotechnology in Agriculture and Forestry. Springer-Verlag, Berlin

    Google Scholar 

  • Balatero CH, Darvey NL & Lucket DJ (1995) Genetic analysis of anther culture response in 6x triticale. Theor. Appl. Genet. 90: 279–284

    Article  Google Scholar 

  • Bernard S (1980) In vitro androgenesis in hexaploid triticale: determination of physical conditions increasing embryoid and green plant production. Z. Pflanzenzuchtg. 85: 308–321

    Google Scholar 

  • Borrino EM & Powell W (1988) Stomatalguard cell length as an indictor of ploidy in microspore-derived plants of barley. Genome 30: 158–160

    Article  Google Scholar 

  • Charmat G & Bernard S (1984) Diallel analysis of androgenetic plant production in hexaploid triticale (x Triticosecale, Wittmack). Theor. Appl. Genet. 69: 55–61

    Google Scholar 

  • De Buyser J, Hernry Y, Lonnet P, Hertzog R & Hespel A (1987) 'Florin': A doubled haploid wheat variety developed by the anther culture method. Plant Breeding 98: 53–56

    Article  Google Scholar 

  • Dunwell JM (1996) Microspore culture. In: Jain SM, Sopory SK & Veilleux RE (eds) In Vitro Haploid Production in Higher Plants, Vol 1 (pp. 205–216). Kluwer Academic Publishers, Dordrecht/Boston/London

    Google Scholar 

  • Fowler J & Cohen L (1990) Practical Statistics for Field Biology (pp. 1–227). Open Univ. Press, Milton Keynes, Philadelphia

    Google Scholar 

  • Gland-Zwerger A, Javornik B, Bohanec B & Kreft I (1994) Production of doubled haploid lines through anther culture in barley and triticale. In: Javornik B & Bohanec B (eds) Proceedings of the International Colloquium on Impact of Plant Biotechnology on Agriculture (pp. 9–14). Rogla, Slovenia, December 5–7

  • Harms CT & Potrikus I (1978) Fractionation of plant protoplast types iso-osmotic density gradient centrifugation. Theor. AppI. Genet. 53: 57–63

    Google Scholar 

  • Heberle-Bors E (1989) Isolated pollen culture in tabacco: plant reproductive development in a nutshell. Sex Plant Repro. 2: 1–10

    Google Scholar 

  • Hoekstra S, van Zijderveld MH, Louwerse JD, Heidekamp F & van der Mark F (1992) Anther and microspore culture of Hordeum vulgare L. cv. Igri. Plant Science 86: 89–96

    Article  CAS  Google Scholar 

  • Hu DF, Yuan ZD, Tang YL, Liu JP (1986) Jinghua No.1 a winter wheat variety derived from pollen sporophyte. Scientia Sinica B. XXIX: 733–745

    Google Scholar 

  • Immonen AST (1996) Triticale Breeding and Synthesis-Applications of Tissue Culture (pp 1–61). Acad. dissert. in plant breeding, Helsinki

  • Jähne A, Becker D, Brettschneider R & Lörz H (1994) Regeneration of transgenic, microspore-derived, fertile barley. Theor. Appl. Genet. 89: 525–533

    Article  Google Scholar 

  • Karsai I, Bedö Z & Hayes PM (1994) Effect of induction medium pH and maltose concentration on in vitro androgenesis of hexaploid winter triticale and wheat. Plant Cell Tiss. Org. Cult. 39: 49–53

    Article  CAS  Google Scholar 

  • Kiss A (1966) Neue Richtung in der Triticale-Zuchtung. Z. Pflanzenzuchtung 55: 309–329

    Google Scholar 

  • Keller J (1991) Influence of media conditioning on in vitro development in pollen suspensions of triticale. Biologisches Zentralblatt 110: 207–214

    Google Scholar 

  • Kiviharju E, Moisander S, Puolimatka M, Saastamoinen M, Hovinen S & Pehu E (1996) The effect of genotype on embryo production of oat (Avena sativa L.) anther culture. 2nd Int. Crop Sci. Congress, Nov. 17–24, New Delhi, India, Abs. Post. Sessions (p. 141)

  • Kush GS & Virmani SS (1996) Haploids in plant breeding. In: Mohan Jain S, Sopory SK & Veilleux RE (eds) In vitro production in higher plants, Vol 1 (pp. 11–34). Kluwer Academic Publishers, Dordrecht/Boston/ London

    Google Scholar 

  • Lelley T (1992) Triticale, still a promise? Plant Breeding 109: 1–17

    Article  Google Scholar 

  • Lelley T & Gimbel EM (1989) ‘Genome combining ability’ of wheat and rye in triticale. Plant Breeding 102: 273–280

    Google Scholar 

  • Lukjanjuk SF & Ignatova SA (1986) Triticale: production of haploid and homozygous plants. In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, Vol. 2 (pp. 530–543). Crops Springer, Berlin/Heidelberg/New York/Tokyo

