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AFLP Fingerprinting for Assessing Intraspecific Variation and Genome Mapping in Mites

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Abstract

Molecular genetic techniques have come a long way in the last decade. With the advent of PCR, genetic markers are now accessible for all organisms, including mites. However, there is usually a trade-off between the accuracy of the molecular technique or genetic marker and expediency. In mites, many molecular techniques are not applicable due to their small size. Here we describe a relatively new molecular fingerprinting technique, amplified fragment length polymorphism (AFLP), which is currently used widely in plant genomic research. We outline the AFLP procedure adapted for mites, show results using this technique from our own research and discuss the benefits and limitations of AFLPs for assessing genetic variation and for genome mapping. It is our intention to highlight the possible use of AFLPs as genetic markers with a broad application in acarological research.

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Authors and Affiliations

  1. Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Kruislaan 320, 1098 SM, Amsterdam, the Netherlands

    A.R. Weeks

  2. Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Kruislaan 320, 1098 SM, Amsterdam, the Netherlands

    T. van Opijnen & J.A.J. Breeuwer

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  1. A.R. Weeks
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  2. T. van Opijnen
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  3. J.A.J. Breeuwer
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Weeks, A., van Opijnen, T. & Breeuwer, J. AFLP Fingerprinting for Assessing Intraspecific Variation and Genome Mapping in Mites. Exp Appl Acarol 24, 775–793 (2000). https://doi.org/10.1023/A:1006486400800

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