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Micropropagation of Indian wild strawberry

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Abstract

An efficient method of micropropagation based on an increased percentage survival of explants and reduced phenol-induced browning in wild strawberry has been developed. Serial transfer of nodal explants was carried out at 24-, 48- and 96-h intervals. Nodal segments cultured on Murashige and Skoog medium supplemented with 6-benzyladenine (4.0 μM) and α-naphthalene acetic acid (0.1 μM) gave the best (94.4%) explant establishment and shoot number (22.3) per explant. Of the cytokinins tested, 6-benzyladenine was found more effective than kinetin and N6-(γ,γ dimethylallyamino) purine. Excised shoots rooted on half-strength agar-gelled medium with 1.0 μM α-naphthalene acetic acid. Rooted shoots with fully expanded leaves acclimatized successfully and about 70% of plantlets survived ex vitro.

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Authors and Affiliations

  1. G.B. Pant Institute of Himalayan Environment & Development, Kosi-Katarmal, Almora, 263643 (U.P.), India

    Indra D. Bhatt, Uppeandra Dhar & Uppeandra Dhar

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  1. Indra D. Bhatt
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  2. Uppeandra Dhar
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Correspondence to Uppeandra Dhar.

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Bhatt, I.D., Dhar, U. Micropropagation of Indian wild strawberry. Plant Cell, Tissue and Organ Culture 60, 83–88 (2000). https://doi.org/10.1023/A:1006471815566

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  • DOI: https://doi.org/10.1023/A:1006471815566

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