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The effects of silver nitrate and different carbohydrate sources on somatic embryogenesis in Coffea canephora

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Abstract

The response of five Coffea canephora Pierre genotypes with regard to somatic embryogenesis was tested on media containing silver nitrate (AgNO3) and different carbohydrates (sucrose, fructose, maltose and glucose). The presence of AgNO3 caused only small modifications to the ionic equilibrium of the media. At concentrations between 30–60 μM, AgNO3 improved embryo yield for the genotypes evaluated, while higher doses negatively affected the regenerative capacity. The substitution of maltose, glucose or fructose for sucrose produced different responses depending on the genotype. Fructose significantly increased somatic embryo production in genotypes N91 and N128, while maltose was highly effective for N75. In addition, more synchronous embryo development was observed in genotype N91 when glucose was used instead of sucrose.

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Authors and Affiliations

  1. Instituto Agronômico do Paraná, Área de Ecofisiologia, Caixa Postal 481, 86001-970, Londrina, PR, Brazil

    Sandra R.L. Fuentes, Maria B.P. Calheiros, João Manetti-Filho & Luiz G.E. Vieira

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  1. Sandra R.L. Fuentes
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  2. Maria B.P. Calheiros
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  3. João Manetti-Filho
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  4. Luiz G.E. Vieira
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Correspondence to Luiz G.E. Vieira.

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Fuentes, S.R., Calheiros, M.B., Manetti-Filho, J. et al. The effects of silver nitrate and different carbohydrate sources on somatic embryogenesis in Coffea canephora. Plant Cell, Tissue and Organ Culture 60, 5–13 (2000). https://doi.org/10.1023/A:1006474324652

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