Abstract
Totipotent calli of a Paphiopedilum hybrid (Paphiopedilum callosum ‘Oakhi’ × Paph. lawrenceanum ‘Tradition’) were induced from seed-derived protocorms on a 1/2 strength Murashige–Skoog medium plus 1–10 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1–1 mg l−1 1-phenyl-3-(1.2.3-thiadiazol-5-yl)urea (TDZ). These calli grew well when subcultured on the same medium, but proliferated more on 1/2 MS medium plus 5 mg l−1 2,4-D and 1 mg l−1 TDZ. Calli developed further along a route of production of protocorm-like bodies and eventually formed plantlets that could be transplanted to pots and grew well.
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Lin, YH., Chang, C. & Chang, WC. Plant regeneration from callus culture of a Paphiopedilum hybrid. Plant Cell, Tissue and Organ Culture 62, 21–25 (2000). https://doi.org/10.1023/A:1006425214471
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DOI: https://doi.org/10.1023/A:1006425214471