    Google Scholar 

  • Martinez-Garcia C, Martin Sanchez JA & Sin Casas E (1992) Plant regeneration from anther culture in six hexaploid triticale varieties and their Fl hybrids (pp 189–190). Proc 18th Eucarpia Congress, July 6–11, Angers, France

  • Mordhorst AP & Lörz H (1993) Embryogenesis and development of isolated berley (Hordeum vulgare L.) microspores are influenced by the amount and composition of nitrogen sources in culture media. J. Plant Physiol. 14: 485–492

    Google Scholar 

  • Olsen FL (1991) Isolation and cultivation of embryogenic micropores from barley (Hordeum vulgare L.). Hereditas. 115: 255–266

    PubMed  CAS  Google Scholar 

  • Pauk J, Kertész Z, Beke B, Bóna L, Csösz M & Matuz J (1995) New winter wheat variety: ‘GK Délibáb’ developed via combining conventional breeding and in vitro androgenesis. Cer. Res. Comm. 23: 251–256

    Google Scholar 

  • Pauk J, Manninen O, Mattila I, Salo Y & Puli S (1991) Androgenesis in hexaploid spring wheat F2 population and their parents using a multiple-step regeneration system. Plant Breeding 107: 18–27

    Article  Google Scholar 

  • Puolimatka M, Laine S & Pauk J (1996) Effect of ovary cocultivation and culture medium on embryogenesis of directly isolated microspores of wheat. Cer. Res. Comm. 24: 393–400

    Google Scholar 

  • Rajaram S, Varughese G, Abdalla O, Pfeiffer WH & Ginkel MV (1993) Accomplishments and challenges in wheat and triticale breeding at CIMMYT. Plant Breeding Abstracts 63: 131–139

    Google Scholar 

  • Rimpau W (1891) Kreuzungsprodukte landwirtschaftlicher Kulturpflanzen II. Künstliche Kreuzungen von Weizen x Roggen. Landw. Jahrbuch. 20: 335–371

    Google Scholar 

  • Rines HW, Phillips RL & Somers D (1992) Application of tissue culture to oat improvement (pp 777–791). Oat Science and Techniology-Agronomy Monograph No.33, S. Segoe Rd., Madison

  • Schumann G & Hoffmann B (1989) Some pros and cons in using potato extract medium in anther culture of triticale and wheat. Archiv für Züchtungsforschung 19: 21–27

    Google Scholar 

  • Scott P & Lyne RL (1994) The effect of different carbohydrate sources upon the initiation of embryogenesis from barley microspores. Plant Cell Tiss. Org. Cult. 36: 129–133

    Article  CAS  Google Scholar 

  • Stolarz A & Lörz H (1986) Somatic embryogenesis in vitro manipulation and plant regeneration from immature embryos of hexaploid Triticale (X Triticosecale Wittmack). Z. Pflanzenzüchtung 96: 353–362

    Google Scholar 

  • Telmer CA, Newcomb W & Simmonds DH (1995) Cellular changes during heat shock induction and embryo development of cultured microspores of Brassica napus cv. Topas. Protoplasma 185: 106–112

    Article  Google Scholar 

  • Wang YB, Hu H & Snape JW (1996) The genetic and molecular characterization of pollen-derived plant lines from octoploid triticale x wheat hybrids. Theor. Appl. Genet. 92: 811–816

    Article  Google Scholar 

  • Wang YY, Sun CS, Wang CC & Chien WI (1973) The induction of the pollen plantlets of Triticale and Capsicum annum from anther culture. Scientia Sinica 16: 147–151

    Google Scholar 

  • Widholm JM (1972) The use of fluoresceine diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol. 47: 189–194

    PubMed  CAS  Google Scholar 

  • Zhuang JJ & Jia X (1983) Increasing differentiation frequencies in wheat pollen callus. In: Hu H & Vega MR (eds) Cell and Tissue Culture Techniques for Cereal Crop Improvement (p 431). Science Press, Beijing

    Google Scholar 

  • Ziauddin A, Marsolais A, Simion E & Kasha KJ (1992) Improved plant regeneration from wheat anther and barley microspore culture using phenylacetic acid (PAA). Plant Cell Rep. 11: 489–498

    Article  CAS  Google Scholar 

  • Zimny J, Becker D, Brettschneider R & Lörz H (1995) Fertile transgenic Triticale (X Triticosecale Wittmack). Molecular Breeding 1: 155–164

    Article  Google Scholar 

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Correspondence to János Pauk.

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Pauk, J., Puolimatka, M., Lökös Tóth, K. et al. In vitro androgenesis of triticale in isolated microspore culture. Plant Cell, Tissue and Organ Culture 61, 221–229 (2000). https://doi.org/10.1023/A:1006416116366

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