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In vitro organogenesis and plant formation in Acacia sinuata

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Abstract

In vitro morphogenesis via organogenesis was achieved from callus cultures derived from hypocotyl explants of Acacia sinuata on MS (Murashige and Skoog, 1962) medium. Calli were induced from hypocotyl explants excised from 7-day-old seedlings on MS medium containing 3% sucrose, 0.8% agar, 6.78 μM 2,4-dichlorophenoxyacetic acid and 2.22 μM 6-benzylaminopurine. Regeneration of adventitious buds from callus was achieved when they were cultured on MS medium supplemented with 10% coconut water, 13.2 μM 6-benzylaminopurine and 3.42 μM indoleacetic acid. Addition of gibberellic acid (1.73 μM) favored shoot elongation. Regenerated shoots produced prominent roots when transferred to half strength MS medium supplemented with 7.36 μM indolebutyric acid. Rooted plantlets, thus developed were hardened and successfully established in the soil. This protocol yielded an average of 20 plants per hypocotyl explant over a period of 4 months.

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Authors and Affiliations

  1. Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirapalli, 620 024, Tamilnadu, India

    G. Vengadesan, A. Ganapathi, R. Prem Anand & V. Ramesh Anbazhagan

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  1. G. Vengadesan
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  2. A. Ganapathi
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  3. R. Prem Anand
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  4. V. Ramesh Anbazhagan
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Correspondence to A. Ganapathi.

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Vengadesan, G., Ganapathi, A., Prem Anand, R. et al. In vitro organogenesis and plant formation in Acacia sinuata. Plant Cell, Tissue and Organ Culture 61, 23–28 (2000). https://doi.org/10.1023/A:1006442818277

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  • DOI: https://doi.org/10.1023/A:1006442818277

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